Direct conversion of neurons to fibroblastssyed shafiqThe document summarizes a research article that was published in Nature in 2010. The study found that fibroblasts could be directly converted into functional neurons by infecting the cells with viruses containing five transcription factor genes (Ascl1, Brn2, Myt1l, Zic1, and Olig2). The resulting induced neuronal (iN) cells displayed neuronal morphology, membrane properties, and ability to form functional synapses similar to primary neurons. Further experiments showed that Ascl1 alone or Ascl1 combined with Brn2 and Myt1l were able to generate iN cells, demonstrating the key factors required for direct neuronal conversion.
Neuronal stem cellfiDUVASUThis document provides an overview of neural stem cells (NSCs). It discusses the location and development of NSCs in the central nervous system. Key cell signaling pathways that regulate NSCs, such as Notch and WNT, are described. Common markers used to identify NSCs are discussed, including Nestin, Sox2, Musashi-1, Pax6, and CD133. Factors that affect the growth and multiplication of NSCs, such as growth factors EGF and FGF, are outlined. Methods for isolating and culturing NSCs are presented. Finally, potential therapeutic applications of NSCs are reviewed, along with some current clinical trials utilizing NSCs.
Single cell RNA sequencing; Methods and applicationsfaraharoojݺߣs explains all methods of single cell RNA sequencing and their applications in health and research.
