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- 2. Folyad辿kkromatogr叩fia - t旦megspektrometria (LC-MS) analitikai k辿miai technika, amely a folyad辿kkromatogr叩fi叩s (LC) fizikai elv叩laszt叩si k辿pess辿geit 辿s a t旦megspektrometria (MS) t旦megelemz辿si k辿pess辿geit 旦tv旦zi az 1980-as 辿vekben indult sok vegy端let m辿rhet egyetlen analitikai futtat叩ssal nagyon 辿rz辿keny 辿s szelekt鱈v hasznos sz叩mos alkalmaz叩sban
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Editor's Notes
- #9: 油油ESI operates according to the following scheme: 1) the column effluent from the LC containing analytes is pumped through a capillary which is held at high potential (2-6 kV) and nebulized. The applied voltage can be either positive or negative, depending on the analytes; nebulization is usually assisted pneumatically (except in nanospray); 2)油the droplets that detach from a tip of the capillary contain an excess of positive or negative charge as a result of the applied high voltage; 3) electrical field gradient attracts charged droplets towards the entrance of the mass spectrometer; 4) charged analyte molecules are generated from the small charged droplets either by the charged residue model or by the ion evaporation model. Ion formation from the droplets is promoted by a flow of油drying gas (usually heated nitrogen); 5) the ions, solvent vapor and drying gas molecules are sampled through a capillary into a first pumping stage (0.08-0.75 Torr) where they are supersonically expanded; 6)油the ions and some other neutral molecules are sampled via a skimmer into the second pumping stage (0.001-0.01 Torr) containing an ion focusing and transfer device (usually RF hexapole or octapole and a set of lenses); 7)油ions enter mass analyzer region (< 10-5 Torr).
- #10: 油油ESI operates according to the following scheme: 1) the column effluent from the LC containing analytes is pumped through a capillary which is held at high potential (2-6 kV) and nebulized. The applied voltage can be either positive or negative, depending on the analytes; nebulization is usually assisted pneumatically (except in nanospray); 2)油the droplets that detach from a tip of the capillary contain an excess of positive or negative charge as a result of the applied high voltage; 3) electrical field gradient attracts charged droplets towards the entrance of the mass spectrometer; 4) charged analyte molecules are generated from the small charged droplets either by the charged residue model or by the ion evaporation model. Ion formation from the droplets is promoted by a flow of油drying gas (usually heated nitrogen); 5) the ions, solvent vapor and drying gas molecules are sampled through a capillary into a first pumping stage (0.08-0.75 Torr) where they are supersonically expanded; 6)油the ions and some other neutral molecules are sampled via a skimmer into the second pumping stage (0.001-0.01 Torr) containing an ion focusing and transfer device (usually RF hexapole or octapole and a set of lenses); 7)油ions enter mass analyzer region (< 10-5 Torr).