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This document discusses the development of e-beam lithography gold nanoparticle arrays for biosensor applications. It begins by explaining why biosensors are important for healthcare and environmental safety due to their potential for high performance, selectivity, sensitivity, low cost and fast detection. It then provides details on the fabrication process for the gold nanoparticle arrays using e-beam lithography on glass substrates. This includes steps like PMMA coating, e-beam exposure, development and gold deposition. Finally, it discusses future applications like developing biosensors for disease detection and cooperating with biochemistry groups to integrate recognition elements.
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This document discusses biosensors, including their definition, components, basic characteristics, advantages, sensing techniques, types, and applications. A biosensor is defined as a self-contained integrated device that uses a biological recognition element in direct contact with a transduction element to provide analytical information. Professor Leland C. Clark Jr. is considered the father of biosensors. Biosensors have various advantages such as high specificity, independence from factors like pH, linear response, small size, durability, and rapid results. Common sensing techniques include fluorescence, DNA microarrays, SPR, impedance spectroscopy, and electrochemical methods. Major types include optical, resonant, piezoelectric, ion-sensitive, and electrochemical biosensors. Biosensors亳仂仗仍舒亰仄仂仆亳从舒 - 弍亳仂亳仗 亟仍 从仗亠-亟亳舒亞仆仂亳从亳 仗舒仂仍仂亞亳亶 仂亞舒仆亳亰仄舒 亳 亰舒弍仂仍亠...
亳仂仗仍舒亰仄仂仆亳从舒 - 弍亳仂亳仗 亟仍 从仗亠-亟亳舒亞仆仂亳从亳 仗舒仂仍仂亞亳亶 仂亞舒仆亳亰仄舒 亳 亰舒弍仂仍亠...Dmitriy Filippov
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This document discusses the development of biochips for analyzing metabolic imbalances in humans. It notes that 350 million people have diabetes worldwide and there is an urgent need for reliable disease prediction and risk assessment. The proposed solution is biochips that can quantitatively measure glycated hemoglobin and albumin levels using surface-enhanced Raman scattering (SERS) for highly sensitive detection. The biochips would be inexpensive, noninvasive, and easy to use. A team is presented that would develop and test the biochips over a 6 year period, with the goal of licensing the technology to diagnostic companies in years 3-6.Current and future techniques for cancer diagnosis
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This document discusses various nanotechnology approaches for cancer diagnosis, including the use of gold nanoparticles, quantum dots, carbon nanotubes, and nanoflares. Gold nanoparticles can be used for detection through techniques like dynamic light scattering and surface plasmon coupling. Quantum dots and carbon nanotubes can also be functionalized for ultrasensitive detection of cancer biomarkers. Emerging tools like nanoflares allow for detection of genetic targets associated with cancer within living cells. Overall, nanotechnology enables low detection limits and early cancer diagnosis.Nano medicine and drug delivery in biomedical imaging
Nano medicine and drug delivery in biomedical imagingrobitaco
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Nanomedicine offers advantages for cancer treatment through nanoscale devices that can enter cells easily and transport drugs. Quantum dots and gold nanoshells show promise as contrast agents for imaging. Nanoparticles, nanowires, and nanocantilevers may enable early cancer detection. Future applications could include artificial red blood cells that transport more oxygen, and nanobots to repair damaged cells. Overall, nanotechnology provides methods for prevention, detection, imaging, and targeted treatment of cancer at the molecular level.Biosensors
BiosensorsRujamKennady
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This document discusses biosensors, including their definition, components, working principles, characteristics, types, advantages, and applications. A biosensor consists of a bioreceptor and transducer, where the bioreceptor undergoes a biological reaction in response to an analyte and the transducer converts this reaction into a measurable electrical signal. The document outlines the key components of biosensors and how they function, describing various types including electrochemical, optical, and ion-sensitive biosensors. It notes biosensors offer advantages like high sensitivity and selectivity. Finally, the document lists applications of biosensors in fields like healthcare, environmental monitoring, food analysis, and more.23MBT10068nanotechnology and practical.pptx
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Biosensors combine biological components with physicochemical detectors to detect and quantify analytes. They utilize biological elements like enzymes, antibodies, or nucleic acids for specific detection of targets. The biological response is converted to a measurable signal via a transducer and processed for output. Biosensors are used in healthcare for tests like blood glucose monitoring, in environmental monitoring for pollutants, and in food safety for pathogens. Advancements in nanomaterials and integrated systems promise more sensitive, selective, and portable biosensors for point-of-care applications and personalized medicine in the future.Biosensors & its applications
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This document summarizes biosensors and their applications. It defines a biosensor as a device that integrates a biological recognition element with a transducer to provide analytical information. Professor Leland C. Clark Jr. is considered the father of biosensors for inventing the Clark electrode to measure oxygen in blood and liquids. Biosensors are used in medicine, environmental monitoring and industry to detect and quantify materials. Examples discussed include glucose monitoring devices, pregnancy tests, and sensors for tuberculosis, toxicants, and mercury. The document also outlines the basic components and working principles of biosensors.Open Frame Sequencing 
Open Frame Sequencing INVICTA GENETICS
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Open Frame Sequencing is a universal tool that allows planning comprehensive genetic diagnostics personalized for each Patient. This solution is dedicated to specialists who expect flexible approach, efficient cooperation and tailor made solutions in their daily work. Biosensor .pptx
Biosensor .pptxCHIRANTANMONDAL2
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A biosensor is a device that uses biological components like enzymes, antibodies or nucleic acids to detect analytes. It has a biological recognition element connected to a transducer that converts the biological response into an electrical signal. There are various types of biosensors based on the recognition element like enzymes, microbes, antibodies etc. and the transducer used like potentiometric, amperometric, optical etc. Biosensors have applications in medical diagnostics, food analysis, environmental monitoring and various other fields due to their specificity, sensitivity and portability.Nanotechnology in microbiology
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The document discusses various applications of nanotechnology in microbiology. It begins by defining nanotechnology as the manipulation of matter at the nanoscale of 1 to 100 nm. Some key applications discussed include using quantum dots for pathogen detection through fluorescence, using gold and silver nanoparticles in assays like sol particle immunoassays, and using magnetic nanoparticles in detection methods like magnetic relaxation switches that can detect as few as 5 viral particles. The document also discusses nanoparticle-based methods that enable faster, more sensitive detection of pathogens without sample preparation.role of nanotechonolgy in diagnostic pathology.pptx
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Nanotechnology involves engineering materials and devices on the nanoscale (1-100 nm) and can be applied to diagnostic pathology. It allows for early disease detection by taking advantage of nanoparticles' high surface area and ability to interact with biomolecules. Common nanodiagnostic techniques include magnetic nanoparticles for MRI contrast, nanochips for DNA analysis, microfluidics to automate biochemical assays, and nanoarrays for high-throughput screening of proteins and nucleic acids. Future applications may integrate multiple functions like imaging, detection, targeted drug delivery, and treatment monitoring into single nanodevices.China Medical University Student ePaper2
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Microarray and bio-chips provide a new technology for analyzing samples in an instant, automatic, and high-efficiency way. Microarray biochips can be divided into DNA chips and protein chips. DNA chips use nucleic acids as probes to examine thousands of genes simultaneously, while protein chips use proteins, antibodies, or microorganisms as probes to detect factors like hormones. Microarray biochips allow many samples, reagents, and biological materials to react on a small, miniaturized device, generating data immediately after quantitative analysis. This technology is being developed for uses in medical diagnostics and drug development.Rajul use of biosensors in agriculure
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This document discusses biosensors and their applications in agriculture. It begins with defining biosensors as integrated devices that use a biological recognition element in direct contact with a transducer to provide analytical information. It then describes the basic principles and components of biosensors, including immobilization of biological material, interaction with analytes, and signal conversion by transducers. The document outlines characteristics like linearity, sensitivity and selectivity. It discusses various types of biosensors and their advantages. Finally, it provides examples of biosensor applications for detecting pesticides, herbicides and other agricultural pollutants, as well as their use in environmental monitoring and food analysis.mHealth Israel_Ryo Kosaka_AIST_National Institute of Advanced Industrial Scie...
mHealth Israel_Ryo Kosaka_AIST_National Institute of Advanced Industrial Scie...Levi Shapiro
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Presentation by Ryo Kosaka, Senior Research Scientist, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST). Includes an overview of priority strategies in Life Sciences and Biotech and description of the organization of the Life Sciences and Biotech department. Recent projects include a Portable System for High-Speed DNA Quantification, Application of a cell microarray chip for clinical diagnosis and single cell analysis, Safe and Secure Artificial Heart, New diagnosis for liver fibrosis utilizing glycans, AIST ventures from the department of Life Science & Biotech as well as International cooperation. Breathsafe- a biosensor designed for detecting H1N1
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The document describes a biosensor called BREATHSAFE速 for detecting H1N1 infections. It uses anti-hemagglutinin and anti-neuraminidase protein fragments immobilized on a glass slide as bioreceptors to detect the H1N1 viral proteins hemagglutinin and neuraminidase in respiratory secretions. When the viral proteins bind to the bioreceptors, secondary antibodies tagged with fluorophores bind, and fluorescence is excited and detected with a CCD camera, allowing for diagnosis. The sensor hardware will be inexpensive to manufacture and the disposable membranes will generate continuous revenue.Plos
PlosCarolyn Salvi
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This research article describes a novel method using high-density peptide microarrays and computational analysis to identify B-cell epitopes in patients with celiac disease. Overlapping peptide sequences from native and deamidated gliadin proteins were synthesized onto silicon wafers. Serum samples from celiac patients and controls were tested on the microarrays. Computational analysis identified distinct epitope sets that differentiated celiac patients from controls with high accuracy. The identified epitopes have potential for developing improved diagnostic tests for celiac disease.BIOSENSORS AND NANOTECH OVERVIEW for the university level
BIOSENSORS AND NANOTECH OVERVIEW for the university levelSandipPradhan23
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nothing to as usual as the ppt readingMore from Maher Selim (8)
Maher_Selim_Resume
Maher_Selim_ResumeMaher Selim
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Maher Selim is a research scientist with a PhD in Physics from Western University and experience conducting experimental, theoretical, and computational research to develop standards and measurement techniques for nanotechnology as a researcher at the National Institute of Standards in Egypt. He has skills in teaching, problem solving, research, analytics, and team leadership and has published research papers on topics like graphene and magnetic nano-ribbons.Maher_Selim_Researcher_CV
Maher_Selim_Researcher_CVMaher Selim
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Maher Selim is a research scientist living in Peterborough, Ontario, Canada. He received his PhD in Physics from Western University in 2012 and has worked as a researcher at the National Institute of Standards in Egypt. He has skills in teaching, research, problem solving, team leadership, and staff management. His research focuses on nanotechnology, including band gap engineering of graphene and magnetic nano-ribbons. He is fluent in English and Arabic and has published several journal articles and conference papers.
Teaching Dossier
Teaching DossierMaher Selim
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This document outlines Maher Selim's teaching experience and philosophy. It details the courses he has taught, including physics courses at various universities from 2001-2009. It describes his role as a teaching assistant and demonstrator. His teaching philosophy focuses on engaging students through active and exploratory learning methods that relate physics concepts to real-life examples and problems. He aims to develop students' practical and creative thinking skills.
msc3.PPT
msc3.PPTMaher Selim
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This document evaluates different methods for determining the moisture content in wood and recommends the best method. It summarizes the process undertaken to establish a calibration system for moisture content meters at the National Institute for Standards in Egypt. Oven-drying wood samples was used as the standard method to calibrate two types of electric moisture meters: conductance and capacitance meters. Samples of various wood types were conditioned at different relative humidities and their moisture contents were measured using the meters and oven-drying for comparison. The calibration system aims to reduce errors and uncertainties in moisture content measurements.photonic course2
photonic course2Maher Selim
油
The document discusses the empirical design of gold nanoparticle arrays for biosensors. It examines different factors that affect the localized surface plasmon resonance (LSPR) peak wavelength of gold nanoparticles, including substrate material, nanoparticle size, shape, interparticle distance, and aggregation. Glass is identified as the most suitable substrate due to its absorption peak. Simulations using Mie theory and the discrete dipole approximation predict that increasing nanoparticle size, changing shape from spherical to cylindrical, decreasing interparticle distance, and particle aggregation will result in a red shift of the LSPR peak wavelength.
advisory committee
- 1. E-beam lithography gold nanoparticle arrays for nano-biosensor development Maher Z. Ahmed PhD research project
- 2. Health care Environment safety Why biosensor? High performance bio and chemical sensor Selective Sensitive Easy to use Cheap Fast
- 3. What is a biosensor?
- 4. Enhance selectivity very high selective chemistry. DNA strains or specialist enzyme The advanced new sensors Researches Enhance sensitivity improving coupling between the chemical detection and transducer improving transducer Physics of nanoparticles offers a promising future in that field. Top-Down approach Bottom-up approach
- 5. Enhance sensitivity Signal Acquisition The advanced new sensors Researches advanced technology in optical spectroscopy instrumentations
- 6. Au catcher or probe oligonucleotides biotin-coupling group S Streptavidin, biotin biotin-terminated thiol biotin Glass substrate complementary oligonucleotide target Light My Target biosensor?
- 7. Healthy Patient Haes AJ, Chang L, Klein WL, Van Duyne RP. 2005. Detection of a biomarker for Alzheimers disease from synthetic and clinical samples using a nanoscale optical biosensor. J. Am. Chem. Soc. 127:226471
- 8. Songbo Xu PhD. thesis Surface Plasmon Spectroscopy (SPS) to monitor the bio chemistry reaction
- 9. Songbo Xu PhD. thesis W. Knoll et al. : Colloids and Surfaces A: Physicochem. Eng. Aspects 161 (2000)
- 10. 1- e-beam lithography system is a LEO 1530 field emission scanning electron microscope (FE-SEM) 2- Solitec 5110 Spinner/Developer 3-Custom e-beam Deposition (Hoser) 4- Zeiss Axioskop2 Mat Microscope E-beam lithography Nano gold particle arrays Fabrication and characterization (NanoFab)
- 11. E-beam lithography Nano gold particle arrays e _ Glass substrata PMMA E-beam lithography Developing Chromium Chromium Gold Lift Off
- 12. Signal Acquisition Old sample cuvette Needs for New design Minimum Volume target
- 13. Who Wants to Be a Millionaire? Future Applications for our optimum system
- 14. Cooperation with Dr. Bulent Mutus, Biochemistry Research Group, U. Windsor Au AU type 2 diabetes indications enzyme Cutting the double S-S bond Type 2, where the body makes insulin but cannot use it properly Absorbance vs Wavelength in 100 亮M GSH 0 0.05 0.1 0.15 0.2 0.25 0.3 400 450 500 550 600 650 700 750 800 Wavelength (nm) Absorbance(a.u.)