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Charles Proposal Presentation escher.pptx
- 1. Title: THE ANTIMICROBIAL-RESISTANT STRAINS OF PATHOGENIC ESCHERICHIA COLI ISOLATED FROM NAKURU COUNTY ABATTOIR WASTEWATERS Presented By: Charles Ndungu Kibuthu
- 2. Introduction 2 Global Meat Consumption Increase: 40% rise leading to 2021 Impact on Waste Production: Increase in meat-processing waste Public Health Concern: Pathogenic waste poses risks to humans, animals, and the environment
- 3. Research Problem Abattoir waste harbors enteric pathogens Enteric infections from abattoir wastewater Antibiotic-resistant E. coli pathotypes A point source of antimicrobial-resistant pathogens 3
- 4. Research Problem Abattoir Waste Composition: Includes wastewater, waste meat, fecal material Public Health Burden: Harbors pathogenic enteric microbes, risk of antibiotic resistance Need for Sustainable Management: Effective waste treatment is crucial 4
- 5. Questions 1.What is the occurrence of E. coli in Nakuru county abattoir wastewaters? 2.What is the antimicrobial sensitivity profile of these E. coli isolates? 3.What are the prevalent pathotypes of antimicrobial-resistant E. coli in these wastewaters? 6
- 6. Objectives General Objective Determine the antibiotic-resistant profiles of pathogenic E. coli from abattoir wastewaters. Specific Objectives Isolate and identify E. coli from wastewater samples. Characterize antibiotic resistance profiles. Classify antimicrobial-resistant E. coli based on virulence genes. 7
- 7. Justification of the Study Urgent Need: Rising demand for meat leads to more contaminated waste Public Health Risk: Potential outbreaks of antimicrobial-resistant infections Study Aim: Investigate pathogenic E. coli presence and antibiotic sensitivity profiles 8
- 8. Methodology Study Site: Nakuru County, Kenya Sample Collection: sample 67 abattoirs Isolation and Identification: Cultural methods and PCR-based molecular assays Antibiotic Sensitivity Testing: Kirby-Bauer disk diffusion method 10
- 9. Expected Results - Isolation of E. coli: From abattoir wastewater samples - Antibiotic Resistance Profiles: Detailed sensitivity profiles of isolated strains - Pathogenic Strains: Identification of antimicrobial- resistant E. coli strains 11
- 10. Significance of the Study - Understanding Resistance Dynamics: Insight into antibiotic resistance in abattoir wastewater - Public Health Recommendations: Inform strategies to prevent antimicrobial-resistant infections - Further Research: Basis for national prevalence studies 12
- 11. Conclusion Summary: Rising meat consumption increases waste and public health risks Importance of Study: Critical to address antibiotic resistance in E. coli from abattoir wastewaters Future Steps: Develop and implement effective waste management and decontamination strategies 13
- 12. THANK YOU Presented By : Charles Ndungu Kibuthu
- 13. References Yamane, Y. (1967). Mathematical formulae for sample size determination. J. Mathemetics, 1, 1-29.
Editor's Notes
- #3: The study: Isolates E. Coli in Nakuru County wastewater Tests the isolates response to antimicrobial agents Isolates antimicrobial resistance profiles Classifies the pathotypes of resistant isolates
- #5: Antimicrobial resistance burden undefined Waste decontamination and containment - not effective The need to develop better waste management techniques To prevent outbreaks of antimicrobial-resistant E. coli To understand the role of abattoirs in AMR
- #8: The presence of antimicrobial-resistant E. coli pathotypes Biological wastes in Nakuru County abattoirs Link to enteric infection cases Abattoir wastewater can harbor pathogenic E. Coli Presence of antimicrobial resistance -油 significance Impact on the油 health system Impact on literature Impact on waste management practices
- #9: Study site Nakuru county Study Approach and Design Qualitative - case study Cross-sectional data collection - laboratory data Convenient selection of sample collection sites Sample collection: Sample collected from slaughterhouses Convenient selection of slaughterhouses Specimen collected aseptically by grab method During peak work hours Transported/stored for less than 8 hours before processing Convenient selection of collection sites Aseptic techniques for specimen collection Grab method during peak hours - weekdays Sample size - Yamanes formula - Yamane (1967): Inclusion Samples collected in outfall locations in the abattoir drainage system Samples held at less than 10属C for at most 8 hours Sample collected through aseptic technique Samples collected before wastewater decontamination treatments Exclusion Samples not collected from outfall locations Samples not collected aseptically Samples held for longer than 8 hours at temperatures exceeding 10属C . Samples from recently treated wastewater Isolation and Identification Sample filtration via 0.45 micron membrane filters Plating in EMB agar for 24 hours Colony transfer to nutrient agar Biochemical characterization AST Kirby-Bauer disk diffusion method On Mueller-Hinton agar For common antibiotics PCR PCR-based molecular characterization - 16S rRNA gene marker Target pathotypes EHEC/STEC, EPEC, EIEC, and ETEC Specific primers will be used
- #10: Isolation of E. coli from abattoir wastewater Antimicrobial sensitivity profile of isolated E. coli Determination of the presence of antimicrobial-resistant pathotypes
- #11: Significance This is an opportunity to determine the presence of antimicrobial resistance Learn whether pathotypes exhibit antimicrobial resistance