Ecologisch ontwerpen voor onze toekomstakkernaarbos
Ìý
This document discusses sustainable agricultural production and agroecology. It begins by outlining the intensification discourse around needing to double food production by 2050 to feed a growing population. However, it argues this narrative is false and advocates for local solutions to the global food security problem. The document then discusses various agroecological practices and systems that can support sustainable intensification, including crop-livestock integration, agroforestry, and landscape-level approaches to biodiversity conservation and ecosystem service provision. It emphasizes co-creating knowledge with farmers and a transition towards more sustainable food systems.
20th. Single Molecule Workshop Picoquant 2014Dirk Hähnel
Ìý
SIMA is a novel software tool that enables automated single-molecule spectroscopy experiments on surface-immobilized molecules. It locates target molecules, plans an optimal measurement procedure by configuring detector exposure, excitation events, and position control. SIMA then executes the measurements by navigating through the sample space and controlling the positioning system in real-time for each molecule position. This allows complex measurements to be conducted with ease while saving time and minimizing photobleaching compared to manual experiments. As a demonstration, SIMA was used to simultaneously measure the three-dimensional orientation of the absorption and emission dipoles of single fluorescing molecules by combining point spectroscopy with localized wide-field imaging.
1. Image scanning microscopy (ISM) is a fluorescence microscopy technique that enhances spatial resolution approximately twofold compared to confocal laser scanning microscopy (CLSM).
2. ISM works by combining focused laser excitation with wide-field detection using an EMCCD camera. Images are taken at various scan positions of the excitation focus and combined to form a higher resolution composite image, similar to structured illumination microscopy (SIM).
3. ISM has advantages over SIM in that it is more robust against optical aberrations and imperfections since it does not require patterned illumination. The ISM setup can acquire super-resolution 3D multi-color images and optionally modulate excitation intensity for further resolution gains from non-linear effects
21st International Workshop on“Single Molecule Spectroscopy and Super-resolu...Dirk Hähnel
Ìý
Filling the usability gap: Bioinformatics solutions for Image-Scanning Microscopy, Stochastic Optical Fluctuation Imaging, and Surface Single Molecule Experiments
Keynote speech by Mark Shepard on november 24, 2015 in Wageningen University, The Netherlands and on the Van Akker naar Bos (from Field to Forest) conference on november 28 2015
The speaker expresses their deep love and gratitude for Beckie. Over the past four years, the speaker thought often of Beckie and prayed for her during difficult times. The speaker was reminded of their own past challenges when seeing Beckie's heart get crushed. They asked God to help free Beckie from chaos, and although there are not enough words, the speaker loves Beckie more with each day and breath.
20th. Single Molecule Workshop Picoquant 2014Dirk Hähnel
Ìý
SIMA is a novel software tool that enables automated single-molecule spectroscopy experiments on surface-immobilized molecules. It locates target molecules, plans an optimal measurement procedure by configuring detector exposure, excitation events, and position control. SIMA then executes the measurements by navigating through the sample space and controlling the positioning system in real-time for each molecule position. This allows complex measurements to be conducted with ease while saving time and minimizing photobleaching compared to manual experiments. As a demonstration, SIMA was used to simultaneously measure the three-dimensional orientation of the absorption and emission dipoles of single fluorescing molecules by combining point spectroscopy with localized wide-field imaging.
1. Image scanning microscopy (ISM) is a fluorescence microscopy technique that enhances spatial resolution approximately twofold compared to confocal laser scanning microscopy (CLSM).
2. ISM works by combining focused laser excitation with wide-field detection using an EMCCD camera. Images are taken at various scan positions of the excitation focus and combined to form a higher resolution composite image, similar to structured illumination microscopy (SIM).
3. ISM has advantages over SIM in that it is more robust against optical aberrations and imperfections since it does not require patterned illumination. The ISM setup can acquire super-resolution 3D multi-color images and optionally modulate excitation intensity for further resolution gains from non-linear effects
21st International Workshop on“Single Molecule Spectroscopy and Super-resolu...Dirk Hähnel
Ìý
Filling the usability gap: Bioinformatics solutions for Image-Scanning Microscopy, Stochastic Optical Fluctuation Imaging, and Surface Single Molecule Experiments
Keynote speech by Mark Shepard on november 24, 2015 in Wageningen University, The Netherlands and on the Van Akker naar Bos (from Field to Forest) conference on november 28 2015
The speaker expresses their deep love and gratitude for Beckie. Over the past four years, the speaker thought often of Beckie and prayed for her during difficult times. The speaker was reminded of their own past challenges when seeing Beckie's heart get crushed. They asked God to help free Beckie from chaos, and although there are not enough words, the speaker loves Beckie more with each day and breath.
Revenue and financing models for ecological farmersakkernaarbos
Ìý
This document discusses various revenue and financing models for ecological farms. It begins by introducing the speaker and stating that the afternoon will focus on inspirational models for revenue and financing. It then contrasts traditional annual crop farming with ecological polyculture farming, noting that the latter has smaller diverse harvests that are more difficult to sell and calculate income from. The rest of the document proposes several ideas for marketing ecological farm produce, including selling to local shops and farms, providing care services using the health benefits of nature, partnering with nature organizations, offering workspaces, creating experiences for customers, selling online, forming collectives, using local currencies, and cooperating and crowdfunding. It concludes by having participants brainstorm a plan to increase revenue