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Bioconversion of      Lignocellulosic waste     (wheat straw) for the production of biofuelDesh Bandhu Gangwar
           INTRODUCTIONLignocellulosic waste  material provide world most abundant renewable resource produced through massive photosynthesis.
Cellulosic biomass is an alternative energy feed stock because supplies are abundant domestically and globally.
Ethanol production process only use energy from renewable energy source.The measured residue amount is     approximately 39 millions metric tons of biodegradable cellulose.Pretreatment strategies:-mechanical combination -steam explosion -ammonia fiber explosion-acid and alkali pretreatment-biological treatment
OBJECTIVETo use suitable physical and chemical method for treatment of agro-waste.Bioconversion of agro-waste into fermentable sugars.The production of ethanol.
METHODOLOGYLignocellulosic materialPre - treatmentHydrolysisFermentationEthanol
SEPRATION OF HEMICELLULOSE, LIGNIN AND CELLULOSE COMPONENT OF WHEAT STRAW                                    Wheat straw                                                                                                                                 Steam Treatment                                                                                                                                      Water soluble                                                                                                                                         Insoluble                                 (Hemicellulose)                                                                                                                                                                                                                          (Lignin + Cellulose)                                                                                                                                                                                                                          aq. Alkali Treatment                                                                                                                        soluble                                                                                                          insoluble                                                                                                                                                  (Lignin)                                                                                                                                    Adjust pH                                                                                                                                      7.0                                                                                                                              Lignin                                                                                                      Cellulose
RESULTS1. Percent composition of hemicellulose, lignin and cellulose in wheat straw
2.Effect of high pressure steaming 3.Estimation of total sugar concentration by by (Scott and Melvin, 1953) method.Extract 1 : Raw material treated with 1% acid (Concentrated H2SO4) Extract 2:  Raw material treated with 2% NaOH (alkali)
4. Estimation of total reducing sugar concentration by Miller et al., 1972) method.. 5.Estimation of total celluloseconcentration by    (Updegraph , 1969) method
6.Estimation of total cellulose concentration by (Updegraph, 1969) method7.Estimation of total hemicelluloses concentration by (Bailey,1957) method.
Effect of temperature on ethanol production
Concentration of EthanolEffect of pH on ethanol production(acid treated wheat strawEffect of pH on ethanol production(alkali treated wheat straw)0.250.30.20.25Concentration of Ethanol0.150.2pH 5pH 50.15pH 70.1pH 70.10.050.050012341234DaysDaysEffect of pH on ethanol production
     CONCLUSION      It was concluded that extract 1 i.e. acid treated wheat straw contain maximum amount of total sugar, reducing sugar and xylose concentration in comparison to extract 2. Extract 1 i.e. acid treated extract gave slightly better production of fermentable sugar.

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Desh bandhu gangwar

  • 1. Bioconversion of Lignocellulosic waste (wheat straw) for the production of biofuelDesh Bandhu Gangwar
  • 2. INTRODUCTIONLignocellulosic waste material provide world most abundant renewable resource produced through massive photosynthesis.
  • 3. Cellulosic biomass is an alternative energy feed stock because supplies are abundant domestically and globally.
  • 4. Ethanol production process only use energy from renewable energy source.The measured residue amount is approximately 39 millions metric tons of biodegradable cellulose.Pretreatment strategies:-mechanical combination -steam explosion -ammonia fiber explosion-acid and alkali pretreatment-biological treatment
  • 5. OBJECTIVETo use suitable physical and chemical method for treatment of agro-waste.Bioconversion of agro-waste into fermentable sugars.The production of ethanol.
  • 6. METHODOLOGYLignocellulosic materialPre - treatmentHydrolysisFermentationEthanol
  • 7. SEPRATION OF HEMICELLULOSE, LIGNIN AND CELLULOSE COMPONENT OF WHEAT STRAW Wheat straw Steam Treatment Water soluble Insoluble (Hemicellulose) (Lignin + Cellulose) aq. Alkali Treatment soluble insoluble (Lignin) Adjust pH 7.0 Lignin Cellulose
  • 8. RESULTS1. Percent composition of hemicellulose, lignin and cellulose in wheat straw
  • 9. 2.Effect of high pressure steaming 3.Estimation of total sugar concentration by by (Scott and Melvin, 1953) method.Extract 1 : Raw material treated with 1% acid (Concentrated H2SO4) Extract 2: Raw material treated with 2% NaOH (alkali)
  • 10. 4. Estimation of total reducing sugar concentration by Miller et al., 1972) method.. 5.Estimation of total celluloseconcentration by (Updegraph , 1969) method
  • 11. 6.Estimation of total cellulose concentration by (Updegraph, 1969) method7.Estimation of total hemicelluloses concentration by (Bailey,1957) method.
  • 12. Effect of temperature on ethanol production
  • 13. Concentration of EthanolEffect of pH on ethanol production(acid treated wheat strawEffect of pH on ethanol production(alkali treated wheat straw)0.250.30.20.25Concentration of Ethanol0.150.2pH 5pH 50.15pH 70.1pH 70.10.050.050012341234DaysDaysEffect of pH on ethanol production
  • 14. CONCLUSION It was concluded that extract 1 i.e. acid treated wheat straw contain maximum amount of total sugar, reducing sugar and xylose concentration in comparison to extract 2. Extract 1 i.e. acid treated extract gave slightly better production of fermentable sugar.
  • 15. The best pH was pH- 5 and the best temperature was 300 C for the production of ethanol. Thank You