This study investigated the effects of the organic fertilizer Amina P on pineapple yield and soil microbial populations using a randomized complete block design. Amina P was applied to pineapple plots and inorganic fertilizer was used in other plots. Results found that Amina P increased pineapple growth and yield compared to inorganic fertilizer or no fertilizer. Next generation sequencing also found that Amina P increased beneficial soil microbial populations. The authors recommend Amina P as an environmentally friendly alternative to inorganic fertilizer for organic pineapple farming.