2024 Trend Updates: What Really Works In SEO & Content MarketingSearch Engine Journal
油
The future of SEO is trending toward a more human-first and user-centric approach, powered by AI intelligence and collaboration. Are you ready?
Watch as we explore which SEO trends to prioritize to achieve sustainable growth and deliver reliable results. Well dive into best practices to adapt your strategy around industry-wide disruptions like SGE, how to navigate the top challenges SEO professionals are facing, and proven tactics for prioritizing quality and building trust.
Youll hear:
- The top SEO trends to prioritize in 2024 to achieve long-term success.
- Predictions for SGEs impact, and how to adapt.
- What E-E-A-T really means, and how to implement it holistically (hint: its never been more important).
With Zack Kadish and Alex Carchietta, well show you which SEO trends to ignore and which to focus on, along with the solution to overcoming rapid, significant and disruptive Google algorithm updates.
If youre looking to cut through the noise of constant SEO and content trends to drive success, you wont want to miss this webinar.
Storytelling For The Web: Integrate Storytelling in your Design ProcessChiara Aliotta
油
In this slides I explain how I have used storytelling techniques to elevate websites and brands and create memorable user experiences. You can discover practical tips as I showcase the elements of good storytelling and its applied to some examples of diverse brands/projects..
This presentation by Thibault Schrepel, Associate Professor of Law at Vrije Universiteit Amsterdam University, was made during the discussion Artificial Intelligence, Data and Competition held at the 143rd meeting of the OECD Competition Committee on 12 June 2024. More papers and presentations on the topic can be found at oe.cd/aicomp.
This presentation was uploaded with the authors consent.
How to Leverage AI to Boost Employee Wellness - Lydia Di Francesco - SocialHR...SocialHRCamp
油
Speaker: Lydia Di Francesco
In this workshop, participants will delve into the realm of AI and its profound potential to revolutionize employee wellness initiatives. From stress management to fostering work-life harmony, AI offers a myriad of innovative tools and strategies that can significantly enhance the wellbeing of employees in any organization. Attendees will learn how to effectively leverage AI technologies to cultivate a healthier, happier, and more productive workforce. Whether it's utilizing AI-powered chatbots for mental health support, implementing data analytics to identify internal, systemic risk factors, or deploying personalized wellness apps, this workshop will equip participants with actionable insights and best practices to harness the power of AI for boosting employee wellness. Join us and discover how AI can be a strategic partner towards a culture of wellbeing and resilience in the workplace.
2024 State of Marketing Report by HubspotMarius Sescu
油
https://www.hubspot.com/state-of-marketing
揃 Scaling relationships and proving ROI
揃 Social media is the place for search, sales, and service
揃 Authentic influencer partnerships fuel brand growth
揃 The strongest connections happen via call, click, chat, and camera.
揃 Time saved with AI leads to more creative work
揃 Seeking: A single source of truth
揃 TLDR; Get on social, try AI, and align your systems.
揃 More human marketing, powered by robots
Transgenic sheep are genetically modified to enhance wool quality and yield through transgenesis. By introducing KAP and KIF genes, scientists improve fiber strength, elasticity, and fineness. This innovation revolutionizes wool production, benefiting the textile industry with superior, high-performance fibers.
LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) is a powerful analytical tool for comparing innovator and biosimilar drugs. It ensures precise characterization, detecting structural variations, impurities, and post-translational modifications, ensuring biosimilar quality, efficacy, and regulatory compliance in pharmaceutical development.
Unjustly Incriminating Bacteria: the Role of Bacteriophages in Bacterial Infe...christianagboeze2427
油
SUMMARY
Based on human relationship with bacteria, virulence is one of the most important case to us. Some forms of virulence thought to arise only from the actions of bacteria are not actually caused by them but are indirectly influenced by another counterpart in the microbial mix of the ecosystem called bacteriophage; viruses that only infect prokaryotes such as bacteria but not eukaryotes. Bacteriophages preferably attack bacteria due to the lack of specific receptors for phages on eukaryotic cells which are found in bacteria e.g. peptide sequences and polysaccharide moieties in gram positive and gram negative bacteria, bacterial capsules, slime layers, flagella etc. They recognize and bind to bacteria using appropriate receptors, subsequently proceeding to inject their genome called prophage into their host. This review focuses on the most probable outcomes of phage-host interactions via the lytic and lysogenic cycles which are therapeutic effect and pathogenicity/resistance to antibiotics respectively. By lysogenic conversion or transfer of acquired genetic materials via transduction, phages can confer unusual traits such as virulence and antibiotics resistance. Important pathogenic bacteria that cause persistent and critical infections which have their pathogenicity engineered by phages include Pseudomonas aeruginosa, Salmonella enterica, Escherichia coli, Vibrio cholerae, Staphylococcus spp., and Clostridium spp.
The prophages influence their virulence in a variety of ways which include: contribution to the production of phage-encoded toxins, modification of the bacterial envelope, mediation of bacterial infectivity, and control of bacterial cell regulation. The unwavering threat of antimicrobial resistance in global health, extreme difficulty involved in developing novel antibiotics, and the rate at which microorganisms develop resistance to newly introduced antimicrobials have sparked urgency and interest in research for effective methods to eradicate pathogenic bacteria and limit antibiotic resistance. As a result, interest in phage therapy has been reignited because of the high efficiency in detecting and killing pathogenic bacteria by phages.
Automating Compression Ultrasonography of Human Thigh Tissue and Vessels via ...ThrombUS+ Project
油
Rytis Jurkonis from Kaunas University of Technology (Lithuania) presented their recent work entitled Automating Compression Ultrasonography of Human Thigh Tissue and Vessels via Strain Estimation." Rytis presented on the methodology along the novel wearable hardware developed to automate compression ultrasonography for DVT detection in the lower limbs. In addition, preliminary results were shared, highlighting the feasibility of an operator-independent method to perform compression ultrasonography.
Presented at BIOSTEC 2025 in Porto, Portugal.
About ThrombUS+: Our interdisciplinary approach centers around creating a novel wearable diagnostic device utilizing autonomous, AI-driven DVT detection. This groundbreaking device incorporates wearable ultrasound hardware, impedance plethysmography, and light reflection rheography for early clot detection. ThrombUS+ is designed for postoperative patients, those undergoing lengthy surgical procedures, cancer patients, bedridden individuals at home or in care units, and women during pregnancy and postpartum.
TOP 10 CBSE Top Science Projects for Classes 6 to 10 with Youtube TutorialVivek Bhakta
油
Top 10 CBSE Science Projects for Classes 6 to 10 | Easy DIY Models with YouTube Tutorial
Looking for the best CBSE science projects for Classes 6 to 10? Heres a collection of Top 10 working models that are perfect for science exhibitions, school projects, and STEM learning. These projects cover essential science concepts from physics, chemistry, and biology, making them both fun and educational.
Each project includes a step-by-step YouTube tutorial, so students can easily follow along and build their own models.
Top 10 CBSE Science Projects for Classes 6 to 10:
1鏝 Hydraulic Bridge Model Demonstrate the principles of hydraulics and Pascals Law.
2鏝 Electric Motor Model Understand how electromagnetism powers motors.
3鏝 Solar-Powered Car Explore renewable energy and motion mechanics.
4鏝 Wind Turbine Generator Convert wind energy into electrical power.
5鏝 Automatic Street Light System Learn about LDR sensors and energy efficiency.
6鏝 Water Dispenser Model Show the role of air pressure in fluid movement.
7鏝 Earthquake Alarm System Build a vibration-based alert system for disaster safety.
8鏝 Biogas Plant Model Explain how organic waste is converted into energy.
9鏝 Rainwater Harvesting Model Demonstrate sustainable water conservation techniques.
Smart Irrigation System Create an automated plant watering system using sensors.
Why Choose These Projects?
Simple & Fun Uses easily available materials.
Educational & Practical Covers key CBSE science topics.
YouTube Video Guide Step-by-step tutorials for easy learning.
Watch the full YouTube tutorial and start building your project today!
Energ and Energy Forms, Work, and Power | IGCSE PhysicsBlessing Ndazie
油
This extensive slide deck provides a detailed exploration of energy, work, and power for IGCSE Physics. It covers fundamental concepts such as the definition of work done, kinetic energy, potential energy, mechanical energy, conservation of energy, efficiency, and power. The presentation also includes energy transfer, renewable and non-renewable energy sources, calculation of work done, power output, and real-life applications of energy principles. Featuring illustrative diagrams, worked examples, and exam-style questions, this resource is ideal for IGCSE students, teachers, and independent learners preparing for exams.
Cell division is a fundamental biological process that enables the growth, development, and repair of living organisms. It's the process by which a parent cell divides into two or more daughter cells, each carrying a complete set of genetic instructions. This intricate process occurs in two primary ways: mitosis and meiosis. Mitosis is responsible for the creation of identical daughter cells, ensuring the maintenance of genetic information for growth and tissue repair. Meiosis, on the other hand, is a specialized form of cell division that occurs in sexually reproducing organisms, producing gametes (sperm and egg cells) with half the number of chromosomes, contributing to genetic diversity in offspring.
Difference between Prokaryotic cell and Eukaryotic cell.pptxDrSulabhaDeokar
油
This presentation explores the fundamental differences between prokaryotic and eukaryotic cells,distinguishing characteristics of Prokaryotes and Eukaryotes.Describe common cell morphologies and cellular arrangements in typical Prokaryotes and Eukaryotes.
Presentation explains how cells maintain their morphology.
Explore internal and external structures of Prokaryotes and Eukaryotes in terms of their physical structure, chemical structure and function.
This presentation is designed for biology students, educators, and anyone interested in cellular biology. Based on the latest research and scientific discoveries in the field of Microbiology, Microbial Biotechnology and cellular biology. This Presentation has been compiled using information from trusted educational resources and scientific literature.
The purpose of this presentation is to educate and inform the students about the fundamental differences between prokaryotic and eukaryotic cells, highlighting their unique structures, functions, and characteristics, which provide a comprehensive understanding of cellular biology.
Educate the students and teachers about the differences between prokaryotic and eukaryotic cells in detail.
This presentation will engage and entertain the students, often with a mix of detail information, colourful pictures and storytelling.
This presentation will motivate and inspire the students to think differently, take action, or pursue a goal.
Definitely it will raise awareness about a ultrastructures of Prokaryotes and Eukaryotes .
This presentation will Provide an update or report on a science projects and progress as well as inspire the graduate students to learn more about cellular biology and its applications.
This presentation will inspire studets, teachers and educational professionals to explore digital resource for e - learnig .
Presentation likely to be used by under graduate and post graduate students, educators or individuals for online learning.
It can work as digital resource for a broader e- learning ecosystem.
This presentation highlights '' NEP-aligned Biotechnology and Biology education.''
"Discover the distinctions between prokaryotic and eukaryotic cells, from cell walls to genetic material. This presentation provides a comprehensive overview of cellular biology.Learn about the two main types of cells - prokaryotic,eukaryotic and their differences in structure, function, and organization. A great resource for biology learners.Uncover the unique characteristics of prokaryotic and eukaryotic cells in this informative PPT.
SILICON IS AN INHIBITOR OF CERTAIN ENZYMES IN VITROLilya BOUCELHA
油
Silicon is considered an inorganic biostimulant and a prophylactic extracellular agent that allows the stimulation of a
wide range of natural defences against abiotic and biotic stresses. However, little or no work has focused on the direct action of silicon on some enzymes. Indeed, during this study, the action of silicon was studied in vitro by direct contact of this element at different doses with the enzymatic extracts of Trigonella foenum-graecum L. (fenugreek) seeds. Our results showed that silicon
strongly inhibited antioxidant and hydrolytic enzymatic activities. The percentage of this inhibition depends on the dose of silicon and the type of enzyme. The most sensitive enzymes to this inhibition were SOD and lipases whose activity was totally inhibited at
4 mM and 7 mM respectively. However, we report that the inhibitory action of silicon was limited to 50% for GPOX whatever the concentration of silicon used, the plateau being reached at 10 mM for GPOX and at 70 mM for proteases. Since these enzymes are mainly metallo-dependent, we hypothesize that their inhibition by silicon may be due to interactions between silicon and the metals involved in the functioning of each enzyme. Our study shows that silicon can be used as an inhibitor of enzymes involved in certain diseases.
Variation and Natural Selection | IGCSE BiologyBlessing Ndazie
油
This extensive slide deck provides a detailed exploration of variation and natural selection for IGCSE Biology. It covers key concepts such as genetic and environmental variation, types of variation (continuous and discontinuous), mutation, evolution, and the principles of natural selection. The presentation also explains Darwins theory of evolution, adaptation, survival of the fittest, selective breeding, antibiotic resistance in bacteria, and speciation. With illustrative diagrams, real-life examples, and exam-style questions, this resource is ideal for IGCSE students, teachers, and independent learners preparing for exams.
Wepresent the localizationandhostgalaxyofFRB20190208A, arepeatingsourceof fast radiobursts (FRBs) discoveredusingCHIME/FRB.Aspartof thePinpointingREpeatingChImeSourceswithEVNdishesrepeater localizationprogramon theEuropeanVLBINetwork (EVN),wemonitoredFRB20190208Afor 65.6hr at 1.4GHzanddetectedasingleburst,whichledtoitsverylongbaselineinterferometrylocalizationwith260mas uncertainty(2).Follow-upopticalobservationswiththeMMTObservatory(i25.7mag(AB))foundnovisible hostattheFRBposition.SubsequentdeeperobservationswiththeGranTelescopioCanarias,however,revealedan extremelyfaintgalaxy(r=27.32賊0.16mag),verylikely(99.95%)associatedwithFRB20190208A.Giventhe dispersionmeasureoftheFRB(580pccm3),eventhemostconservativeredshiftestimate( ~ z 0.83 max )implies TheAstrophysicalJournalLetters,977:L4(17pp),2024December10 https://doi.org/10.3847/2041-8213/ad8ce1 息2024.TheAuthor(s).PublishedbytheAmericanAstronomicalSociety. 30BantingFellow. 31McGillSpaceInstituteFellow. 32 FRQNTPostdoctoralFellow. Originalcontent fromthisworkmaybeusedunder theterms of theCreativeCommonsAttribution4.0licence.Anyfurther distributionofthisworkmustmaintainattributiontotheauthor(s)andthetitle of thework, journalcitationandDOI. 1The Astrophysical Journal Letters, 977:L4 (17pp), 2024 December 10 Hewitt et al. that this is the lowest-luminosity FRB host to date (108 Le), even less luminous than the dwarf host of FRB20121102A. We investigate how localization precision and the depth of optical imaging affect host association and discuss the implications of such a low-luminosity dwarf galaxy. Unlike the other repeaters with low-luminosity hosts, FRB 20190208A has a modest Faraday rotation measure of a few tens of rad m2, and EVN plus Very Large Array observations reveal no associated compact persistent radio source. We also monitored FRB20190208A for 40.4hr over 2yr as part of the Extragalactic Coherent Light from Astrophysical Transients repeating FRB monitoring campaign on the Nan巽ay Radio Telescope and detected one burst. Our results demonstrate that, in some cases, the robust association of an FRB with a host galaxy will require both high localization precision and deep optical follow-up. Unified Astronomy Thesaurus concepts: Radio bursts (1339); Radio transient sources (2008); Very long baseline interferometry (1769); Dwarf galaxies (416)
Pig farming, pork farming, pig production or hog farming is the raising and breeding of domestic pigs as livestock, and is a branch of animal husbandry. Pigs are farmed principally for food (e.g. pork: bacon, ham, gammon) and skins.
Pigs are amenable to many different styles of farming: intensive commercial units, commercial free range enterprises, or extensive farming (being allowed to wander around a village, town or city, or tethered in a simple shelter or kept in a pen outside the owner's house). Historically, farm pigs were kept in small numbers and were closely associated with the residence of the owner, or in the same village or town.[1] They were valued as a source of meat and fat, and for their ability to convert inedible food into meat and manure, and were often fed household food waste when kept on a homestead.[2] Pigs have been farmed to dispose of municipal garbage on a large scale.[3]
All these forms of pig farm are in use today, though intensive farms are by far the most popular, due to their potential to raise a large amount of pigs in a very cost-efficient manner.[4] In developed nations, commercial farms house thousands of pigs in climate-controlled buildings.[5] Pigs are a popular form of livestock, with more than one billion pigs butchered each year worldwide, 100 million in the United States. The majority of pigs are used for human food, but also supply skin, fat and other materials for use in clothing, ingredients for processed foods,[6] cosmetics,[7] and medical use.[8]Pig farming has gained importance today. Pigs have inherited capacity to acclimatize with varying climatic conditions. Pigs cannot withstand high temperature climate.
Pigs are adjusted to varied rearing practices and consume different types of food (Omnivorous) to attain higher growth and meat production.
Pigs will attain 60-70kg body weight in 6-8months period.
Female pigs i.e., sows will come to heat at age of 8-9 months but avoid using male pigs (Boars) for breeding purpose until it attains one year of age.
Adult sows when bred during right time after attaining maturity will farrow 8-12 piglets in 112-118 days of gestation period (i.e., about 4 months of gestation). Feedefficiencyis to gain one Kg live weightfor every 2.75-3kg feed consumed (FCR: 1:2.75). There are many advantageous in pig rearing. Pork is available at a cheaper price with nutritious and highly palatable tasty meat of higher quality animal protein. Pig bones are used for producing bone meal and also used for purification of sugar in sugar industry.
The manure droppings and urine are good fertilizers which enhance the soil fertilityand improve grain production.
Pig hairs (Bristles) are used for making brushes and ropes, hooves are used for shirt button making and preparation of gum. Hence, pigs are called as multi utility domestic animals. Farmers can take up piggery farming and reduce their debt burden and improve their profits and livelihood.
Simple Phenomena of Magnetism | IGCSE PhysicsBlessing Ndazie
油
This extensive slide deck provides a detailed exploration of the simple phenomena of magnetism for IGCSE Physics. It covers key concepts such as magnetic materials, properties of magnets, magnetic field patterns, the Earth's magnetism, electromagnets, the motor effect, and the principles of electromagnetic induction. The presentation also explains magnetization and demagnetization, methods of making magnets, applications of magnets in real life, and experimental demonstrations. Featuring illustrative diagrams, worked examples, and exam-style questions, this resource is ideal for IGCSE students, teachers, and independent learners preparing for exams.
Deep Learning-Driven Protein Design for Maize Improvement: AI-Guided Solution...Muhammad Salman Iqbal
油
Bridging AI, Synthetic Biology, and Crop Science to Address Global Food Security.
This presentation explores the transformative potential of AI-driven protein design in revolutionizing maize (corn) breeding. Learn how deep learning models like AlphaFold, ESMFold, and RFdiffusion enable rapid engineering of stress-resilient proteins for:
Disease resistance (e.g., fungal pathogens like Fusarium and Puccinia)
Drought and heat tolerance (synthetic transcription factors for root and stomatal optimization)
Nutrient efficiency (engineered phosphate/nitrogen transporters)
Enhanced photosynthesis (AI-designed carbonic anhydrases)
Key highlights:
Case studies from Cell, Science, and Nature Biotechnology (20232024) showcasing AI-designed proteins validated in field trials.
Ethical considerations and future directions for AI-guided CRISPR integration in crop improvement.
Visual summaries of protein structures, field data, and AI workflows.
Target audience: Plant scientists, agronomists, bioinformaticians, AI researchers, and students in biotechnology and agriculture.
Hashtags:
#DeepLearning #ProteinDesign #MaizeImprovement #AIinAgriculture #SustainableFarming #CropBreeding #SyntheticBiology #FoodSecurity #AlphaFold #CRISPR
ChatGPT is a revolutionary addition to the world since its introduction in 2022. A big shift in the sector of information gathering and processing happened because of this chatbot. What is the story of ChatGPT? How is the bot responding to prompts and generating contents? Swipe through these slides prepared by Expeed Software, a web development company regarding the development and technical intricacies of ChatGPT!
Product Design Trends in 2024 | Teenage EngineeringsPixeldarts
油
The realm of product design is a constantly changing environment where technology and style intersect. Every year introduces fresh challenges and exciting trends that mold the future of this captivating art form. In this piece, we delve into the significant trends set to influence the look and functionality of product design in the year 2024.
Transgenic sheep are genetically modified to enhance wool quality and yield through transgenesis. By introducing KAP and KIF genes, scientists improve fiber strength, elasticity, and fineness. This innovation revolutionizes wool production, benefiting the textile industry with superior, high-performance fibers.
LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry) is a powerful analytical tool for comparing innovator and biosimilar drugs. It ensures precise characterization, detecting structural variations, impurities, and post-translational modifications, ensuring biosimilar quality, efficacy, and regulatory compliance in pharmaceutical development.
Unjustly Incriminating Bacteria: the Role of Bacteriophages in Bacterial Infe...christianagboeze2427
油
SUMMARY
Based on human relationship with bacteria, virulence is one of the most important case to us. Some forms of virulence thought to arise only from the actions of bacteria are not actually caused by them but are indirectly influenced by another counterpart in the microbial mix of the ecosystem called bacteriophage; viruses that only infect prokaryotes such as bacteria but not eukaryotes. Bacteriophages preferably attack bacteria due to the lack of specific receptors for phages on eukaryotic cells which are found in bacteria e.g. peptide sequences and polysaccharide moieties in gram positive and gram negative bacteria, bacterial capsules, slime layers, flagella etc. They recognize and bind to bacteria using appropriate receptors, subsequently proceeding to inject their genome called prophage into their host. This review focuses on the most probable outcomes of phage-host interactions via the lytic and lysogenic cycles which are therapeutic effect and pathogenicity/resistance to antibiotics respectively. By lysogenic conversion or transfer of acquired genetic materials via transduction, phages can confer unusual traits such as virulence and antibiotics resistance. Important pathogenic bacteria that cause persistent and critical infections which have their pathogenicity engineered by phages include Pseudomonas aeruginosa, Salmonella enterica, Escherichia coli, Vibrio cholerae, Staphylococcus spp., and Clostridium spp.
The prophages influence their virulence in a variety of ways which include: contribution to the production of phage-encoded toxins, modification of the bacterial envelope, mediation of bacterial infectivity, and control of bacterial cell regulation. The unwavering threat of antimicrobial resistance in global health, extreme difficulty involved in developing novel antibiotics, and the rate at which microorganisms develop resistance to newly introduced antimicrobials have sparked urgency and interest in research for effective methods to eradicate pathogenic bacteria and limit antibiotic resistance. As a result, interest in phage therapy has been reignited because of the high efficiency in detecting and killing pathogenic bacteria by phages.
Automating Compression Ultrasonography of Human Thigh Tissue and Vessels via ...ThrombUS+ Project
油
Rytis Jurkonis from Kaunas University of Technology (Lithuania) presented their recent work entitled Automating Compression Ultrasonography of Human Thigh Tissue and Vessels via Strain Estimation." Rytis presented on the methodology along the novel wearable hardware developed to automate compression ultrasonography for DVT detection in the lower limbs. In addition, preliminary results were shared, highlighting the feasibility of an operator-independent method to perform compression ultrasonography.
Presented at BIOSTEC 2025 in Porto, Portugal.
About ThrombUS+: Our interdisciplinary approach centers around creating a novel wearable diagnostic device utilizing autonomous, AI-driven DVT detection. This groundbreaking device incorporates wearable ultrasound hardware, impedance plethysmography, and light reflection rheography for early clot detection. ThrombUS+ is designed for postoperative patients, those undergoing lengthy surgical procedures, cancer patients, bedridden individuals at home or in care units, and women during pregnancy and postpartum.
TOP 10 CBSE Top Science Projects for Classes 6 to 10 with Youtube TutorialVivek Bhakta
油
Top 10 CBSE Science Projects for Classes 6 to 10 | Easy DIY Models with YouTube Tutorial
Looking for the best CBSE science projects for Classes 6 to 10? Heres a collection of Top 10 working models that are perfect for science exhibitions, school projects, and STEM learning. These projects cover essential science concepts from physics, chemistry, and biology, making them both fun and educational.
Each project includes a step-by-step YouTube tutorial, so students can easily follow along and build their own models.
Top 10 CBSE Science Projects for Classes 6 to 10:
1鏝 Hydraulic Bridge Model Demonstrate the principles of hydraulics and Pascals Law.
2鏝 Electric Motor Model Understand how electromagnetism powers motors.
3鏝 Solar-Powered Car Explore renewable energy and motion mechanics.
4鏝 Wind Turbine Generator Convert wind energy into electrical power.
5鏝 Automatic Street Light System Learn about LDR sensors and energy efficiency.
6鏝 Water Dispenser Model Show the role of air pressure in fluid movement.
7鏝 Earthquake Alarm System Build a vibration-based alert system for disaster safety.
8鏝 Biogas Plant Model Explain how organic waste is converted into energy.
9鏝 Rainwater Harvesting Model Demonstrate sustainable water conservation techniques.
Smart Irrigation System Create an automated plant watering system using sensors.
Why Choose These Projects?
Simple & Fun Uses easily available materials.
Educational & Practical Covers key CBSE science topics.
YouTube Video Guide Step-by-step tutorials for easy learning.
Watch the full YouTube tutorial and start building your project today!
Energ and Energy Forms, Work, and Power | IGCSE PhysicsBlessing Ndazie
油
This extensive slide deck provides a detailed exploration of energy, work, and power for IGCSE Physics. It covers fundamental concepts such as the definition of work done, kinetic energy, potential energy, mechanical energy, conservation of energy, efficiency, and power. The presentation also includes energy transfer, renewable and non-renewable energy sources, calculation of work done, power output, and real-life applications of energy principles. Featuring illustrative diagrams, worked examples, and exam-style questions, this resource is ideal for IGCSE students, teachers, and independent learners preparing for exams.
Cell division is a fundamental biological process that enables the growth, development, and repair of living organisms. It's the process by which a parent cell divides into two or more daughter cells, each carrying a complete set of genetic instructions. This intricate process occurs in two primary ways: mitosis and meiosis. Mitosis is responsible for the creation of identical daughter cells, ensuring the maintenance of genetic information for growth and tissue repair. Meiosis, on the other hand, is a specialized form of cell division that occurs in sexually reproducing organisms, producing gametes (sperm and egg cells) with half the number of chromosomes, contributing to genetic diversity in offspring.
Difference between Prokaryotic cell and Eukaryotic cell.pptxDrSulabhaDeokar
油
This presentation explores the fundamental differences between prokaryotic and eukaryotic cells,distinguishing characteristics of Prokaryotes and Eukaryotes.Describe common cell morphologies and cellular arrangements in typical Prokaryotes and Eukaryotes.
Presentation explains how cells maintain their morphology.
Explore internal and external structures of Prokaryotes and Eukaryotes in terms of their physical structure, chemical structure and function.
This presentation is designed for biology students, educators, and anyone interested in cellular biology. Based on the latest research and scientific discoveries in the field of Microbiology, Microbial Biotechnology and cellular biology. This Presentation has been compiled using information from trusted educational resources and scientific literature.
The purpose of this presentation is to educate and inform the students about the fundamental differences between prokaryotic and eukaryotic cells, highlighting their unique structures, functions, and characteristics, which provide a comprehensive understanding of cellular biology.
Educate the students and teachers about the differences between prokaryotic and eukaryotic cells in detail.
This presentation will engage and entertain the students, often with a mix of detail information, colourful pictures and storytelling.
This presentation will motivate and inspire the students to think differently, take action, or pursue a goal.
Definitely it will raise awareness about a ultrastructures of Prokaryotes and Eukaryotes .
This presentation will Provide an update or report on a science projects and progress as well as inspire the graduate students to learn more about cellular biology and its applications.
This presentation will inspire studets, teachers and educational professionals to explore digital resource for e - learnig .
Presentation likely to be used by under graduate and post graduate students, educators or individuals for online learning.
It can work as digital resource for a broader e- learning ecosystem.
This presentation highlights '' NEP-aligned Biotechnology and Biology education.''
"Discover the distinctions between prokaryotic and eukaryotic cells, from cell walls to genetic material. This presentation provides a comprehensive overview of cellular biology.Learn about the two main types of cells - prokaryotic,eukaryotic and their differences in structure, function, and organization. A great resource for biology learners.Uncover the unique characteristics of prokaryotic and eukaryotic cells in this informative PPT.
SILICON IS AN INHIBITOR OF CERTAIN ENZYMES IN VITROLilya BOUCELHA
油
Silicon is considered an inorganic biostimulant and a prophylactic extracellular agent that allows the stimulation of a
wide range of natural defences against abiotic and biotic stresses. However, little or no work has focused on the direct action of silicon on some enzymes. Indeed, during this study, the action of silicon was studied in vitro by direct contact of this element at different doses with the enzymatic extracts of Trigonella foenum-graecum L. (fenugreek) seeds. Our results showed that silicon
strongly inhibited antioxidant and hydrolytic enzymatic activities. The percentage of this inhibition depends on the dose of silicon and the type of enzyme. The most sensitive enzymes to this inhibition were SOD and lipases whose activity was totally inhibited at
4 mM and 7 mM respectively. However, we report that the inhibitory action of silicon was limited to 50% for GPOX whatever the concentration of silicon used, the plateau being reached at 10 mM for GPOX and at 70 mM for proteases. Since these enzymes are mainly metallo-dependent, we hypothesize that their inhibition by silicon may be due to interactions between silicon and the metals involved in the functioning of each enzyme. Our study shows that silicon can be used as an inhibitor of enzymes involved in certain diseases.
Variation and Natural Selection | IGCSE BiologyBlessing Ndazie
油
This extensive slide deck provides a detailed exploration of variation and natural selection for IGCSE Biology. It covers key concepts such as genetic and environmental variation, types of variation (continuous and discontinuous), mutation, evolution, and the principles of natural selection. The presentation also explains Darwins theory of evolution, adaptation, survival of the fittest, selective breeding, antibiotic resistance in bacteria, and speciation. With illustrative diagrams, real-life examples, and exam-style questions, this resource is ideal for IGCSE students, teachers, and independent learners preparing for exams.
Wepresent the localizationandhostgalaxyofFRB20190208A, arepeatingsourceof fast radiobursts (FRBs) discoveredusingCHIME/FRB.Aspartof thePinpointingREpeatingChImeSourceswithEVNdishesrepeater localizationprogramon theEuropeanVLBINetwork (EVN),wemonitoredFRB20190208Afor 65.6hr at 1.4GHzanddetectedasingleburst,whichledtoitsverylongbaselineinterferometrylocalizationwith260mas uncertainty(2).Follow-upopticalobservationswiththeMMTObservatory(i25.7mag(AB))foundnovisible hostattheFRBposition.SubsequentdeeperobservationswiththeGranTelescopioCanarias,however,revealedan extremelyfaintgalaxy(r=27.32賊0.16mag),verylikely(99.95%)associatedwithFRB20190208A.Giventhe dispersionmeasureoftheFRB(580pccm3),eventhemostconservativeredshiftestimate( ~ z 0.83 max )implies TheAstrophysicalJournalLetters,977:L4(17pp),2024December10 https://doi.org/10.3847/2041-8213/ad8ce1 息2024.TheAuthor(s).PublishedbytheAmericanAstronomicalSociety. 30BantingFellow. 31McGillSpaceInstituteFellow. 32 FRQNTPostdoctoralFellow. Originalcontent fromthisworkmaybeusedunder theterms of theCreativeCommonsAttribution4.0licence.Anyfurther distributionofthisworkmustmaintainattributiontotheauthor(s)andthetitle of thework, journalcitationandDOI. 1The Astrophysical Journal Letters, 977:L4 (17pp), 2024 December 10 Hewitt et al. that this is the lowest-luminosity FRB host to date (108 Le), even less luminous than the dwarf host of FRB20121102A. We investigate how localization precision and the depth of optical imaging affect host association and discuss the implications of such a low-luminosity dwarf galaxy. Unlike the other repeaters with low-luminosity hosts, FRB 20190208A has a modest Faraday rotation measure of a few tens of rad m2, and EVN plus Very Large Array observations reveal no associated compact persistent radio source. We also monitored FRB20190208A for 40.4hr over 2yr as part of the Extragalactic Coherent Light from Astrophysical Transients repeating FRB monitoring campaign on the Nan巽ay Radio Telescope and detected one burst. Our results demonstrate that, in some cases, the robust association of an FRB with a host galaxy will require both high localization precision and deep optical follow-up. Unified Astronomy Thesaurus concepts: Radio bursts (1339); Radio transient sources (2008); Very long baseline interferometry (1769); Dwarf galaxies (416)
Pig farming, pork farming, pig production or hog farming is the raising and breeding of domestic pigs as livestock, and is a branch of animal husbandry. Pigs are farmed principally for food (e.g. pork: bacon, ham, gammon) and skins.
Pigs are amenable to many different styles of farming: intensive commercial units, commercial free range enterprises, or extensive farming (being allowed to wander around a village, town or city, or tethered in a simple shelter or kept in a pen outside the owner's house). Historically, farm pigs were kept in small numbers and were closely associated with the residence of the owner, or in the same village or town.[1] They were valued as a source of meat and fat, and for their ability to convert inedible food into meat and manure, and were often fed household food waste when kept on a homestead.[2] Pigs have been farmed to dispose of municipal garbage on a large scale.[3]
All these forms of pig farm are in use today, though intensive farms are by far the most popular, due to their potential to raise a large amount of pigs in a very cost-efficient manner.[4] In developed nations, commercial farms house thousands of pigs in climate-controlled buildings.[5] Pigs are a popular form of livestock, with more than one billion pigs butchered each year worldwide, 100 million in the United States. The majority of pigs are used for human food, but also supply skin, fat and other materials for use in clothing, ingredients for processed foods,[6] cosmetics,[7] and medical use.[8]Pig farming has gained importance today. Pigs have inherited capacity to acclimatize with varying climatic conditions. Pigs cannot withstand high temperature climate.
Pigs are adjusted to varied rearing practices and consume different types of food (Omnivorous) to attain higher growth and meat production.
Pigs will attain 60-70kg body weight in 6-8months period.
Female pigs i.e., sows will come to heat at age of 8-9 months but avoid using male pigs (Boars) for breeding purpose until it attains one year of age.
Adult sows when bred during right time after attaining maturity will farrow 8-12 piglets in 112-118 days of gestation period (i.e., about 4 months of gestation). Feedefficiencyis to gain one Kg live weightfor every 2.75-3kg feed consumed (FCR: 1:2.75). There are many advantageous in pig rearing. Pork is available at a cheaper price with nutritious and highly palatable tasty meat of higher quality animal protein. Pig bones are used for producing bone meal and also used for purification of sugar in sugar industry.
The manure droppings and urine are good fertilizers which enhance the soil fertilityand improve grain production.
Pig hairs (Bristles) are used for making brushes and ropes, hooves are used for shirt button making and preparation of gum. Hence, pigs are called as multi utility domestic animals. Farmers can take up piggery farming and reduce their debt burden and improve their profits and livelihood.
Simple Phenomena of Magnetism | IGCSE PhysicsBlessing Ndazie
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This extensive slide deck provides a detailed exploration of the simple phenomena of magnetism for IGCSE Physics. It covers key concepts such as magnetic materials, properties of magnets, magnetic field patterns, the Earth's magnetism, electromagnets, the motor effect, and the principles of electromagnetic induction. The presentation also explains magnetization and demagnetization, methods of making magnets, applications of magnets in real life, and experimental demonstrations. Featuring illustrative diagrams, worked examples, and exam-style questions, this resource is ideal for IGCSE students, teachers, and independent learners preparing for exams.
Deep Learning-Driven Protein Design for Maize Improvement: AI-Guided Solution...Muhammad Salman Iqbal
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Bridging AI, Synthetic Biology, and Crop Science to Address Global Food Security.
This presentation explores the transformative potential of AI-driven protein design in revolutionizing maize (corn) breeding. Learn how deep learning models like AlphaFold, ESMFold, and RFdiffusion enable rapid engineering of stress-resilient proteins for:
Disease resistance (e.g., fungal pathogens like Fusarium and Puccinia)
Drought and heat tolerance (synthetic transcription factors for root and stomatal optimization)
Nutrient efficiency (engineered phosphate/nitrogen transporters)
Enhanced photosynthesis (AI-designed carbonic anhydrases)
Key highlights:
Case studies from Cell, Science, and Nature Biotechnology (20232024) showcasing AI-designed proteins validated in field trials.
Ethical considerations and future directions for AI-guided CRISPR integration in crop improvement.
Visual summaries of protein structures, field data, and AI workflows.
Target audience: Plant scientists, agronomists, bioinformaticians, AI researchers, and students in biotechnology and agriculture.
Hashtags:
#DeepLearning #ProteinDesign #MaizeImprovement #AIinAgriculture #SustainableFarming #CropBreeding #SyntheticBiology #FoodSecurity #AlphaFold #CRISPR
ChatGPT is a revolutionary addition to the world since its introduction in 2022. A big shift in the sector of information gathering and processing happened because of this chatbot. What is the story of ChatGPT? How is the bot responding to prompts and generating contents? Swipe through these slides prepared by Expeed Software, a web development company regarding the development and technical intricacies of ChatGPT!
Product Design Trends in 2024 | Teenage EngineeringsPixeldarts
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The realm of product design is a constantly changing environment where technology and style intersect. Every year introduces fresh challenges and exciting trends that mold the future of this captivating art form. In this piece, we delve into the significant trends set to influence the look and functionality of product design in the year 2024.
How Race, Age and Gender Shape Attitudes Towards Mental HealthThinkNow
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Mental health has been in the news quite a bit lately. Dozens of U.S. states are currently suing Meta for contributing to the youth mental health crisis by inserting addictive features into their products, while the U.S. Surgeon General is touring the nation to bring awareness to the growing epidemic of loneliness and isolation. The country has endured periods of low national morale, such as in the 1970s when high inflation and the energy crisis worsened public sentiment following the Vietnam War. The current mood, however, feels different. Gallup recently reported that national mental health is at an all-time low, with few bright spots to lift spirits.
To better understand how Americans are feeling and their attitudes towards mental health in general, ThinkNow conducted a nationally representative quantitative survey of 1,500 respondents and found some interesting differences among ethnic, age and gender groups.
Technology
For example, 52% agree that technology and social media have a negative impact on mental health, but when broken out by race, 61% of Whites felt technology had a negative effect, and only 48% of Hispanics thought it did.
While technology has helped us keep in touch with friends and family in faraway places, it appears to have degraded our ability to connect in person. Staying connected online is a double-edged sword since the same news feed that brings us pictures of the grandkids and fluffy kittens also feeds us news about the wars in Israel and Ukraine, the dysfunction in Washington, the latest mass shooting and the climate crisis.
Hispanics may have a built-in defense against the isolation technology breeds, owing to their large, multigenerational households, strong social support systems, and tendency to use social media to stay connected with relatives abroad.
Age and Gender
When asked how individuals rate their mental health, men rate it higher than women by 11 percentage points, and Baby Boomers rank it highest at 83%, saying its good or excellent vs. 57% of Gen Z saying the same.
Gen Z spends the most amount of time on social media, so the notion that social media negatively affects mental health appears to be correlated. Unfortunately, Gen Z is also the generation thats least comfortable discussing mental health concerns with healthcare professionals. Only 40% of them state theyre comfortable discussing their issues with a professional compared to 60% of Millennials and 65% of Boomers.
Race Affects Attitudes
As seen in previous research conducted by ThinkNow, Asian Americans lag other groups when it comes to awareness of mental health issues. Twenty-four percent of Asian Americans believe that having a mental health issue is a sign of weakness compared to the 16% average for all groups. Asians are also considerably less likely to be aware of mental health services in their communities (42% vs. 55%) and most likely to seek out information on social media (51% vs. 35%).
AI Trends in Creative Operations 2024 by Artwork Flow.pdfmarketingartwork
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Creative operations teams expect increased AI use in 2024. Currently, over half of tasks are not AI-enabled, but this is expected to decrease in the coming year. ChatGPT is the most popular AI tool currently. Business leaders are more actively exploring AI benefits than individual contributors. Most respondents do not believe AI will impact workforce size in 2024. However, some inhibitions still exist around AI accuracy and lack of understanding. Creatives primarily want to use AI to save time on mundane tasks and boost productivity.
Organizational culture includes values, norms, systems, symbols, language, assumptions, beliefs, and habits that influence employee behaviors and how people interpret those behaviors. It is important because culture can help or hinder a company's success. Some key aspects of Netflix's culture that help it achieve results include hiring smartly so every position has stars, focusing on attitude over just aptitude, and having a strict policy against peacocks, whiners, and jerks.
PEPSICO Presentation to CAGNY Conference Feb 2024Neil Kimberley
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PepsiCo provided a safe harbor statement noting that any forward-looking statements are based on currently available information and are subject to risks and uncertainties. It also provided information on non-GAAP measures and directing readers to its website for disclosure and reconciliation. The document then discussed PepsiCo's business overview, including that it is a global beverage and convenient food company with iconic brands, $91 billion in net revenue in 2023, and nearly $14 billion in core operating profit. It operates through a divisional structure with a focus on local consumers.
Content Methodology: A Best Practices Report (Webinar)contently
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This document provides an overview of content methodology best practices. It defines content methodology as establishing objectives, KPIs, and a culture of continuous learning and iteration. An effective methodology focuses on connecting with audiences, creating optimal content, and optimizing processes. It also discusses why a methodology is needed due to the competitive landscape, proliferation of channels, and opportunities for improvement. Components of an effective methodology include defining objectives and KPIs, audience analysis, identifying opportunities, and evaluating resources. The document concludes with recommendations around creating a content plan, testing and optimizing content over 90 days.
How to Prepare For a Successful Job Search for 2024Albert Qian
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The document provides guidance on preparing a job search for 2024. It discusses the state of the job market, focusing on growth in AI and healthcare but also continued layoffs. It recommends figuring out what you want to do by researching interests and skills, then conducting informational interviews. The job search should involve building a personal brand on LinkedIn, actively applying to jobs, tailoring resumes and interviews, maintaining job hunting as a habit, and continuing self-improvement. Once hired, the document advises setting new goals and keeping skills and networking active in case of future opportunities.
A report by thenetworkone and Kurio.
The contributing experts and agencies are (in an alphabetical order): Sylwia Rytel, Social Media Supervisor, 180heartbeats + JUNG v MATT (PL), Sharlene Jenner, Vice President - Director of Engagement Strategy, Abelson Taylor (USA), Alex Casanovas, Digital Director, Atrevia (ES), Dora Beilin, Senior Social Strategist, Barrett Hoffher (USA), Min Seo, Campaign Director, Brand New Agency (KR), Desh辿 M. Gully, Associate Strategist, Day One Agency (USA), Francesca Trevisan, Strategist, Different (IT), Trevor Crossman, CX and Digital Transformation Director; Olivia Hussey, Strategic Planner; Simi Srinarula, Social Media Manager, The Hallway (AUS), James Hebbert, Managing Director, Hylink (CN / UK), Mundy lvarez, Planning Director; Pedro Rojas, Social Media Manager; Pancho Gonz叩lez, CCO, Inbrax (CH), Oana Oprea, Head of Digital Planning, Jam Session Agency (RO), Amy Bottrill, Social Account Director, Launch (UK), Gaby Arriaga, Founder, Leonardo1452 (MX), Shantesh S Row, Creative Director, Liwa (UAE), Rajesh Mehta, Chief Strategy Officer; Dhruv Gaur, Digital Planning Lead; Leonie Mergulhao, Account Supervisor - Social Media & PR, Medulla (IN), Aurelija Plioplyt, Head of Digital & Social, Not Perfect (LI), Daiana Khaidargaliyeva, Account Manager, Osaka Labs (UK / USA), Stefanie Sohnchen, Vice President Digital, PIABO Communications (DE), Elisabeth Winiartati, Managing Consultant, Head of Global Integrated Communications; Lydia Aprina, Account Manager, Integrated Marketing and Communications; Nita Prabowo, Account Manager, Integrated Marketing and Communications; Okhi, Web Developer, PNTR Group (ID), Kei Obusan, Insights Director; Daffi Ranandi, Insights Manager, Radarr (SG), Gautam Reghunath, Co-founder & CEO, Talented (IN), Donagh Humphreys, Head of Social and Digital Innovation, THINKHOUSE (IRE), Sarah Yim, Strategy Director, Zulu Alpha Kilo (CA).
Trends In Paid Search: Navigating The Digital Landscape In 2024Search Engine Journal
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The search marketing landscape is evolving rapidly with new technologies, and professionals, like you, rely on innovative paid search strategies to meet changing demands.
Its important that youre ready to implement new strategies in 2024.
Check this out and learn the top trends in paid search advertising that are expected to gain traction, so you can drive higher ROI more efficiently in 2024.
Youll learn:
- The latest trends in AI and automation, and what this means for an evolving paid search ecosystem.
- New developments in privacy and data regulation.
- Emerging ad formats that are expected to make an impact next year.
Watch Sreekant Lanka from iQuanti and Irina Klein from OneMain Financial as they dive into the future of paid search and explore the trends, strategies, and technologies that will shape the search marketing landscape.
If youre looking to assess your paid search strategy and design an industry-aligned plan for 2024, then this webinar is for you.
5 Public speaking tips from TED - Visualized summarySpeakerHub
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From their humble beginnings in 1984, TED has grown into the worlds most powerful amplifier for speakers and thought-leaders to share their ideas. They have over 2,400 filmed talks (not including the 30,000+ TEDx videos) freely available online, and have hosted over 17,500 events around the world.
With over one billion views in a year, its no wonder that so many speakers are looking to TED for ideas on how to share their message more effectively.
The article 5 Public-Speaking Tips TED Gives Its Speakers, by Carmine Gallo for Forbes, gives speakers five practical ways to connect with their audience, and effectively share their ideas on stage.
Whether you are gearing up to get on a TED stage yourself, or just want to master the skills that so many of their speakers possess, these tips and quotes from Chris Anderson, the TED Talks Curator, will encourage you to make the most impactful impression on your audience.
See the full article and more summaries like this on SpeakerHub here: https://speakerhub.com/blog/5-presentation-tips-ted-gives-its-speakers
See the original article on Forbes here:
http://www.forbes.com/forbes/welcome/?toURL=http://www.forbes.com/sites/carminegallo/2016/05/06/5-public-speaking-tips-ted-gives-its-speakers/&refURL=&referrer=#5c07a8221d9b
ChatGPT and the Future of Work - Clark Boyd Clark Boyd
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Everyone is in agreement that ChatGPT (and other generative AI tools) will shape the future of work. Yet there is little consensus on exactly how, when, and to what extent this technology will change our world.
Businesses that extract maximum value from ChatGPT will use it as a collaborative tool for everything from brainstorming to technical maintenance.
For individuals, now is the time to pinpoint the skills the future professional will need to thrive in the AI age.
Check out this presentation to understand what ChatGPT is, how it will shape the future of work, and how you can prepare to take advantage.
The document provides career advice for getting into the tech field, including:
- Doing projects and internships in college to build a portfolio.
- Learning about different roles and technologies through industry research.
- Contributing to open source projects to build experience and network.
- Developing a personal brand through a website and social media presence.
- Networking through events, communities, and finding a mentor.
- Practicing interviews through mock interviews and whiteboarding coding questions.
Google's Just Not That Into You: Understanding Core Updates & Search IntentLily Ray
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1. Core updates from Google periodically change how its algorithms assess and rank websites and pages. This can impact rankings through shifts in user intent, site quality issues being caught up to, world events influencing queries, and overhauls to search like the E-A-T framework.
2. There are many possible user intents beyond just transactional, navigational and informational. Identifying intent shifts is important during core updates. Sites may need to optimize for new intents through different content types and sections.
3. Responding effectively to core updates requires analyzing "before and after" data to understand changes, identifying new intents or page types, and ensuring content matches appropriate intents across video, images, knowledge graphs and more.
A brief introduction to DataScience with explaining of the concepts, algorithms, machine learning, supervised and unsupervised learning, clustering, statistics, data preprocessing, real-world applications etc.
It's part of a Data Science Corner Campaign where I will be discussing the fundamentals of DataScience, AIML, Statistics etc.
Time Management & Productivity - Best PracticesVit Horky
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Here's my presentation on by proven best practices how to manage your work time effectively and how to improve your productivity. It includes practical tips and how to use tools such as Slack, Google Apps, Hubspot, Google Calendar, Gmail and others.
The six step guide to practical project managementMindGenius
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The six step guide to practical project management
If you think managing projects is too difficult, think again.
Weve stripped back project management processes to the
basics to make it quicker and easier, without sacrificing
the vital ingredients for success.
If youre looking for some real-world guidance, then The Six Step Guide to Practical Project Management will help.
Dr Andrew Makar, Tactical Project Management
Unlocking the Power of ChatGPT and AI in Testing - A Real-World Look, present...Applitools
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During this webinar, Anand Bagmar demonstrates how AI tools such as ChatGPT can be applied to various stages of the software development life cycle (SDLC) using an eCommerce application case study. Find the on-demand recording and more info at https://applitools.info/b59
Key takeaways:
Learn how to use ChatGPT to add AI power to your testing and test automation
Understand the limitations of the technology and where human expertise is crucial
Gain insight into different AI-based tools
Adopt AI-based tools to stay relevant and optimize work for developers and testers
* ChatGPT and OpenAI belong to OpenAI, L.L.C.
Unlocking the Power of ChatGPT and AI in Testing - A Real-World Look, present...Applitools
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Electrophoretic Technique Electro .pptx
1. 1
Dual Detection High-Speed Capillary
Electrophoresis (HSCE)
By: Lamees Zaro
Dr. Rasmi Abu Helu
Al-Quds University
Faculty of Graduate Studies
Department of Medical Laboratory Sciences
12/21/2024
2. 2
What is Capillary Electrophoresis?
A technique that separates molecules, such
as proteins, based on their size and charge
when subjected to an electric field.
It provides higher efficiency and throughput.
12/21/2024
3. 3
What is Serum Protein Electrophoresis (SPE)?
Serum proteins are separated based on their
charge-to-mass ratio using an electric field.
Major serum proteins albumin, 留1, 留2, 硫 , 粒 are
separated.
Abnormal distributions are often indicative of disease.
The Refractive Index (RI) method is used to detect
the proteins' migration through the capillary.
12/21/2024
4. 4
What is an Immunoassay?
Immunoassays are based on the specific binding between antigens and
antibodies.
This interaction forms an antigen-antibody complex, which can be detected
by fluorescence.
Immunoassay Detection in Capillary Electrophoresis:
The antigen-antibody complex is separated from the free antigen based on
size and charge, and fluorescence detection is used to quantify the complex.
12/21/2024
5. 5
What is Dual Detection HSCE?
HSCE combines two detection methods:
1. Refractive Index (RI) detection for serum protein electrophoresis.
2. Fluorescence detection for immunoassay measurements.
This enables the simultaneous measurement of serum proteins and antigen-
antibody interactions in one analysis.
Speed and Efficiency.
Higher Sensitivity and Specificity.
12/21/2024
6. 6
Schematic of the dual detection HSCE platform
Opallage, P. M., De Silva, M., &
Dunn, R. C. (2022)
12/21/2024
7. 7
Serum Protein Electropherograms (SPE) Measured In
Various Buffers Using HSCE Combined With BSI Detection.
Opallage, P. M., De Silva, M., &
Dunn, R. C. (2022)
12/21/2024
8. 8
Evaluates Buffer Performance For Antigen-antibody Interaction Detection
Opallage, P. M., De Silva, M., &
Dunn, R. C. (2022)
12/21/2024
9. 9
Simultaneous SPE And Fluorescence Detection For Three Different Antibody Levels.
Opallage, P. M., De Silva, M., &
Dunn, R. C. (2022)
12/21/2024
10. 10
Opallage, P. M., De Silva, M., &
Dunn, R. C. (2022)
Fluorescence Electropherograms Across Antibody Concentrations
12/21/2024
11. 11
Calibration Curve for Immunoassay Quantification
Opallage, P. M., De Silva, M., &
Dunn, R. C. (2022)
12/21/2024
12. 12
Benefits
High sensitivity and resolution.
Rapid and automated analysis.
Capability to detect both qualitative and quantitative abnormalities in serum and
urine.
12/21/2024
13. 13
Applications
Clinical diagnostics.
1. Protein disorders.
2. Immune system disorders.
3. Infectious diseases.
Research applications.
Pharmaceutical applications (therapeutic antibodies, vaccine development, drug
monitoring).
12/21/2024