Enzymes questions part 1.pdf

Sep 16, 20220 likes25 views

The document discusses enzyme inhibition in bacteria and cellular respiration. It describes the enzyme DHPS, which is involved in folic acid production in bacteria and is inhibited by the drug sulfonamide. Sulfonamide acts as a competitive inhibitor because its structure is similar to PABA, the substrate of DHPS, allowing it to bind to the active site. The document also discusses succinate dehydrogenase, an enzyme in respiration that is competitively inhibited by malonate. Increasing the concentration of succinate relative to malonate would lessen the inhibitory effect.

alevelbiology.co.uk
[Total 7 marks]
3. The enzyme DHPS is involved in the production of folic acid in bacteria.
• The substrate for DHPS is a molecule known as PABA.
• The enzyme DHPS is inhibited by the drug sulfonamide.
The figure below shows the structure of PABA and that of sulfonamide.

alevelbiology.co.uk
(i) Diagrams X, Y and Z represent these enzyme molecules and their active sites.
State the letter, X, Y or Z, that most accurately represents the enzyme DHPS.
.........................................................................................................................
[1]
(ii) Using the information in the figure above, explain why sulfonamide acts as a competitive
inhibitor of DHPS.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 4 marks]

alevelbiology.co.uk
4. An important enzyme that is used in respiration is succinate dehydrogenase. Its substrate is succinate,
which is converted into fumarate. Malonate acts as a competitive inhibitor, but does not bind
permanently to the enzyme.
(a) Describe how malonate inhibits the enzyme. You may use the space below for a diagram to help
your answer.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(b) Explain what would happen to the reaction if the concentration of succinate was increased
relative to the concentration of malonate.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 6 marks]

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Enzymes questions part 1.pdf

1. alevelbiology.co.uk 1.

2. alevelbiology.co.uk [Total 5 marks] 2.

3. alevelbiology.co.uk (a) (b) (c)

4. alevelbiology.co.uk [Total 7 marks] 3. The enzyme DHPS is involved in the production of folic acid in bacteria. • The substrate for DHPS is a molecule known as PABA. • The enzyme DHPS is inhibited by the drug sulfonamide. The figure below shows the structure of PABA and that of sulfonamide.

5. alevelbiology.co.uk (i) Diagrams X, Y and Z represent these enzyme molecules and their active sites. State the letter, X, Y or Z, that most accurately represents the enzyme DHPS. ......................................................................................................................... [1] (ii) Using the information in the figure above, explain why sulfonamide acts as a competitive inhibitor of DHPS. ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... [3] [Total 4 marks]

6. alevelbiology.co.uk 4. An important enzyme that is used in respiration is succinate dehydrogenase. Its substrate is succinate, which is converted into fumarate. Malonate acts as a competitive inhibitor, but does not bind permanently to the enzyme. (a) Describe how malonate inhibits the enzyme. You may use the space below for a diagram to help your answer. ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... [3] (b) Explain what would happen to the reaction if the concentration of succinate was increased relative to the concentration of malonate. ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... ......................................................................................................................... [3] [Total 6 marks]

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Enzymes questions part 1.pdf

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Enzymes questions part 1.pdf