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- 1. Curriculum Vitae (CV) of Dr. Md. Ashraful Haque 1. Name : MD. ASHRAFUL HAQUE 2. Name of father w ith address : Late Shaw kot Ali Vill. Kanaidanga, PO. Chandrabash, PS.Damurhuda, Dist. Chuadanga, Bangladesh. 3. a) Date of birth (according to Matric / SSC Certificate) : 1st November, 1973 b) Place of birth : Chuadanga, Bangladesh. c) Nationality : Bangladeshi (by birth). d) Permanent home address :Vill. Kanaidanga, PO. Chandrabash, PS.Damurhuda, Dist. Chuadanga, Bangladesh. e) Present address : Professor, Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh. f) Marital status : Married 4. Academic Career (fromMatriculation / SSC up to highest degree) Name of School / College / University Years attended Name of the Exams. with subjects offered* Division / Class & Place obtained Year of Obtaining Certificate or DegreeFrom To Karpashdanga High School, Chuadanga. 1983 1988 SSC (Science) First Division 1988 Kushtia Government College, Kushtia. 1988 1990 HSC (Science) First Division 1990 Bangladesh Agricultural University, Mymensingh. 1991 1995 B.Sc.Ag (Hons) First Class & First Position in order of merit 1995 (Exam. held in 1999) Bangladesh Agricultural University, Mymensingh. 2000 2001 MS in Genetics and Plant Breeding First Class w ith Distinction (Grade A+ ) December, 2001 Tokyo University of Agriculture and Technology, Japan. 2006 2009 Doctor of Philosophy in Biochemistry and Biotechnology Aw arded September , 2009 Ohio State University, USA. 2011 2011 Post doctoralresearch on Molecular Genetics of plant pathogens Aw arded (Special recognition aw ard by the President of the Ohio State University, USA) December, 2011 * Please see the Appendix-Ifor name of the examinations w ith subjects offered 5. Academic awards * National Science and Technology aw ard offered by the Ministry of Science and Technology, Bangladesh during 2013-2014 * Special recognition aw ard-2011 through post doctoralresearch by the Ohio State University, USA. * Special recognition aw ard-2011 by IDB as an excellent researcher. * IDB Merit Scholarship-2011 for a post doctoral researchin the Ohio State University, USA. * Monbukagakushu Scholarship for Special3 years DoctoralProgram offered by the Ministry of Education, Culture, Sports Science and Technology- Japan from October, 2006 to September, 2009. * President Gold Medal aw ard offered by the Chancellor of Bangladesh AgriculturalUniversity during February, 2003. * Vice-Chancellor aw ard offered by the Vice-Chancellor of Bangladesh AgriculturalUniversity during February, 2003. * National Science and Technology aw ard offered by the Ministry of Science and Technology, Bangladesh during 2000-2001. * Shitabjan Memorial Trust aw ard offered by the Vice-Chancellor of Bangladesh Agricultural University during 2000.
- 2. 6. Membership * Life member of Plant Breeding and Genetics Society of Bangladesh. * Life member of Bangladesh Association of Biotechnology. * Life member of IDB Graduate Association in Bangladesh. 7. Training Program * Successfully completed a course on General English from British Council, Bangladesh. *Successfully completed a Postgraduate Certificate course on Seed Technology. *A training program offered by Graduate Training Institute on Scientific Report Writing * A training program offered by Graduate Training Institute on Teaching Methods and Techniques *A training program offered by Graduate Training Institute on Statistical Methods of Analysis * A training program offered by Graduate Training Institute on Project Cycle Management * A training program offered by Graduate Training Institute on Web page Design and Hosting * A training program offered by Graduate Training Institute on ISWS-510 Facilitating Learning * A field trip offered by Bangladesh AgriculturalUniversity on Agriculturalextension w orkin field level * A w orkshop on Women for Women offered by Bangladesh AgriculturalUniversity. 8. Research Interest 1. Functional analysis and characterization of plant genes related to defense and regulation mechanism against bi otic and environmental stresses. 2. Expression pattern of biotic stress related transcription factors/genesand signaltransduction. 3. Functional and promoter activities of pathogenesis related genes. 4. In vitro study of some major crops for their screening based on yield contributing characters 5. Development and establishment of transgenic plants w ith suitable genes. 9. Expertise in Advanced Laboratory Techniques Collection of data, statisticalanalysis of data, graphical representation, design of experiment, RNA, DNA and Protein extraction & purification, gelelectrophoresis, Polymerase Chain Reaction (PCR), Reverse Transcriptase Polymerase Chain Reaction (RT-PCR), Cloning, GFP and RFP fusion, Yeast one and tw o hybrid system, Sequencing and gene silencing, Agrobacterium-mediated transformation in plants, Multiple sequence alignment by using BLAST , Designing Primer and Vector, SDS-Page, Western blotting. Microscopy, CytologicalOperations, Embryo and anther culture of plants and hybridization of crop plants etc. 10. Names and addresses of persons to whom a reference maybe made: i) Lucy R. Stewart Research Molecular Biologist USDA-ARS and Adjunct Assistant Professor Departmet of Plant Pathology/OARDC,Selby Hall,Room No. 020,Wooster,Ohio-44691 The Ohio State University USA E-mail: lucy.stewart@ars.usda.gov Phone:01-330-260-3834 Field of Specialization:Molecular Breeding and Genetics ii) Hiroshi NYUNOYA Professor of Gene Research Center Tokyo University of Agriculture and Technology,Japan E-mail: nuynoya@cc.tuat.ac.jp Phone: 81-42-367-5563 Fax: 81-42-367-5840
- 3. 11. List of Publications of Dr. Md. Ashraful Haque 1. M. Chakraborty, T. Quddus, S. Rahman, M. A. K. Azad, M. R. Hossain, A. Hoque, M. K. Saha & M. A. Haque. (2015). Molecular characterization of selected mutant lines of Onion (Allium cepa L.) against Purple Leaf Blotch disease using SSR markers. American Jour. Expt. Agric. 8(4):261-267. 2. Sayed Raihanul Haider†, Mohammad Rashed Hossain†, Shanjida Rahman, Shirin Sultana, Tamanna Quddus, Moutoshi Chakraborti, Aunamika Hoque, Md Hasan Shahriar and Md AshrafulHaque*. (2015). In Vitro plantlet regeneration of four localgarlic (Allium sativum) accessions of Bangladesh. British Biotech. Jour. 8(3): 1-12). 3. Shanjida Rahman, Md. Mukul Mia, Tamanna Quddus, LutfulHassan and Md. Ashraful Haque. (2015) Assessing Genetic diversity of Maize (Zea mays L.) genotypes for agronomic traits. Res. Agric. Livest. Fish. 2(1): 1-8. 4. S. Rahman, U. Mondal, M. M. Mia, L. Hassan and M.A. Haque (2014). Wsm gene mediated resistance regulation in corn (Zea mays L.) viruses. 9th Bienn. Con. PBGSB, Dhaka. P. 75-76. 5. Md. AshrafulHaque and Mou, D. R. (2015). Screening of Tomato genotypes against Tm 1 gene for Tomato mosaic virus resistance. 2nd ICBHA-GNOBB, Univ. Dhaka. P.108-110. 6. S. Rahman, U. Mondal, M. M. Mia, L. Hassan and M.A. Haque (2014). Wsm gene mediated resistance regulation in corn (Zea mays L.) viruses. 9th Bienn. Con. PBGSB, Dhaka. P. 75-76. 7. M. Chakraborty, M. A. K. Azad, S. N. Begum, M. M. Islam, A. Hoque, M. K. Saha and M. A. Haque . (2014). Molecular characterization of selected mutant lines of Onion against Purple Blotch using SSR markers. 9th Bienn. Con. PBGSB, Dhaka. P. 78. 8. M. A. Haque, Haider, S. R., A. Hoque & Chakraborty, M. (2013). Screening of Local Garlic Strains and production of Chromosomal Variants under in vitro condition. BAU. Res. Prog. (2013) p.32. 9. Lucy R. Stew art • Md. AshrafulHaque , Mark W. Jones • Margaret G. Redinbaugh. (2013). Response of maize (Zea mays L.) lines carrying Wsm1, Wsm2 and Wsm3 to the potyviruses Johnsongrassmosaic virus and Sorghum mosaic virus. Mol. Breed. 31:289–297 DOI 10.1007/s11032-012-9789-5. 10. Haque, M. A., Shanjida, R., Mou, D. R., Moutoshi, C. & Aunamika, H. (2013). Low- cost Eco-friendly Virus free Maize cultivation for poverty alleviation and food security in Bangladesh through Molecular marker aided Breeding .BAU Res. Prog. (2013) p.39.(A very preliminary report in Bangladesh). 11. Lucy R. Stewart • Md. Ashraful Haque, Mark W. Jones • Margaret G. Redinbaugh. (2012). Response of maize (Zea mays L.) lines carrying Wsm1, Wsm2 and Wsm3 to the potyviruses Johnsongrass mosaic virus and Sorghum mosaic virus. American Phytopath. Soc. (2012). 12. Md. Ashraful Haque, Mark W. Jones, M. G., Redinbaugh and Stew art L.R. (2011). Screening of corn (Zea mays L.) genotypes w ith introgressed Wsm loci for resistance to the potyviruses Johnsongrass mosaic virus (JGMV) and Sorghum mosaic virus (SrMV). Sixth Annu. Res. Exp.-2011 by the Ohio State University, USA. 13. Mayumi Takano, Md. Ashraful Haque, Shota Odaira1, Keiko Nakata, Nobumitsu Sasaki and Hiroshi Nyunoya. (2013). Overexpression of a tobacco Dof transcription factorBBF1 stimulates the transcription of the tobacco mosaic virus resistance gene N and defense-related responses including ROS production.. Plant. Biotech. 30, 37-46 DOI: 10.5511/plantbiotechnology.12.1207a 14. M. A. Haque, M. A. Mahmud, M. M. Islam and S. N. Begum. (2012). Identification of introgressed rice lines of Binadhan-7×FL-378 under salt stress through SSR markers. J. Bangladesh Agril. Univ. 10(1): 49–54. 15.Mayumi, T., Odaira, S., Md. A. Haque, Sasaki, N. and Nyunoya, H. (2011). Overexpression of Dof transcription factor accelerates hypersensitive response to the elicitor of Tobacco Mosaic Virus through the upregulation of the tobacco resistance gene N. 8th Solanaceae and 2nd Cucurbitaceae joint conference, Kobe,Japan. 16. Sasaki, N., Haque, M. A., Mayumi, T., Odaira, S. and Nyunoya, H. (2011).Transcriptional transactivation of the tobacco resistance gene N for Tobacco Mosaic Virus through its own product N protein. World Congress of Microbes- 2011, Beijing, China. 17. Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Seisuuke Mimori, Jun-Shan Gao and Hiroshi Nyunoya. (2009). Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N. Physiol Mol Plant Pathol 73, 101-108. 18. Haque, M. A.,Yasmin M. R., Hossain, M. S. Mia, M. S. and M. S. Kabir. (2010). Effect of growth hormones on micropropagation of Garlic. Bangladesh J. crop. Sci. 21 (1): 19-23.
- 4. 19. Sasaki, N., Haque, M. A., Matsumaru, M., Odara, S., Nyunoya, H. (2010).Involvement of tobacco transcription factor Dof proteins in the promoter activation of the resistance gene N against Tobacco mosaic virus. 21st International Conference on Arabidopsis research held in Yokohama, Japan,p-65. 20. Haque, M. A., Yasmin, M. R., Kabir, A. T. M. A. , Firoz, S. M. S.and Jamal, M.R. (2010). In vitro regeneration and transformation of Chickpea (Cicer arietinum L.) via grafting. Bangladesh J. crop. Sci. 21 (1&2):151-158. 21. M. M. Khatun, Hossain, M. S., Haque, M. A. and M. Khalekuzzaman. (2010). In Vitro propagation of Citrullus Lanatus Thumb. From nodal explants culture. J. Bangladesh Agril. Univ. 8(2):203-206. 22. Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Jun-Shan Gao and Hiroshi Nyunoya. (2009). Elicitor responsive 20-bp element of the Tobacco mosaic virus resistance gene N. XIV international congress on Mol. Plant Microbe Inter. (MPMI), p- 72. 23. Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Jun-Shan Gao and Hiroshi Nyunoya. (2009). Study on the regulation of the elicitor-responsive expression of Tobacco mosaic virus resistance. The international congress on Molecular Biology held in Meiji, Japan. 24. Md. Ashraful Haque, Nobumitsu Sasaki, Matsumaru Masamichi and Hiroshi Nyunoya. (2009). Functional involvement of tobacco Dof protein in the resistance to Tobacco mosaic virus.The international congress on Biochemistry and Molecular Biology held in Yokohama, Japan. 25. Haque, M. A., Yasmin M. R., Rajesh, S and Hassan, L. (2009). In vitro screeing of rapeseed and mustard genotype for salt tolerance. Bangladesh J. Enviorn. Sci. 17:74-79. 26. Haque, M. A., Yasmin M. R., Dey, R and Hassan, L. (2009). In vitro regeneration of rice. Bangladesh J. Enviorn. Sci. 17: 92-97. 27. Yasmin M. R. and Haque, M. A. (2009). Micropropagation through stem disc culture and its growth parameters affecting bulblet formation of garlic. Bangladesh J. Enviorn. Sci. 16: 243-248. 28. Md. Ashraful Haque, Nobumitsu Sasaki and Hiroshi Nyunoya. (2008). Identification of a cis regulatory element of the Tobacco mosaic virus resistance gene N. The international congress on Biochemistry and Molecular Biology held in Kobe, Japan, p- 501. 29. Md. Ashraful Haque. (2007). Analyses of virus resistance N gene promoter of Nicotiana tabacum. A congress arranged by the United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology held in Chiba, Japan. 30. Haque, M. A. and M. R. Yasmin. (2006). Determination of total Sulphur in local cultivars of Garlic. Bangladesh J. Crop. Sci.17 (1) 251-255. 31. Haque, M. A. and M. R. Yasmin. (2006).Sulfur utilization and Genotype- Environment interaction in Garlic. Bangladesh J. Enviorn. Sci. 12 (1): 150-154. 32. Hossain, M. S., M. Khalekuzzaman, O. I. Joarder, Haque, M.A. and M. M. Rahman. (2006). Multivariate Analysis in Rice Genotypes. Bangladesh J. Crop. Sci.17 (1) 7-12. 33. Yasmin, R., Haque, M. A. and Hassan, L. (2005).Comprehensive in vitro regeneration and screening of virus free Garlic cultivars. J. Bangladesh. Soc. Agril. Sci.Technol. 2 (3 and 4):125-128. 34. Haque, M.A. and M.S. Alam. (2004). In vitro shoot regeneration and Plantlet formation in Garlic. J. Bangladesh. Soc. Agril. Sci. Technol. 1 and 2(1): 54-60. 35. Haque, M.A. (2004). Chromosome Identification in Papaya (Carica papaya). J. Bangladesh Agril. Univ. 2(1):15- 20. 36. Haque, M.A. (2003). Production of chromosomal variant in Garlic using colchicine under in vitro condition. Progres. Agric. 14(1 and 2): 57-60. 37. Haque, M. A. (2003). Efficacy of sucrose on direct regeneration and rapid bulblet formation in Garlic (Allium sativum). Bangladesh J. Enviorn. Sci. 9(2): 205-211. 38. Haque, M. A., Ahmad, Q.N. and Alam, M. S. (2002). Somatic embryogenesis and formation of microbulbil under in vitro regeneration of Garlic (Allium sativum). Bangladesh J. Enviorn. Sci. 8: 87-90. 39. Haque, M. A., Nath, U.K, Ahmad, Q.N and Alam, M.S. (2003). Effect of 2, 4-D and BAP on in vitro regeneration of Garlic (Allium sativum). Online J. Biol. Sci. 2(12): 771-774.
- 5. 40. Hossain, M.A., Karim, M.R., Begum, S., Hossain, M.A and Haque, M. A. (2002). Effect of Cephalexin on sex expression, fruit development and yield of Cucumber (Cucumis sativusL.). Online J. Biol. Sci. 2 (10): 656-658. 41. Datta, S., Rahman. M.M., Haque, M. A., Islam, M.M. and Mollah, M.F.A.(2002). Chromosomal studies on Ompok pabda (Hamilton). Bangladesh J. Enviorn. Sci. 8:179-182. 42. Takano,M., Odaira,S., Haque, M. A., Nakata,K., Sasaki, N., and Nyunoya, H. (2013). Overexpression of the Dof protein in tobacco leads to transcriptional activation of the resistance gene N, suggesting potential roles in the regulation of hypersensitive response to TMV. ICAR-2013, Univ. West. Aus. held in 24-28 June, 2013. Appendix-I:Name of the Examinations withSubjects Offered Name of the Examinations Subjects offered Secondary School Certificate (SSC) Bengali, English, General Mathematics, Geography, General Science (Ist & 2nd paper), Elective Mathematics and Religious Education. Higher Secondary Certificate (HSC) Bengali, English, Physics, Chemistry, Mathematics and Biology. Bachelor of Science in Agriculture (BSc Ag) Agronomy, Soil Science, Horticulture, Crop Botany, Entomology, Plant Pathology, Genetics & Plant Breeding, Agricultural Extension Education, Agricultural Chemistry, Biochemistry, Chemistry, Agricultural Economics, Animal Husbandry, Farm Mechanics, Agricultural Statistics, and Rural Sociology. Master of Science (MS) in Genetics and Plant Breeding Cytology and Embryology, Biometrical and Population Genetics, Crop Evolution and Plant Genetic Resources, Cytogenetics, Distant Hybridization and Chromosome Manipulation, Biotechnology and Genetic Engineering, Principles of Plant Breeding, Molecular and Developmental Genetics, Molecular Breeding, Molecular Genetics, Experimentation and Data Analysis. Thesis Title: Individual chromosome identification through karyotype analysis and production of tissue culture regenerants in garlic. Doctor of Philosophy in the division of Applied Biological Science. Post doctoral research on Molecular Genetics of plant virus resistance in Maize Chromosomal evolution and gene expression in higher plant, Molecular aspects in plant- pathogen interaction, Structure and function of extracellular matrix, Bioactive material chemistry, Intermolecular interaction and super molecular chemistry, Genome mapping and breeding in crop plants, Versatile function of RNA, Carbon reduction w ith forest resources and other courses offered by the United Graduate School of Agricultural Science at Tokyo University of Agriculture and Technology in collaboration with other member Universities. Thesis Title: Study on the regulation of the elicitor-responsive expression of Tobacco mosaic virus resistance gene N Post doctoral research mainlyfocused on Corn virus resistance mechanism