Lab 12 lv
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This lab used an ELISA test to simulate the sharing of bodily fluids and determine which students were exposed to and transmitted a contagious disease. The ELISA test relies on antibodies to detect antigens produced during an immune response to exposure. The results showed students 1, 2, 8, and 10 were infected, while students 1 and 10 transmitted the disease. ELISA is commonly used in real world applications such as pregnancy tests, disease detection, and testing for food safety and GMOs.
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Lab 12 e
Lab 12 evalrivera
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This lab used an ELISA test to simulate the sharing of a contagious disease between students and determine who was infected and who transmitted the disease. The ELISA test relies on antibodies binding to antigens produced during an immune response to detect exposure to a disease in liquid samples. The results of the lab showed that students 1, 2, 8, and 10 were infected, while students 1 and 10 transmitted the disease to others.Seminario de biomol
Seminario de biomolCristianRamirez283
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Antimicrobial resistant Escherichia coli isolates detected in raw milk of
livestock in pastoral areas of northern KenyaValenzuela Nilda abstract DDB
Valenzuela Nilda abstract DDBNilda Valenzuela
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1) Cuterebra is a parasite of rodents and lagomorphs that can infect cats, causing disease by migrating through tissues including eyes, respiratory tract, and brain.
2) An ELISA was used to test 247 cat sera samples from rural New England for antibodies to Cuterebra, using crude larval antigens.
3) 16 samples had over a 75% probability of infection, and 32 samples had over a 50% probability, indicating exposure to Cuterebra is present in the cat population of rural New England.Tutorial 1
Tutorial 1UiTM Jasin
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This document contains questions about various microbiology concepts including:
- Koch's postulates
- The theory of spontaneous generation
- The differences between simple and compound microscopes
- Who initiated agar and pure culture techniques
- Key differences between prokaryotes and archaea
- Proper naming conventions for E. coli and S. aureus
- Uses of special, gram, and negative staining
- Bacteria commonly found on eggshells
- The virus that preys on bacteria
- What plaque is
- How bacteria can benefit us
- Who pioneered hand washing in hospitals
- Pasteur's swan neck flask experiment
- Why bacterial spores pose problems for sterilization
-David Meyer, PhD Welcome Remarks & Introduction to LA BioMed
David Meyer, PhD Welcome Remarks & Introduction to LA BioMedUCLA CTSI
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David Meyer, PhD Welcome Remarks & Introduction to LA BioMed
President/CEO, Los Angeles Biomedical Research Institute, Harbor-UCLA Medical Center
Student Researcher studies development of bluebirds through their 'droppings'
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Colleen Elzinga, a chemistry-biology major at Ripon College, studied the development of eastern bluebirds through analyzing hormones in their droppings over the summer. She found that examining hormone levels in droppings was more effective than blood because droppings are easier to obtain and provide a longer view of hormone levels over time. By grinding, drying, and purifying droppings samples before testing, Elzinga was able to determine testosterone levels and learn more about the growth and age of individual bluebirds. Her work provides techniques for other researchers to similarly study hormone levels and growth using excrement samples.FIRST PLASTIC CELL WITH WORKING ORGANELLE AND MODIFIED PROTEINS AS VACCINES A...
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Scientists developed artificial cells using polymers that were able to perform the same chemical reactions as natural cells. They created organelles like membranes, ribosomes, and mitochondria in the artificial cells. Using fluorescence, they confirmed that the planned cascade of intracellular reactions took place as intended. Separately, researchers identified the Pru p 3 protein as the cause of peach allergies. They developed two modified versions, Pru p 3.02 and Pru p 3.03, for use in immunotherapy to treat patients with peach allergies. While more animal trials are still needed, this research establishes a new approach for immunotherapy and has potential applications for treating other food allergies and autoimmune diseases.Valeria lab 2 lv
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The objectives of this lab were to learn how to use a burette and spectrophotometer to determine the absorbance and molarities of solutions. Students used a potassium permanganate mother solution to create serial dilutions through a burette. They measured aliquots of the dilutions and analyzed them using a spectrophotometer to collect absorbance data, which was then graphed in a linear regression by the professor to show the relationship between concentration and absorbance. Students also calculated molarities of the original solutions.Rivera valeria same
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This study aimed to confirm previous findings linking canine chromosomes 1 and 19 to idiopathic epilepsy in Australian Shepherd dogs. PCR-RFLP tests of two SNPs were performed on 88 additional Australian Shepherds and genotypes were compared between affected and unaffected dogs via Chi-square analysis. Sequencing of the DOK6 gene did not reveal differences between one affected and unaffected dog. The results confirmed different genotype frequencies between normal and affected dogs for the SNPs, supporting the hypothesis that epilepsy-causing genes are located near the tested regions on chromosomes 1 and 19.Vateria lab 8lv
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The laboratory aimed to separate proteins expressed in E. coli after transformation using SDS-PAGE. Samples from bacteria colonies containing the pGLO plasmid were prepared with and without heat treatment. The samples were run on a polyacrylamide gel, which was then divided and stained with Coomassie blue or left unstained. The unstained gel showed fluorescence at 37kD in lane 8, while lanes 9-10 showed complete protein denaturation. The stained gel demonstrated a variety of E. coli proteins from 10-160kD and showed GFP denaturation at 27kD and partial denaturation at 37kD in lanes 3 and 5.University of minnesota blog photos
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The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.University of minnesota blog photos
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The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.Aussies v
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This document summarizes research being done to identify genetic factors that cause epilepsy in Australian Shepherd dogs. The researchers are genotyping additional dogs to confirm associations between epilepsy and SNPs on chromosomes 1 and 19 found in previous work. They plan to sequence the DOK6 gene near one of the SNPs on chromosome 1 to look for mutations correlated with epilepsy. Current work includes genotyping 80 additional dogs and sequencing exons of the DOK6 gene, though one exon is still being analyzed. The goal is to identify the specific genetic cause of epilepsy in this breed to improve treatment and prevent transmission.The epilepsy paper 3
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The study confirms associations between epilepsy in Australian Shepherd dogs and two locations on chromosomes 1 (CFA1) and 19 (CFA19) found in previous research. PCR-RFLP tests on 88 additional dogs showed different genotype frequencies between normal and affected dogs, supporting genes in these locations that may cause epilepsy. Sequencing of the DOK6 gene near associated SNPs on CFA1 found no differences between one affected and unaffected dog. The results support the hypothesis that mutations in these chromosomal regions contribute to epilepsy in Australian Shepherds.Proposal final
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This study aims to clone and analyze GAPDH genes from various plant species to compare amino acid sequences related to catalytic function. Previous work successfully cloned GAPDH from Oxalis corniculata and Plectranthus amboinicus but not Myrtaceae psidium. The current study will continue work on the cloned samples through midipreps, restriction enzyme digestion and sequencing. Sequence data will undergo bioinformatics analysis to compare conserved amino acids of the GAPDH protein between plant species. Results could provide new genetic information published in GenBank and further the understanding of energy production pathways in plants.Valeria laboratory 7lv
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This laboratory experiment transformed E. coli bacteria with a pGLO plasmid containing the GFP gene from jellyfish using electroporation and heat shock. Students used the GFP gene as a visual marker to observe if transformation was successful by looking for fluorescence in bacterial colonies grown on agar plates containing arabinose, which regulates GFP protein expression. Only bacterial colonies grown on plates with ampicillin and arabinose exhibited fluorescence, confirming successful transformation and expression of the GFP gene.Valeria lab 6lv
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This document summarizes a lab assignment on bioinformatics. Students were asked to practice using bioinformatics tools like BLAST and databases from NCBI to analyze DNA sequences. For the assignment, students were given a scenario where they had to use these tools to identify which staff members at a research project were illegally using DNA from an endangered primate species. Students performed BLAST searches comparing DNA samples from staff members to a sequence from the endangered primate provided by another company, identifying that two staff members were implicated.Lysosomal storage disease 2
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Synapses are specialized structures that transmit signals between neurons or from neurons to other cells. They convert electrical signals into chemical signals to allow communication between cells by releasing neurotransmitters in response to action potentials. Malfunctions in synaptic transmission can cause disorders like epilepsy, neuropathic pain, anxiety, depression, learning deficits, and dementia. The study focuses on how a calcium channel subunit called a25 that is targeted by anti-epileptic and pain drugs regulates synaptic transmission and structure.Elisa immuno
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The student performed an ELISA technique in their laboratory class to simulate the spread of an infectious disease. The ELISA uses antibodies to detect disease agents in body fluids. In this simulation, the primary antibody was rabbit which recognized the chicken antigen, while the secondary antibody was goat which recognized the primary rabbit antibody. By testing sample #10 which came up positive, they determined that sample belonged to the person exposed to the contagious disease in the 5 minute incubation period.Elisa
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An ELISA test was performed on hypothetical samples from a group where some individuals exchanged bodily fluids, with one person carrying HIV. The ELISA allowed them to determine which samples were infected with HIV and identify the original couple where transmission occurred, in order to understand the spread of the infection within the group.Detection of Pathogenic Strains of Entamoeba Histolytica in Children Using EL...
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Immunodiagnostics uses antigen-antibody reactions to detect substances like hormones, microbes, and drugs. Precipitation reactions are a type of immunodiagnostic assay that detects antibody levels in serum. They work by allowing antibodies and antigens to diffuse toward each other in semi-solid media and form a visible precipitate line. Common precipitation methods include Ouchterlony test and Mancini test, which are based on antibodies and antigens forming insoluble complexes.APPLICATIONS OF ELISA .pptx (Introduction,principle and applications)
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Gives information regarding general principle of ELISA and various applications of it 
Elisa
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ELISA is a biochemical technique used in immunology to detect the presence of an antibody or antigen in a sample. It involves coating microtiter plate wells with an antigen or antibody and using conjugated enzymes and substrates to produce a colored product to indicate a positive result. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA which are used to test for various antigens or antibodies. ELISA has many applications such as measuring serum antibody concentrations, detecting food allergens or diseases, and identifying past exposure to diseases.Elisa
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ELISA (enzyme-linked immunosorbent assay) is a popular biochemistry assay used to detect the presence of a substance like an antigen in a liquid sample. It involves using at least one antibody with specificity for a particular antigen. In ELISA, a liquid sample is added to a solid phase and multiple liquid reagents are sequentially added and incubated followed by an optical change like color development that is measured to quantify the analyte. ELISA has been used as a diagnostic tool in medicine and other industries since the 1960s. It has four main types - indirect, direct, competitive, and multiple/portable - and applications like detecting antibodies, allergens, drugs, and diseases.16 4 2010_0362_0364
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ELISA (Enzyme-Linked Immunosorbent Assay) is a laboratory technique used to detect and quantify specific proteins, antibodies, antigens, or other molecules in a sample. It is based on the principle of using antibodies to capture and detect the presence of a target substance.The technique is named sandwich because the analyte of interest is sandwiched between two layers of antibodies the capture antibody and the detection antibody. This layering enhances its precision.
Sandwich ELISA is indispensable in identifying low-abundance proteins, making it critical in fields like immunology, microbiology, and biochemistry.
BIOTEM: Covid-19 and Serological Tests 
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Serological tests detect antibodies in blood samples and can be used to identify individuals exposed to infectious diseases like COVID-19. Enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFIAs) are commonly used serological test methods. ELISAs are often used in laboratories to detect and quantify antibodies, while LFIAs can provide rapid, point-of-care testing using strips similar to pregnancy tests. Both tests aim to specifically detect antibodies produced against target antigens, helping clinicians understand infection histories.More Related Content
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This study aimed to confirm previous findings linking canine chromosomes 1 and 19 to idiopathic epilepsy in Australian Shepherd dogs. PCR-RFLP tests of two SNPs were performed on 88 additional Australian Shepherds and genotypes were compared between affected and unaffected dogs via Chi-square analysis. Sequencing of the DOK6 gene did not reveal differences between one affected and unaffected dog. The results confirmed different genotype frequencies between normal and affected dogs for the SNPs, supporting the hypothesis that epilepsy-causing genes are located near the tested regions on chromosomes 1 and 19.Vateria lab 8lv
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The laboratory aimed to separate proteins expressed in E. coli after transformation using SDS-PAGE. Samples from bacteria colonies containing the pGLO plasmid were prepared with and without heat treatment. The samples were run on a polyacrylamide gel, which was then divided and stained with Coomassie blue or left unstained. The unstained gel showed fluorescence at 37kD in lane 8, while lanes 9-10 showed complete protein denaturation. The stained gel demonstrated a variety of E. coli proteins from 10-160kD and showed GFP denaturation at 27kD and partial denaturation at 37kD in lanes 3 and 5.University of minnesota blog photos
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The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.University of minnesota blog photos
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The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.Aussies v
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This document summarizes research being done to identify genetic factors that cause epilepsy in Australian Shepherd dogs. The researchers are genotyping additional dogs to confirm associations between epilepsy and SNPs on chromosomes 1 and 19 found in previous work. They plan to sequence the DOK6 gene near one of the SNPs on chromosome 1 to look for mutations correlated with epilepsy. Current work includes genotyping 80 additional dogs and sequencing exons of the DOK6 gene, though one exon is still being analyzed. The goal is to identify the specific genetic cause of epilepsy in this breed to improve treatment and prevent transmission.The epilepsy paper 3
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The study confirms associations between epilepsy in Australian Shepherd dogs and two locations on chromosomes 1 (CFA1) and 19 (CFA19) found in previous research. PCR-RFLP tests on 88 additional dogs showed different genotype frequencies between normal and affected dogs, supporting genes in these locations that may cause epilepsy. Sequencing of the DOK6 gene near associated SNPs on CFA1 found no differences between one affected and unaffected dog. The results support the hypothesis that mutations in these chromosomal regions contribute to epilepsy in Australian Shepherds.Proposal final
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This study aims to clone and analyze GAPDH genes from various plant species to compare amino acid sequences related to catalytic function. Previous work successfully cloned GAPDH from Oxalis corniculata and Plectranthus amboinicus but not Myrtaceae psidium. The current study will continue work on the cloned samples through midipreps, restriction enzyme digestion and sequencing. Sequence data will undergo bioinformatics analysis to compare conserved amino acids of the GAPDH protein between plant species. Results could provide new genetic information published in GenBank and further the understanding of energy production pathways in plants.Valeria laboratory 7lv
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Synapses are specialized structures that transmit signals between neurons or from neurons to other cells. They convert electrical signals into chemical signals to allow communication between cells by releasing neurotransmitters in response to action potentials. Malfunctions in synaptic transmission can cause disorders like epilepsy, neuropathic pain, anxiety, depression, learning deficits, and dementia. The study focuses on how a calcium channel subunit called a25 that is targeted by anti-epileptic and pain drugs regulates synaptic transmission and structure.Similar to Lab 12 lv (20)
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The student performed an ELISA technique in their laboratory class to simulate the spread of an infectious disease. The ELISA uses antibodies to detect disease agents in body fluids. In this simulation, the primary antibody was rabbit which recognized the chicken antigen, while the secondary antibody was goat which recognized the primary rabbit antibody. By testing sample #10 which came up positive, they determined that sample belonged to the person exposed to the contagious disease in the 5 minute incubation period.Elisa
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Immunodiagnostics uses antigen-antibody reactions to detect substances like hormones, microbes, and drugs. Precipitation reactions are a type of immunodiagnostic assay that detects antibody levels in serum. They work by allowing antibodies and antigens to diffuse toward each other in semi-solid media and form a visible precipitate line. Common precipitation methods include Ouchterlony test and Mancini test, which are based on antibodies and antigens forming insoluble complexes.APPLICATIONS OF ELISA .pptx (Introduction,principle and applications)
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ELISA is a biochemical technique used in immunology to detect the presence of an antibody or antigen in a sample. It involves coating microtiter plate wells with an antigen or antibody and using conjugated enzymes and substrates to produce a colored product to indicate a positive result. There are different types of ELISA including direct, indirect, sandwich, and competitive ELISA which are used to test for various antigens or antibodies. ELISA has many applications such as measuring serum antibody concentrations, detecting food allergens or diseases, and identifying past exposure to diseases.Elisa
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ELISA (enzyme-linked immunosorbent assay) is a popular biochemistry assay used to detect the presence of a substance like an antigen in a liquid sample. It involves using at least one antibody with specificity for a particular antigen. In ELISA, a liquid sample is added to a solid phase and multiple liquid reagents are sequentially added and incubated followed by an optical change like color development that is measured to quantify the analyte. ELISA has been used as a diagnostic tool in medicine and other industries since the 1960s. It has four main types - indirect, direct, competitive, and multiple/portable - and applications like detecting antibodies, allergens, drugs, and diseases.16 4 2010_0362_0364
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This study evaluated the sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) test for detecting Giardia lamblia antigen in stool samples, compared to microscopic examination. Of 84 stool samples from children in Duhok, Iraq, 42 were positive and 42 negative for G. lamblia by microscopy. The ELISA test detected all 42 microscopy-positive samples, plus an additional 13 microscopy-negative samples, resulting in a sensitivity of 76.4% and specificity of 100% compared to microscopy. The study recommends using ELISA for epidemiological surveys in Iraq and to confirm diagnoses when microscopy is negative but clinical symptoms are present.What is Sandwich ELISA - CytoScientific
What is Sandwich ELISA - CytoScientificCyto Scientific
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ELISA (Enzyme-Linked Immunosorbent Assay) is a laboratory technique used to detect and quantify specific proteins, antibodies, antigens, or other molecules in a sample. It is based on the principle of using antibodies to capture and detect the presence of a target substance.The technique is named sandwich because the analyte of interest is sandwiched between two layers of antibodies the capture antibody and the detection antibody. This layering enhances its precision.
Sandwich ELISA is indispensable in identifying low-abundance proteins, making it critical in fields like immunology, microbiology, and biochemistry.
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油
Serological tests detect antibodies in blood samples and can be used to identify individuals exposed to infectious diseases like COVID-19. Enzyme-linked immunosorbent assays (ELISAs) and lateral flow immunoassays (LFIAs) are commonly used serological test methods. ELISAs are often used in laboratories to detect and quantify antibodies, while LFIAs can provide rapid, point-of-care testing using strips similar to pregnancy tests. Both tests aim to specifically detect antibodies produced against target antigens, helping clinicians understand infection histories.Elissa test 
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The document discusses ELISA (enzyme-linked immunosorbent assay), a test that detects antibodies in blood and can determine if a person has antibodies related to certain infections. ELISA works by using antigens and antibodies; antigens are proteins on pathogens that induce antibody production, and antibodies are proteins produced in response to antigens. ELISA relies on the principle that antibodies will bind to target antigens, allowing detection of antigen-antibody concentrations. It can detect antigen or antibody presence and concentrations, and is used for applications like screening blood donations and measuring hormone levels.Molecular Biology Techniques (ELISA)
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The document summarizes two studies that used ELISA techniques to evaluate immunity against viruses and detect parasites. The first study developed a competitive ELISA to evaluate immunity to the Varicella-Zoster virus by measuring neutralizing antibodies against viral proteins. The second study used a monoclonal antibody-based sandwich ELISA and immunochromatographic assay to detect the cathepsin L1 protease antigen in detecting Fasciola gigantica infection. Both investigations validated ELISA as an accurate method to establish an individual's immune status and identify the presence of antigens.
Applications of immunological functions(Sp13-bty-001) CIIT Abbottabad 
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Applications of Immunological functions
Introduction
Immunological function is define as biological effects of immune system on antigens during immune responds
Effects may be
1.Pathological effects (By keeping normal homeostasis)
2.Physiological effects (Resulting in diseases)
Main functions of immunity
1.Defense
2.Homeostasis
3.Surveillance
Physiological and pathological representation of immune response
Function Physiological
(advantageous) Pathological
(harmful)
1.Immune defense Resist to pathogen Immunologic deficiency disease
2.Immune homeostasis scavenge damaged Autoalergic disease
3.Immune surveillance Scavenge cells with misreplication Cell cancerization persistent infection
Applications of Immunological functions
1.Treatment (Vaccines)
Immunity to a disease is achieved through the presence of antibodies油to that disease in a person system. Antibodies are proteins produced by the body to neutralize or destroy toxins or disease-carrying organisms. Antibodies are disease specific. For example, measles antibody will protect a person who is exposed to measles disease, but will have no effect if he or she is exposed to mumps.
There are two types of immunity: active and passive.
a)Active Immunity
It油results when exposure to a disease organism triggers the immune system to produce antibodies to that disease. Exposure to the disease organism can occur through infection with the actual disease (natural immunity), or introduction of a killed or weakened form of the disease organism through vaccination (vaccine-induced immunity). If an immune person comes into contact with that disease in the future, their immune system will recognize it and immediately produce the antibodies needed to fight it. Active immunity is long-lasting, and sometimes life-long.Vaccines are available for all of the following vaccine-preventable diseases Typhoid hepatitis A and B influenza and many more
b)Passive Immunity
It is provided when a person is油given油antibodies to a disease rather than producing them through his or her own immune system. A newborn baby acquires passive immunity from its mother through the placenta. A major advantage of passive immunity is protection is mediated whereas active immunity takes time usually 2 to 3 weeks but it is for only few weeks for months only active immunity is long lasting e.g. tetanus Pertussis Diphtheria etc. is cure by passive immunity
2.Diagnosis
It use purified antibody solutions (antiserum) to diagnose disease. Diagnostic antibodies can be produced to detect particular microbe
A. In animals (mixed antiserum)
Inject animal with microbe or antigenic fragments
Allow immune response (1-2 weeks)
Harvest blood
Purify antibodies from serum to make antiserum = purified antibody solution to one particular antigen. These preparations will produce multiple Antibody types that recognizeelisa_Basic.pptx
elisa_Basic.pptxJingFongEwe1
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ELISA is a sensitive and specific biochemical assay that uses antibodies and enzymes to detect and quantify the presence of antigens or antibodies in a sample. It involves immobilizing an antigen or antibody on a solid surface, adding a primary antibody, and detecting with a secondary antibody conjugated to an enzyme. There are different types of ELISA including sandwich, competitive, and reverse based on whether antigens or antibodies are detected. ELISA has wide applications in research, clinical diagnostics, and drug development such as detecting infectious agents, monitoring vaccines/therapies, and screening drug candidates.ELISA
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These slides made by Miss Khunsha Fatima. This presentation will cover mainly ELISA, its Basic Principle, Steps and its related topics discussed in detail.IMMUNOLOGY.pptx
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Natural killer (NK) cells are a type of lymphocyte that plays a major role in the innate immune system by killing tumor cells and virally infected cells. NK cells are cytotoxic and contain granules with proteins like perforin and granzymes that can induce apoptosis in target cells. Patients deficient in NK cells are highly susceptible to herpes virus infections. NK cells recognize and kill tumor or infected cells but can also communicate signals to block destruction of tumors or viruses depending on the signals they receive. They are activated through killer activating receptors. [END SUMMARY]Serological test for fungi
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Serological test for fungi- Immunodiffusion, RAST, Latex Agglutination test, , Complement fixation test, ELISALary nel b. abao t. gondii and n. caninum reports
Lary nel b. abao t. gondii and n. caninum reportsObihiro University of Agriculture and Veterinary Medicine
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The document summarizes an experiment using an ELISA test with recombinant NcSAG1 to detect antibodies for Neospora caninum in canine serum samples. Key points:
- The indirect ELISA method was used to test 12 canine serum samples for N. caninum antibodies. This method has advantages like increased sensitivity but requires an extra incubation step.
- OD values indicated samples 1, 4, and 3 had the highest antibody levels while samples 9, 6, and 2 had the lowest.
- Based on the OD values, samples 1, 3, 4, 5, 7, 8, 10, 11, and 12 tested positive for N. caninum antibodies while 2,Detection of Pathogenic Strains of Entamoeba Histolytica in Children Using EL...
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Lary nel b. abao t. gondii and n. caninum reports
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Proposal march 2012
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This study aims to analyze conserved amino acids in GAPDH proteins from tropical plants Oxalis corniculata and Plectranthus amboinicus. GAPDH is important for energy production. Previous work cloned GAPDH genes from these plants and Myrtaceae psidium. Psidium showed no cloning and was eliminated. The current work will sequence the cloned GAPDH inserts and analyze conserved amino acids related to catalytic function through bioinformatics. This adds to knowledge of important plant genes and their evolution.Valeria proposalsat
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This document summarizes a study on cloning and analyzing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes from different plant species. The researcher amplified GAPDH genes from Oxalis corniculata, Plectranthus amboinicus, and Myrtaceae psidium via PCR. They then cloned the genes into E. coli and sequenced them using Sanger sequencing. The goal was to compare the GAPDH sequences to analyze conserved amino acids in the active site and differences elsewhere, helping understand GAPDH protein function and evolution across plants. Preliminary results were obtained but not detailed.Valeria riverafinalpresentationdec2011v11 2
Valeria riverafinalpresentationdec2011v11 2valrivera
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The author cloned GAPDH genes from Oxalis corniculata and Plectranthus amboinicus plants and conducted bioinformatics analyses. GAPDH is important for energy production. The genes were amplified by PCR and sequenced. Sequence analyses showed the cloned genes were GAPC from O. corniculata and GAPDH from P. amboinicus. Future work will include more detailed bioinformatics analyses and submitting the novel sequences to GenBank.Workshop oct 7 9,2010 pic
Workshop oct 7 9,2010 picvalrivera
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The document announces a nanotechnology workshop from October 7th to 9th organized by Ms. Ivonne Ferrer and Ms. Jesus Velazquez. The workshop will likely focus on nanotechnology topics over the three day period and is being run by the two individuals mentioned.Biol 3009 resumenes
Biol 3009 resumenesvalrivera
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This document summarizes the speaker's experience in three biology courses at college. In BIOL 3009, the speaker learned about the central dogma of biology including DNA replication, transcription of DNA to RNA, and translation of RNA to proteins. They enjoyed conducting a laboratory experiment to identify fruits containing enzymes. In BIOL 3095, the speaker learned to write scientific papers and presented a review paper on osteoarthritis treatments. They attended seminars by alumni researchers. In BIOL 3097, the speaker dissected samples under microscopes, extracted their own DNA, and conducted research with a mentor on GMOs, presenting their findings. They viewed the hands-on experiences as unique and fun opportunities to learn.Reflective essay blog
Reflective essay blogvalrivera
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The student reflects on their experience in the RISE program at UPR-Cayey. They took biology courses where they conducted laboratory work and wrote proposals. This helped them think like a scientist by asking questions and searching for information. They attended RISE seminars that introduced them to new scientific terms and potential research opportunities. This led the student to be selected for a summer research program at the University of Minnesota where they wrote annotated bibliographies and learned to communicate scientific findings. In their second semester, the RISE biomedical techniques course taught them new lab techniques to help achieve their career goals in the sciences.Lab 11 e
Lab 11 evalrivera
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Beer's law states that absorbance is directly proportional to concentration, allowing this lab to use a spectrophotometer to calculate protein levels in milk samples. The students prepared 1:50 dilutions of evaporated and fresh milk with dye, incubated for 40 minutes, and measured absorbance at 595 wavelengths to generate a standard curve and determine that sample A, evaporated milk, had a higher protein concentration than the fresh milk.Lab 10 e
Lab 10 evalrivera
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Enzymes are proteins that act as catalysts and have specific shapes that determine their substrates and products. The experiment determined the optimum temperature of the enzyme cellobiase, which breaks down cellulose, to be 22属C. Samples of cellobiase were tested at 0属, 22属, and 37属C using a spectrophotometer to measure absorbance, finding that the enzyme works best at 22属C and its activity is minimized at higher temperatures due to denaturing.Lab 9 e
Lab 9 evalrivera
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During a lab experiment, students created a hydrogen sensor using palladium-coated polymer nanofibers produced through electrospinning and sputter coating, which was then tested in a chamber with controlled hydrogen and argon gas flows. The sensor worked effectively, with an increase in current detected as hydrogen levels rose, demonstrating an application of nanotechnology to increase surface area and catalytic reactivity for hydrogen detection.Autobiography val
Autobiography valvalrivera
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Valeria Rivera was born on December 17, 1992 in San Juan, Puerto Rico weighing 5 pounds and 10 ounces. She enjoys arts and crafts, knitting, bicycling, jogging, visiting beaches and swimming. She graduated high school with high honors in 2010 and is interested in studying veterinary medicine, having done volunteer work at a veterinary clinic.Presentation2
Presentation2valrivera
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This document discusses phase contrast cheek cells at different magnifications. It contains images of cheek cells at 100X, 400X, and 200X magnifications from a microscope, as well as images found on the internet of cheek cells.Valeria1st labmicroscopylv
Valeria1st labmicroscopylvvalrivera
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This document summarizes a first lab on microscopy led by Dr. Robert Ross. It discusses how Robert Hooke coined the term "cell" from his observations of dead plant tissue under a microscope. The lab included a video using a scanning electron microscope to show the detailed internal structure of corn, from the grain down to starch granules. Students were then divided into groups to examine different cloth fibers under microscopes and determine that animal fibers were looser than synthetic fibers due to trapped air.Valeria laboratory 5lv
Valeria laboratory 5lvvalrivera
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DNA profiling or fingerprinting uses DNA samples to distinguish between individuals of the same species, which involves preparing DNA samples with enzymes, digesting the samples, separating DNA pieces by size using electrophoresis, and visualizing and comparing bands to identify suspects at a crime scene. The laboratory also involved using bioinformatics databases from NCBI to search for species, check DNA sequences, and compare sequences to determine the most similar species, which is important for understanding diseases and identifying new targets for pharmaceuticals through genomic information.Valeria laboratory 4lv
Valeria laboratory 4lvvalrivera
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During a weekend workshop at the University of North Carolina at Chapel Hill, graduate students extracted their own DNA from mouth cells and observed it under a microscope. They also used polymerase chain reaction (PCR) to copy and amplify specific DNA segments from samples to compare and analyze results. Finally, the students conducted electrophoresis to separate DNA fragments or proteins by size, shape, and charge, allowing them to visualize DNA bands moving toward a positive electrical pole due to the molecule's negative charge.Valeria lab 3lv
Valeria lab 3lvvalrivera
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Lab #3 describes the procedure for water sampling and testing, including taking the sample, transporting and storing it, and analyzing for E. coli and total coliforms using a substrate and incubating in a Quanty-Tray. The lab also involves using the collected data in Excel to calculate statistics like the mean, average, standard deviation, and determining that sample #2 tested positive for total coliforms at 231 MPN and E. coli at 2 MPN.Proposal rise written ok (2)
Proposal rise written ok (2)valrivera
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The document proposes an experiment to test whether a tea made from apple and lettuce produces a calming effect in hyperactive dogs. Sixty dogs will be divided into three groups - one group will receive the tea, one a commercial anxiety medication, and one a negative control. The dogs' behavior will be observed during weekly obedience tests to see if the tea makes them calmer and easier to train. If results support the hypothesis, the tea could be developed as a natural calming product for dogs.Presentation gmo (2)
Presentation gmo (2)valrivera
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This document summarizes an experiment that aimed to detect the presence of genetically modified organisms (GMOs) in various food items (papaya, soy milk, wheat, and corn) using polymerase chain reaction (PCR) and gel electrophoresis. DNA was extracted from the food samples and amplified using PCR with primers for the CaMV35S and NOS promoters/terminators typically found in GMOs. The results of running the PCR products on a gel were inconclusive, possibly due to human errors. The researchers plan to repeat the experiment to improve their results and determine which local Puerto Rican products contain GMOs.Lab 12 lv
- 1. Lab # 12: ELISA Immuno Explorer Dr. Ricardo Chiesa The purpose of this laboratory is to determine who has been exposed to a contagious disease and track the disease back to its source. For this, we used ELISA that simulates the sharing of body fluids. ELISA relies on antibodies to detect the presence of a disease (antigens) in liquid samples. Antigens result from an immune response that the body mounts when exposed to an antigen. Antibodies are proteins that recognize the antigen and bind to it; each antibody recognizes a single antigen. First students shared their samples randomly followed by ELISA. That involved putting primary and secondary antibodies to determine who has the disease and who transferred the disease. Our results demonstrated that students 1, 2, 8, and 10 were infected with the disease whereas students 1 and 10 transmitted the disease. In the real world ELISA is used for pregnancy tests, disease detection in plants and animals, detecting illegal drug use, and testing air quality. Also it is applied in tests for bacteria food safety and for genetically modified organisms (GMOs).