Lesniak t-2 toxin

Oct 15, 20160 likes63 views

The document contains two figures showing the effects of T-2 toxin and quercetin on rabbit ovaries. Figure 2 shows that T-2 toxin decreases the number of proliferating cells in the ovarian stroma, while quercetin prevents this decrease when administered together with T-2 toxin. Figure 3 shows that T-2 toxin increases the number of apoptotic cells in the ovaries, primarily in early stage follicles, while quercetin reduces apoptotic cells when combined with T-2 toxin.

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Figure 2. Effect of T-2 toxin and quercetin on number of proliferating
cells in the ovarian stroma. Each value represents the mean ±
SEM from 5 rabbits; values with different superscript letters differ
p < 0.05)
Cell apoptosis
Figure 3. Immunohistochemical localization of TUNEL-positive cells in rabbit ovary after T-2 toxin and quercetin treatment. A, B, E, F, I, J, M, N –
apoptotic cells mainly presented in the primordial follicles (thin arrows) and granulosa layer of the primary and secondary follicles (thick arrows).
C, G, K, O – numerous apoptotic cells in the preantral and antral follicles. D, H, L, P – abundant apoptotic cells in the granulosa of preovulatory
follicles. Symbols: a – antrum, at – atretic follicle, g – granulosa, o – oocyte, s – stroma, t – theca. Scale bar: 50
Control T-2 Quercetin T-2 + Quercetin
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Lesniak t-2 toxin

4. 267 Control T-2 Quercetin T-2 + Quercetin 10 9 8 7 6 5 4 3 2 1 0 a b b b ac No.ofproliferatingcells Figure 2. Effect of T-2 toxin and quercetin on number of proliferating cells in the ovarian stroma. Each value represents the mean ± SEM from 5 rabbits; values with different superscript letters differ p < 0.05) Cell apoptosis Figure 3. Immunohistochemical localization of TUNEL-positive cells in rabbit ovary after T-2 toxin and quercetin treatment. A, B, E, F, I, J, M, N – apoptotic cells mainly presented in the primordial follicles (thin arrows) and granulosa layer of the primary and secondary follicles (thick arrows). C, G, K, O – numerous apoptotic cells in the preantral and antral follicles. D, H, L, P – abundant apoptotic cells in the granulosa of preovulatory follicles. Symbols: a – antrum, at – atretic follicle, g – granulosa, o – oocyte, s – stroma, t – theca. Scale bar: 50 Control T-2 Quercetin T-2 + Quercetin No.ofapoptoticcells 10 9 8 7 6 5 4 3 2 1 0 T-2toxinT-2toxinQuercetinControl Quercetin

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Lesniak t-2 toxin

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