My resume
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This document contains the resume of Amol Martandrao Kulkarni. It summarizes his contact information, career objective, areas of expertise, professional experience, and educational qualifications. His areas of expertise include extensive experience conducting in vitro and in vivo anti-infective evaluations, developing resistance to antibiotics, and using molecular biology techniques like PCR. He is currently working as a Senior Research Associate focusing on developing new broad spectrum antibacterial agents.
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My resume
- 1. Contact No: 9405367843 / 9028729272 Email: kool.amol@gmail.com Amol Martandrao Kulkarni C/o P. Y. Kulkarni, Flat no 1, Anuyog Apartment, Nandigram Colony, Garkheda, Aurangabad-431005, Maharashtra, India As an experienced researcher so far; I would like to be a reason for the development and advancement of the esteemed organization in which I would be working, using my skills, expertise and learning to the best of my capability to raise the firm as well as myself to a new level which would also satisfy my soul to be in research. In vitro anti-infective evaluation: Extensive hands on experience in microbiological evaluation of various NCEs from variety of classes of antibacterial such as fluoroquinolones, oxazolidinones, macrolides and ketolides. Drug diffusion assays in agar media. Spectrum determination by Minimum Inhibitory Concentration (MIC) determination by agar and broth dilution method. Modified agar dilution method for MIC determination in 96-culture format using lids of 96 well microtitre plates to generate extensive data. MIC determination for anaerobe cultures by using anaerobic chamber Performing biofilm assays for Pseudomonas and Staphylococcus genera Time kill studies for diverse pathogens Determination of resistance selection window Career Objective ive: Area of Expertise ive:
- 2. Contact No: 9405367843 / 9028729272 Email: kool.amol@gmail.com Enzyme inhibition assays (Determination of IC50) In-vitro resistance development: To develop an In vitro resistance to differentiate the activities of NCEs and antibacterial agents used in the clinics. Molecular Biology Techniques: Genotyping by using PCR Isoelectric focusing (IEF) of ESBL PFGE SDS-PAGE In vivo expertise Extensive hands on experience in in vivo evaluation of various NCE s from variety of classes of antibacterial such as fluoroquinolones, oxazolidinones, macrolides, ketolides and beta lactams 鐃 Animal handling and experimentation on mice, rat, rabbits and guinea pigs, etc. 鐃 Viable counts of bacilli in organs of animals. 鐃 In vivo efficacies of drugs against bacterial infections. 鐃 Experience in development of animal models for basic screening of NCE鐃s against variety of bacterial pathogens, such as: 1. Mouse peritonitis model 2. Staphylococcal septicemia 3. Pyelonephritis (UTI model) 4. Thigh infection 5. Skin abscesses 6. Bacterial endocarditis 7. Lung infection model 8. Tissue burden models 9. Bone and Joint Infection model
- 3. Contact No: 9405367843 / 9028729272 Email: kool.amol@gmail.com Currently working as a Senior Research Associate (New Drug Discovery department) at Wockhardt Research Center, Maharashtra, India since December 2008. The work in which I am involved is all about developing a new broad spectrum antibacterial agents against MDR bacterial infections. Wockhardt Research Centre Dec 2008 Till Date Aurangabad, Maharashtra Current Designation Senior Research Associate (New Drug Discovery department) Key Roles & Responsibilities In-vitro studies: -Primary screening of NCEs: To screen the New Chemical Entities (NCEs) at primary level by using Agar dilution MIC method for their Activity against clinical isolates harboring various resistance mechanisms. To compare the activity of NCEs with that of antibacterial agents used in clinics and which are under Development. -Understanding the Resistance mechanism to antibiotics: To study and understand the different mechanisms of resistance in bacterial clinical isolates. -In-vitro resistance development: To develop an In vitro resistance to differentiate the activities of NCEs and antibacterial agents used in the Clinics. Professional Experience ive:
- 4. Contact No: 9405367843 / 9028729272 Email: kool.amol@gmail.com In-vivo studies: -Animal handling -Inducing/developing Infections and dosing through various routes such as: Intravenous Intraperitoneal Intramuscular Intranasal Oral Subcutaneous -In-vivo screening of NCEs Systemic infection studies Organ Eradication Studies (Lung, Kidney, Thigh) -Development of New Murine Infection Models Philosophy of Doctorate (PhD) Pursuing PhD in the subject of clinical Microbiology from Swami Ramanand Teerth marathwada University, Nanded, Maharashtra. with the focus on Studies on development of resistance to Fluoroquinolones in Gram negative Bacterial Pathogens. Submitted the final synopsis and going to submit the thesis soon. Master of Science in Microbiology (M.Sc.) July2008 Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, India. Educational Qualification ive:
- 5. Contact No: 9405367843 / 9028729272 Email: kool.amol@gmail.com Bachelor of Science in Microbiology, Chemistry and Botany (B.Sc. ) June2006 Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra, India. Senior secondary education May2002 Maharashtra Divisional Board of higher secondary Education, Maharashtra. Dr. Mahendra K. Ranjekar Prof. in biology, Dept. of Applied Sciences, College of Engineering, Pune #5, Maharashtra (India) E-mail ID: mkr.appsci@coep.ac.in Mob. : 09422015217 I hereby declare that the information given by me in this resume is true and up to date to the best of my knowledge. DATE: PLACE: Aurangabad (Maharashtra) Recommended by