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Introduction/Academic Background:
• My name is Muhammad Ali Assad, and I am from Pakistan, having an Master of Philosophy (M.Phil.) degree
in Epidemiology and Public Health with a CGPA of 3.76/4.0 from University of Veterinary and Animal
Sciences Lahore (UVAS).
• Graduated with degree of Doctor of Veterinary Medicine (DVM) from University of Veterinary and Animal
Sciences Lahore (UVAS).

Professional Experience:
• Veterinary Teaching Hospital (UVAS).
• Experimental Broiler Farm affiliated with (UVAS).
• Pre internship, Poultry Diagnostic Lab affiliated with (UVAS).
• Final internship, SABIR'S POULTRY, (Broiler Breeder farming).
• Assistant farm & farm manager at Broiler farm.
• Assistant farm manager at a commercial layer farm.

Research Experience:
Participated in Following research Projects:
• Assessment of knowledge attitude and practices to- wards antimicrobial resistance (AMR) among the livestock workers in
district Layyah. (MPhil Thesis)
• KAP Survey on Zoonotic Diseases among Livestock farmers in district Muzaffargarh.
• Seroprevalence and risk factors of Tuberculosis in the District Hospital of Jhang.
• Experiment on seroprevalence of Brucellosis among animals visiting CVAS Teaching Hospital.
• Prevalence estimation on samples collected from a suspected brucellosis-infected cow herd.
• Serological investigation of Chlamydia abortus in small ruminants.
• Oxidative stress and Genotoxicity assessment due to sub-acute administration of chlorpyrifos a chemical used in fertilizers
in male rabbits.
• Effect various types of housing systems in poultry especially broilers.

Research Experience:
Participated in Following Research Diagnostic Tests/Procedures:
• Sample collection, transport, handling, storage, tagging and maintenance.
• Complete Blood Counts (CBC).
• Bacterial culture isolation and identification.
• Antimicrobial susceptibility tests.
• Independent & Supervised postmortem and diagnosis of Poultry.
• Milk Ring Test.
• The Rose Bengal test (RBT).
• Use of Serum-indirect Elisa.
• Quantitative PCR (qPCR).
• Use of Gel Electrophoresis.
• PCR in molecular diagnostics.

PhD Study Plan:
PhD Plan:
Choosing to pursue a PhD in a lab specializing in advance molecular diagnostic and understanding of public health
challenges, like avian influenza, in disease mechanisms and host-pathogen interactions. Such labs provide access to
cutting-edge tools, expertise in interdisciplinary opportunities, enabling groundbreaking work in vaccine
development, gene regulation, and therapeutics. This specialization offers the ideal platform for impactful research
and career development in molecular epidemiology and public health.
Key PhD Research Directions:
 Molecular Surveillance of Avian Influenza Virus
 Host-Virus Interaction Mechanisms
 Cross-Species and Zoonotic Implications
Personal Background:
I am deeply motivated to address global health challenges by contributing to the understanding, prevention, and
control of zoonotic diseases. My academic and research background has equipped me with strong foundations in
epidemiology, molecular biology, and infectious disease control. My focus on avian influenza reflects the urgent need
for multidisciplinary approaches to mitigate its impact on animal and human populations.

PhD Study Plan:
Objectives of Study:
 To study the molecular epidemiology and public health implications of avian influenza, with a particular focus on
understanding viral evolution, transmission dynamics, and immune responses.
 Develop robust diagnostic tools and molecular assays for early detection of avian influenza strains.
 Characterize genetic markers associated with pathogenicity and interspecies transmission.
 Explore immune response pathways and evaluate vaccine efficacy.
 Conduct epidemiological modeling to assess the public health impact and develop control strategies.
Anticipated Outcomes:
 A deeper understanding of the genetic and molecular mechanisms driving avian influenza evolution and spread.
 Development of innovative diagnostic tools for early detection and characterization of AIV.
 Policy recommendations for improving surveillance systems and vaccination strategies to prevent outbreaks.
Significance of Study:
 This research will contribute to:
 Bridging knowledge gaps in molecular epidemiology and public health.
 Enhancing global preparedness for avian influenza outbreaks.
 Informing vaccine development and zoonotic disease prevention strategies.

Study Plan Timeline:
Year Milestones/Activities Details
Year 1 Foundation & Planning
Literature Review Conduct in-depth review of existing studies on research topic.
Define Objectives Formulate specific, measurable research questions and hypotheses.
Methodology Design Design experimental and analytical approaches for the project.
Coursework Complete required courses on epidemiology, biostatistics, molecular techniques, etc.
Collaboration Building Network with supervisors, peers, and attend workshops or initial conferences.
Proposal Approval Write and defend the research proposal.
Year 2 Data Collection & Experiments
Laboratory/Field Work Collect data, perform lab-based molecular tests, and ensure sample quality and quantity.
Data Management Organize data collection, storage, and initial analyses for integrity.
Interim Reports Submit regular progress updates to the advisory committee for feedback.
Conference Participation Present preliminary findings at workshops or conferences.
Year 3 Data Analysis & Writing
Advanced Data Analysis Perform statistical analysis, bioinformatics, and interpretation of data.
Thesis Writing Write and revise the dissertation (Introduction, Methods, Results, Discussion)
Publications Prepare and submit research articles to peer-reviewed journals.
Defense Preparation Finalize dissertation, attend mock defenses, and prepare for oral defense.

Publications:
• Effect various types of housing systems in poultry especially broilers
DOI: https://doi.Org/10.70749/ijbr.V2i02.254
• Effect of yeast as prebiotics in small and large ruminants diet.
Doi:10.5281/zenodo.14 169524
• Effect of heat stress on physiological behaviour in dairy animals and poultry
Issn: 3007-1208 | 3007-1216
• Basic insights into lymphatic filariasis” book chapter .
Https://uniquescientificpublishers.Com/wp-content/uploads/2023/zon-v2/73-88.Pdf
• Revolutionizing nanoparticles in veterinary care: classifications and benefits” book chapter.
Https://uniquescientificpublishers.Com/wp-content
• Application of nanoparticles against salmonellosis” book chapter.
Https://empyrealpublishinghouse.Com/
• Impact of zika virus on pregnant women” abstract published in one health international conference
booklet held at (UVAS)
Https://jassaraftab.Com/wp-content/uploads/2022/12/abstract-book-ohc-2022.Pdf
• Prevalence of brucellosis and its risk factors among animals visiting teaching hospital of (CVAS)
abstract published in one health international conference booklet held at (UVAS)
Https://uvas.Edu.Pk/NEWS/2021/final%20abstract%20book%20ohc%2011-12-2020.Pdf

Conferences, Seminars and Workshops:
• One Health International Conference (OHIC-2018) at UVAS.
• One-day workshop on "Research Proposal Writing and Intellectual Property Rights"
• One-day "Free Hepatitis Screening & Vaccination Camp"
• One-day workshop on "Hands-on Training in Hematological Analysis in Domestic Animals"
• One-day webinar about "North American Veterinary Licensing
• One Health International Conference (OHIC-2022) at UVAS.
• On-day workshop on Biosafety, Bio risk Management and Responsible Science.
• One Day Workshop on Genomic DNA Extraction and PCR in Molecular Diagnostics at UVAS.
• Workshop on "Hands-on Training in Hematological Analysis in Domestic Animals" at UVAS.

Future Plans:
• Vaccine Development for Avian Influenza.
• Universal Avian Influenza Vaccines.
• AVI seroprevalence.

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