Physiology Retreat 2016 - Blitz- Shayan
Download as PPTX, PDF
0 likes61 views
miR146a is a microRNA that regulates inflammation and is being investigated for its potential role in lipids metabolism and atherosclerosis. PCSK9 gain of function mutations cause high cholesterol by reducing LDL receptors, resulting in increased total cholesterol levels. The document discusses exploring the role of miR146a in the mechanism by which PCSK9 causes hypercholesterolemia.
Physiology Retreat 2016 - Blitz- Shayan
- 1. miR146a is a microRNA Well known to regulate Inflammation Injecting PCSK9 AAV vector to mimic high cholesterol Where to go: miR146a and lipids metabolism Effect on atherosclerosis PCSK9 gain of function mutation results in increased cholesterol by reducing LDL receptors TotalCholesterol(mg/dl) 0 50 100 150 200 250 WT KO Plasma Cholesterol PCSK9 mechanism of action on hypercholesterolemia: possible role of miR146a Shayan Mohamamdmoradi Dr. Subramanian Laboratory Investigating role of miR146a * Baseline Week 4 *P<0.05 WT vs KO
Editor's Notes
- PCSK9 is an enzyme that its GOF mutation will result in increased cholesterol. Also, miR146a is a microRNA that seems to play a role in cholesterol efflux. In this project, we investigated the role of miR146a in PCSK9 induced hypercholesterolemia. Using miR146a WT and KO mice, miR146a KO mice showed a significant reduction of plasma total cholesterol, inducing HDL-C levels irrespective to comparable PCSK9 levels. Our current focus is to understand the mechanism i.e. identifying its targets by which miR146a regulates HDL-C and its effect on atherosclerosis development.