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easyEST                                  MT
                             Automation-Compatible
                      Embryonic Stem Cell Toxicity Test
Toxicology Products




                                     損 Simpler and much more reliable embryoid-
                                       body production
                                     損 No manual turning of culture dishes
                                     損 Direct transfer of EBs  fast, easy and no
                                       manual picking!
                                     損 Easy scale-up of EB production
What is the EST?
The Embryonic Stem Cell Test (EST) is an assay used to evaluate embryotoxicity and
teratogenicity of compounds, cosmetics and chemical applications. The European
Centre for the Validation of Alternative Methods (ECVAM) has validated an EST
protocol, which is widely used. The EST requires the production of mouse
embryoid bodies (EBs) by the classic hanging-drop method which is generally
viewed as a time-consuming, labour-intensive and delicate process. After formation
the EBs are hand-picked and transferred into a multiwell plate for differentiation
into beating cardiomyocytes.




How does InSpheros easyESTTM make the test more efficient?
InSpheros easyEST kit* is based on InSpheros patent-pending culture technology,
which allows the formation of 96 hanging drops by top loading into the plate. No
turning of the culture dish is necessary and the formed EBs can easily be trans-
ferred into a 96-well culture plate for the downstream assay. The easyEST system
is compatible with every multi well-compliant liquid handling device ranging from
a manual single channel pipette to a robotic 96-tip dispenser. The easyEST process
is extremely reliable and time saving, and finally makes your EST fully automation
compatible.
* The easyEST system is not yet validated by ECVAM
And the beat
    goes on                                                    Advantages of InSpheros easyEST

                        Faster and easier: No manual turning of the culture dish  top loading into the easyEST system
                        directly forms stable hanging drops.

                        No manual picking: Cultured EB can be directly transferred into a 96-well plate for your downstream
                        assay, no time-consuming manual picking involved.

                        Automation compatible: The easyEST system can be loaded by any liquid-handling equipment,
                        robotic, semi-automated or manual.

                        Highly reproducible: The precise drop-formation process in the easyEST system assures a size variation
                        below 10% and makes your EST much more robust.




 息karandaev - Fotolia



                                                                                                        www.insphero.com
Services Overview
                                                                                                                   For feasibility studies or training for
                                                                                                                   using our easyEST system please
                                                                                                                   contact our sales department at
                                                                                                                   sales@insphero.com




                                                                                              Product Overview
easyEST kit for EB production


Cat. #                   Name                       Description
CS-01-001                easyEST kit                Hanging-drop cell culture plate, 96 wells, gamma sterilized,
                                                    with evaporation control, lid, bottom plate and flat bottom
                                                    receiver plate. For use with EST only.                                                                InSphero AG
                                                                                                                                                    Technoparkstrasse 1
                                                                                                                                                        CH-8005 Zurich
                                                                                                                                                            Switzerland
                                                                                                                                                     www.insphero.com
                                                                                                                                              Phone: +41-44-515049-0
                                                                                                                                            e-mail: sales@insphero.com

All technical and pricing information subject to change.
All products are for research use only. They are not intended for any animal
or human therapeutic or diagnostic use unless otherwise stated.

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Products toxicology est_web

  • 1. easyEST MT Automation-Compatible Embryonic Stem Cell Toxicity Test Toxicology Products 損 Simpler and much more reliable embryoid- body production 損 No manual turning of culture dishes 損 Direct transfer of EBs fast, easy and no manual picking! 損 Easy scale-up of EB production
  • 2. What is the EST? The Embryonic Stem Cell Test (EST) is an assay used to evaluate embryotoxicity and teratogenicity of compounds, cosmetics and chemical applications. The European Centre for the Validation of Alternative Methods (ECVAM) has validated an EST protocol, which is widely used. The EST requires the production of mouse embryoid bodies (EBs) by the classic hanging-drop method which is generally viewed as a time-consuming, labour-intensive and delicate process. After formation the EBs are hand-picked and transferred into a multiwell plate for differentiation into beating cardiomyocytes. How does InSpheros easyESTTM make the test more efficient? InSpheros easyEST kit* is based on InSpheros patent-pending culture technology, which allows the formation of 96 hanging drops by top loading into the plate. No turning of the culture dish is necessary and the formed EBs can easily be trans- ferred into a 96-well culture plate for the downstream assay. The easyEST system is compatible with every multi well-compliant liquid handling device ranging from a manual single channel pipette to a robotic 96-tip dispenser. The easyEST process is extremely reliable and time saving, and finally makes your EST fully automation compatible. * The easyEST system is not yet validated by ECVAM
  • 3. And the beat goes on Advantages of InSpheros easyEST Faster and easier: No manual turning of the culture dish top loading into the easyEST system directly forms stable hanging drops. No manual picking: Cultured EB can be directly transferred into a 96-well plate for your downstream assay, no time-consuming manual picking involved. Automation compatible: The easyEST system can be loaded by any liquid-handling equipment, robotic, semi-automated or manual. Highly reproducible: The precise drop-formation process in the easyEST system assures a size variation below 10% and makes your EST much more robust. 息karandaev - Fotolia www.insphero.com
  • 4. Services Overview For feasibility studies or training for using our easyEST system please contact our sales department at sales@insphero.com Product Overview easyEST kit for EB production Cat. # Name Description CS-01-001 easyEST kit Hanging-drop cell culture plate, 96 wells, gamma sterilized, with evaporation control, lid, bottom plate and flat bottom receiver plate. For use with EST only. InSphero AG Technoparkstrasse 1 CH-8005 Zurich Switzerland www.insphero.com Phone: +41-44-515049-0 e-mail: sales@insphero.com All technical and pricing information subject to change. All products are for research use only. They are not intended for any animal or human therapeutic or diagnostic use unless otherwise stated.