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Uji Antagonisme Trichoderma
harzianum Dan Azotobacter
chroococcum Terhadap Rhyzoctonia
solani, Sclerotium rolfsii dan
Fusarium oxysporum secara in-vitro
By 13 有 悠 Group :
Muhammad Ilham Jasir (24020118120028)
Uji Antagonisme Trichoderma harzianum Dan Azotobacter chroococcum.pptx
Penelitian ini bertujuan mengkaji daya
antagonisme T. harzianum dan A. chroococcum
yang terdapat pada carrier bahan organik padat
TRIAZOTE yang telah disimpan selama lima
bulan terhadap fitopatogen R. solani, S. rolfsii,
dan F. oxysporium secara in vitro.
BAHAN DAN METODE
 Menggunakan inokulan T. harzianum dan A.
chroococcum, yang ditumbuhkan secara bersama-sama
pada media bahan organik padat berbasis kompos
limbah olahan minyak kayu putih dan ela sagu
(TRIAZOTE) yang telah disimpan pada suhu ruang
dengan minim cahaya selama lima bulan.
 Biakan murni inokulan Rhizoctonia solani, Sclerotium
rolfsii, dan Fusarium oxysporum yang diisolasi dari
tanaman sakit dan merupakan koleksi Laboratorium
Penyakit Tumbuhan Faperta Unpatti
TAHAP
1) Perlakuan yang dicobakan adalah menguji antagonisme T. harzianum dan
A. chroococcum masing-masing terhadap pertumbuhan patogen R.
solani, S. rolfsii, dan F. oxysporum.
2) Inokulan T. harzianum dan A. chroococcum diisolasi dari 1 g TRIAZOTE
dihaluskan kemudian ditambahkan dengan 9 ml air steril, dikocok
dengan fortex selama 5 menit pada media PDA modifikasi
3) Inokulan T. harzianum pada media PDA diambil menggunakan corer
borer  9 mm, sedangkan inokulan A. chroococcum pada media Asby di
tabung reaksi,
4) Pengujian daya antagonisme masing- masing T. harzianum dan A.
chroococcum terhadap patogen R. solani, S. rolfsii, dan F. oxysporum
menggunakan metode dual kultur pada media PDA
5) Pengukuran daya antagonisme A. chroococcum terhadap S. rolfsii dan F.
oxysporum dihitung dengan cara mengukur luas koloni S. rolfsii dan F.
oxysporum yang ditumbuhkan tanpa A. chroococcum (kontrol)
dibandingkan dengan luas koloni S. rolfsii dan F. oxysporum yang
ditumbuhkan bersama dengan A. chroococcum
HASIL DAN PEMBAHASAN
Trichoderma hazianum berpengaruh secara signifikan
terhadap pertumbuhan patogen R. solani, S. rolfsii, dan F.
Oxysporum.
Persentase hambatan pertumbuhan R. solani (54,57%), S.
rolfsii (66,22%) dan F. oxysporum (68,57%).
Hal ini menunjukan bahwa masing-masing jenis patogen
memberikan respons yang berbeda terhadap serangan
agen hayati T. harzianum dengan berbagai mekanisme
Azotobacter chroococcum menekan pertumbuhan patogen R. solani, S.
rolfsii, dan F. oxysporum.
Azotobacter chroococcum tidak memberikan pengaruh secara
signifikan terhadap pertumbuhan patogen R. solani, S. rolfsii, dan F.
Oxysporum
Persentase hambatan pertumbuhan R. solani sebesar 11,02% , S. rolfsii
(9,09%) dan F. oxyprorum (10,99%).
disebabkan oleh zat atau senyawa antijamur yang dihasilkan oleh A.
chroococcum. Azotobacter merupakan rizobacter yang dapat
menghambat pertumbuhan fitopatogen melalui mekanisme aktivitas
metabolit sekunder dan zat anti mikroba yang memiliki aktivitas
antagonis [
KESIMPULAN
 Jamur T. harzianum di dalam carrier bahan organik
padat TRIAZOTE yang telah disimpan selama lima
bulan, secara signifikan menghambat pertumbuhan
patogen R. solani, S. rolfsii, dan F. oxysporum.
 Trichoderma harzianum dapat menghambat
pertumbuhan F. oxysporum sebesar 68,57%, S. rolfsii
(66,22%) dan R. solani (54,57%). Sedangkan A.
chroococcum tidak secara signifikan menghambat
pertumbuhan patogen R. solani, S. rolfsii dan F.
oxyprorum, masing-masing sebesar 11,02%, 9,09% dan
10,99%.
Thanks You!
ANY QUESTIONS?

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Uji Antagonisme Trichoderma harzianum Dan Azotobacter chroococcum.pptx

  • 1. Uji Antagonisme Trichoderma harzianum Dan Azotobacter chroococcum Terhadap Rhyzoctonia solani, Sclerotium rolfsii dan Fusarium oxysporum secara in-vitro By 13 有 悠 Group : Muhammad Ilham Jasir (24020118120028)
  • 3. Penelitian ini bertujuan mengkaji daya antagonisme T. harzianum dan A. chroococcum yang terdapat pada carrier bahan organik padat TRIAZOTE yang telah disimpan selama lima bulan terhadap fitopatogen R. solani, S. rolfsii, dan F. oxysporium secara in vitro.
  • 4. BAHAN DAN METODE Menggunakan inokulan T. harzianum dan A. chroococcum, yang ditumbuhkan secara bersama-sama pada media bahan organik padat berbasis kompos limbah olahan minyak kayu putih dan ela sagu (TRIAZOTE) yang telah disimpan pada suhu ruang dengan minim cahaya selama lima bulan. Biakan murni inokulan Rhizoctonia solani, Sclerotium rolfsii, dan Fusarium oxysporum yang diisolasi dari tanaman sakit dan merupakan koleksi Laboratorium Penyakit Tumbuhan Faperta Unpatti
  • 5. TAHAP 1) Perlakuan yang dicobakan adalah menguji antagonisme T. harzianum dan A. chroococcum masing-masing terhadap pertumbuhan patogen R. solani, S. rolfsii, dan F. oxysporum. 2) Inokulan T. harzianum dan A. chroococcum diisolasi dari 1 g TRIAZOTE dihaluskan kemudian ditambahkan dengan 9 ml air steril, dikocok dengan fortex selama 5 menit pada media PDA modifikasi 3) Inokulan T. harzianum pada media PDA diambil menggunakan corer borer 9 mm, sedangkan inokulan A. chroococcum pada media Asby di tabung reaksi, 4) Pengujian daya antagonisme masing- masing T. harzianum dan A. chroococcum terhadap patogen R. solani, S. rolfsii, dan F. oxysporum menggunakan metode dual kultur pada media PDA 5) Pengukuran daya antagonisme A. chroococcum terhadap S. rolfsii dan F. oxysporum dihitung dengan cara mengukur luas koloni S. rolfsii dan F. oxysporum yang ditumbuhkan tanpa A. chroococcum (kontrol) dibandingkan dengan luas koloni S. rolfsii dan F. oxysporum yang ditumbuhkan bersama dengan A. chroococcum
  • 6. HASIL DAN PEMBAHASAN Trichoderma hazianum berpengaruh secara signifikan terhadap pertumbuhan patogen R. solani, S. rolfsii, dan F. Oxysporum. Persentase hambatan pertumbuhan R. solani (54,57%), S. rolfsii (66,22%) dan F. oxysporum (68,57%). Hal ini menunjukan bahwa masing-masing jenis patogen memberikan respons yang berbeda terhadap serangan agen hayati T. harzianum dengan berbagai mekanisme
  • 7. Azotobacter chroococcum menekan pertumbuhan patogen R. solani, S. rolfsii, dan F. oxysporum. Azotobacter chroococcum tidak memberikan pengaruh secara signifikan terhadap pertumbuhan patogen R. solani, S. rolfsii, dan F. Oxysporum Persentase hambatan pertumbuhan R. solani sebesar 11,02% , S. rolfsii (9,09%) dan F. oxyprorum (10,99%). disebabkan oleh zat atau senyawa antijamur yang dihasilkan oleh A. chroococcum. Azotobacter merupakan rizobacter yang dapat menghambat pertumbuhan fitopatogen melalui mekanisme aktivitas metabolit sekunder dan zat anti mikroba yang memiliki aktivitas antagonis [
  • 8. KESIMPULAN Jamur T. harzianum di dalam carrier bahan organik padat TRIAZOTE yang telah disimpan selama lima bulan, secara signifikan menghambat pertumbuhan patogen R. solani, S. rolfsii, dan F. oxysporum. Trichoderma harzianum dapat menghambat pertumbuhan F. oxysporum sebesar 68,57%, S. rolfsii (66,22%) dan R. solani (54,57%). Sedangkan A. chroococcum tidak secara signifikan menghambat pertumbuhan patogen R. solani, S. rolfsii dan F. oxyprorum, masing-masing sebesar 11,02%, 9,09% dan 10,99%.