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- 1. VRINDA RAMAKRISHNAN # 9645024737 Email: vrinda.r2@gmail.com Seeking a challenging position in the field of research and development in an organization of repute. Completed PhD in Biotechnology from Central Food Technological Research Institute (CSIR-CFTRI), Mysore under the guidance of Principal Scientist, Dr. Prakash M Halami. Possess 6 publications in both national and international peer reviewed journals ( 5 as first author). Has presented 7 posters at various national and international conferences for which best poster award was won twice. 5 years of rich experience in handling Short term courses on Molecular techniques in Microbiology organized by DBT, Govt of India for Lecturers. Excellent academic record, acquired national level selection and has completed mini-project under the guidance of Dr. S Shivaji, CCMB, Hyderabad. Good team worker with excellent communication skills, sincere and committed to profession. Possess great patience under pressure, interested in learning new techniques, highly self motivated with strong work ethics. Protein extraction, purification and analysis techniques. Recombinant DNA technology. Techniques in Molecular Microbiology and molecular identification. Basic microbiological techniques including serial dilution and plating. High throughput screening and identification techniques. 1. Sound theoretical and practical knowledge of statistical softwares (Statistica OBJECTIVE PROFILE SUMMARY CORE COMPETENCIES/ SKILLS
- 2. and Design Expert) for enzyme optimization studies using Response Surface Methodology (RSM). 1. M.Sc Disssertation (in Medical Biochemistry, December 2007- March 2008) at Amala Institute of Medical Sciences under the guidance and supervision of Dr. Jose Jacob, MD in Biochemistry, Amala Hospital, Thrissur. Dissertation Title: Influence of pH on turbidity induced by Phosphotungstate Mitochondria- Lysosome enriched preparations. 2. Worked as a summer trainee for tenure of 2 months at CCMB, Hyderabad, under the guidance of Dr.Shivaji, Scientist F, CCMB. Dissertation Title: 16S rDNA approach and sequencing of psychrophilic microorganisms. 3. Has qualified CSIR-JRF 2008 amd completed PhD under Dr. Prakash M Halami, Department of Food Microbiology, CSIR-CFTRI, Mysore. 1. Combined effect of enterocin and lipase from Enterococcus faecium NCIM5363 against food borne pathogens: Mode of action studies (2012) Vrinda R, Halami PM & Bhaskar N Current Microbiology, 65 (2):162-169. 2. Concomitant production of lipase, protease and enterocin by Enterococcus faeciumNCIM5363 and Enterococcus durans NCIM 5427 isolated from fish processing waste (2012) Vrinda R, Bijinu B, Amit KR, Halami PM, Bhaskar N International Aquatic Research, 4:14. 3. Comparison of lipase production by Enterococcus faecium NCIM5363 and Pediococcus acidilactici NCIM5368: Optimization of Culture Conditions by Response Surface Methodology using fish waste as substrate (2012) Vrinda R, Louella CG, Halami PM & Bhaskar N ISRN Biotechnology, 2013:980562. RESEARCH EXPERIENCE PEER REVIEWED PUBLICATIONS
- 3. 4. Optimization of conditions for probiotic curd formulation by Enterococcus faecium MTCC 5695 with probiotic properties using Response Surface Methodology (2012) Vrinda R, Louella CG, Halami PM & Bhaskar N, Journal of Food Science and Technology, DOI 10.1007/s13197-012-0821-x. 5. Kinetic modeling, production and characterization of an acidic lipase produced by Enterococcus durans NCIM5427 using fish waste as a substrate (2013) Vrinda R, Louella CG, Halami PM & Bhaskar N, Journal of Food Science and Technology, DOI 10.1007/s13197-013-1141-5. 6. Isolation and characterization of potential Lactic Acid Bacteria (LAB) from freshwater fish processing wastes for application in fermentative utilization of fish processing waste (2010) Jini R, Swapna HC, Amit KR, Vrinda R, Halami PM, Sachindra NM & Bhaskar N, Brazilian Journal of Microbiology, ISSN 1517-8382, http://dx.doi.org/10.1590/S1517- 83822011000400039. 7. Kinetic modeling and application of lipase from Enterococcus faecium MTCC5695 and Pediococcus acidilactici MTCC 11361 (2013) Vrinda R, Louella CG, Halami PM & Bhaskar N (under process). 1. Isolation and characterization of lipolytic and proteolytic lactic acid bacteria with antibacterial activities from fish processing waste (2009) Vrinda R, Ali M, Amit AK, Halami PM & Bhaskar N, ICFOST, Bangalore. 2. Reducing disposal of solid wastes from fish processing and subsequent organic loading of environment: Isolation and characterization of a bacteriocin producing, proteolytic and lipolytic lactic acid bacteria (LAB) from fish waste (2010) Vrinda R, Bijinu B, Halami PM & Bhaskar N, IAES, MS University, Baroda. 3. Inhibition of Escherichia coli by a novel enterocin produced by the proteolytic and lipolytic lactic acid bacteria isolated from fish processing POSTERS PRESENTED AT ACADEMIC CONFERENCES
- 4. waste (2010)Vrinda R, Halami PM & Bhaskar N, 79th SBC (I) Annual Conference, Bangalore, India. 4. Optimization of conditions for lipase production by Enterococcus faecium NCIM5363 using Response Surface Methodology (2011) Vrinda R, Halami PM & Bhaskar N, AMI, Chandigarh. 5. Optimization of conditions for curd formulation by selected Lactic Acid Bacteria and analysis of the formulated product (2011) Louella CG, Vrinda R, Halami PM & Bhaskar N, SASNET, Mysore. 6. Synergistic effect of enterocin and lipase from Enterococcus faecium NCIM5363 against food borne pathogens: Mode of action studies (2012) Vrinda R, Halami PM & Bhaskar N, ICFOST, Pune (won second prize for best poster). 7. Production and characterization of an acidic lipase by Enterococcus durans NCIM5427 using fish waste as a substrate (2013) Vrinda R, Louella CG, Halami PM & Bhaskar N, ICFOST, Mysore (Best poster award). MS-OFFICE, VISUAL BASIC, C, C++, FOXPRO, MS-ACCESS. Degree/Diplom a Institute Board / University Year Of Passing Percentage M.Sc Biotechnology Sathyabama University of Science and Technology, Chennai Sathyabama University 2008 84 % B.Sc Microbiology St.Marys College, Kerala University of Calicut 2006 81 % XII th Bharatiya Vidya Bhavan, Thrissur CBSE 2003 79 % COMPUTER SKILLS ACADEMICS
- 5. Xth Bharatiya Vidya Bhavan, Thrissur CBSE 2001 86 % Molecular Biology and Genetic Engineering techniques Plant & Animal Tissue Culture Recombinant DNA Technology Bio process Engineering Immunology Environmental Biotechnology Tissue Engineering Microbial Biotechnology Received numerous prizes in literary activities both at school and college level. Member of Association of Food Scientists and Technologists, India. Name : VRINDA RAMAKRISHNAN Date of Birth : 02.09.1985 Marital status : Married Languages known : English, Hindi, Kannada, Malayalam, Tamil (1) Dr. Shivaji, Scientist F, CCMB, Hyderabad (e-mail: shivas@ccmb.res.in) CURICULLUM PROFILE EXTRACURICULAR ACTIVITIES MEMBERSHIP PERSONAL PROFILE REFERENCES
- 6. (2) Dr. Prakash M Halami , Principal Scientist., Dept. of Food Microbiology CSIR-CFTRI, Mysore, (e-mail: kash13@yahoo.co.in), #: 09448082409 (3) Dr. Bhaskar Narayan, Principal Scientist, Dept. of Meat and Marine Sciences, CSIR-CFTRI, Mysore, (e-mail: bhasnarayan@gmail.com), #:09448672408 I hereby declare that the above-furnished details are true to best of my knowledge and belief. Place: Cochin Date: 26-11-2014 Yours faithfully Vrinda Ramakrishnan DECLARATION
- 7. (2) Dr. Prakash M Halami , Principal Scientist., Dept. of Food Microbiology CSIR-CFTRI, Mysore, (e-mail: kash13@yahoo.co.in), #: 09448082409 (3) Dr. Bhaskar Narayan, Principal Scientist, Dept. of Meat and Marine Sciences, CSIR-CFTRI, Mysore, (e-mail: bhasnarayan@gmail.com), #:09448672408 I hereby declare that the above-furnished details are true to best of my knowledge and belief. Place: Cochin Date: 26-11-2014 Yours faithfully Vrinda Ramakrishnan DECLARATION