This document provides an introduction to regular expressions (regexes) using Perl and Python syntax. It covers common regex metacharacters like ^, $, ., \w, \d, and quantifiers like *, +, ?, {m,n}. Examples demonstrate how to use regexes to find and replace text, extract sections from files, and demultiplex sequencing data. The document recommends additional online resources for learning sed, grep, Perl, Python, and Vim regexes.