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- 1. Zhonghua Wang Miami, FL Tel: (305) 490-7294 E-Mail: Zhonghua_zho@hotmail.com ____________________________________________________________________________________ Education Florida International University, Miami, FL, Ph.D. in Chemistry and Biochemistry, 2004-2011 Peking University, China, B.S. in Life Sciences, 1999-2003 Experience Highlights 10+ years of hands-on lab research experience 4+ years of R&D experience with IVD reagents Small and large scale of cell culture and protein production experience Immunoassay reagent development to manufacturing transfer Leading multidisciplinary projects about proteins with strong leadership qualities Experienced in lab working and lab managing. Studies of interactions between biomolecules and small chemicals Studies of protein-microparticles conjugation QC analytical laboratory experience Molecular biology techniques such as molecular cloning, prokaryotes and eukaryotes cell culture, different scales of protein expression and purification, SDS-PAGE, IEF, ELISA, Western Blot, etc Analytical chemistry and biochemistry devices and instruments, including method development, equipment maintenance,etc Chromatography systems such as HPLC, LC-MS, AKTA,etc Spectrometry techniques such as CD,UV-Vis, fluorescence, etc Proficient in the use of statistic and plotting software, such asOffice, ChemDraw,OriginLab,Sigmaplot,etc Adaptive, initiative, independent, flexible and responsible, with good communication skills and teamwork spirit Professional Skills Molecular biology and cell biology Chemistry, biochemistry, and analytical chemistry Downstream protein purification process development and optimization Immunoassay development Liquid chromatography Hematology analyzer, flow cytometer and POC Data management and analysis, statistic tools and DOE NMR devices and instruments Experience Senior engineer (11/2011 ~ ) at Mindray North America, Miami center Small and large scale cell culture using FiberCell and Biovest AcuSyst-MAXimizer hollow fiber bioreactor systems. Small to Gram levels of Protein expression, purification and polishing using AKTA/Unicorn FPLC system, affinity, size exclusion and ion exchange chromatography, and TFF, normal flow and depth filtration.
- 2. Downstream purification process development and optimization including DOE and scale up/down. Experienced in production transferring from R&D bench to GMP manufacturing facility. Protein analysis using SDS-PAGE, Western blot, ELISA, Amicon and BCA assay. Immunoassay development using protein-nanoparticles conjugation. Reagent research and development for flow cytometer, hematology analyzer and POC. FDA Clinical evaluation of 3-part and 5- part differential Hematology analyzers. Lab management including supply ordering and instruments maintenance. Research assistant and projects designer (1/2005 ~09/2011) at Department of Chemistry and Biochemistry, Florida International University Lab manager for equipments maintenance and materials management. Primary designer and executor of three projects. Research area includes chemistry and biochemistry researches using different techniques such as molecular cloning, cell culture, protein expression and purification, 2D NMR, FPLC, HPLC, LC-MS, qPCR, SDS-PAGE, Western blot, ELISA, Amicon, BCA assay, HTRF, protein-nanoparticles conjugation, UV-Vis spectrometer,osmometer, etc. Built strategic plans, planned schedule, controlled the cost, gathered, filtered and analyzed relating information, ordered and purchased instruments and reagents, led the group to exert the assays and data analysis. Supervised four undergraduate researchers. Enhanced leadership, innovation and capability of overall planning, highly valued by mentor and other experts. Lab instructor and Teaching Assistant (8/2004~7/2011) at Department of Chemistry and Biochemistry, Florida International University Instructor of: "Chemistry and Society", "Survey of Chemistry", General Chemistry Lab, General Chemistry II,and Biochemistry lab. Experienced in teaching and training of chemistry and biochemistry related fields for different level students. Enhanced teamwork spirit and the ability of communication and expression, highly valued by students and supervisors. Winner of the Best Teaching Assistant award of Fall, 2006 (an award based on student and faculty evaluations). Research assistant (1/2004~8/2004) at NCPC Orchid Pharmaceuticals Co. Ltd. QC in pharmaceutical industry. Worked hard during internship, popularity in the company, highly valued by project leader. Enhanced teamwork skills and cooperation skills greatly. Set up and facilitated a cleanroom in the new pharmaceutical Lab. Research assistant (1/2003-1/2004) at Laboratory of Protein Engineering, Peking University Projects related to breast cancer, leukemia, lymphoma and Alzheimer's disease using 2D gel electrophoresis and flow cytometry. Quick learning from other group members and enhanced experimental skills.
- 3. Satisfying and promising accomplishment of desired tasks, highly valued by instructor and coworkers. Awards and Honors: Member of American Association for Clinical Chemistry International Clinical Cytometry Society member American Chemistry Society member FIU Chemistry Graduate Student Organizations vice president in 2006-2007 Winner of the Best Teaching Assistant award of Fall, 2006, Florida International University Winner of Academic Excellence Scholarship of Peking University in 2002 Publications 1. Wang, Zhonghua, et al., Isolation and structure determination of the modified heme in allylbenzene- inactivated chloroperoxidase. Abstracts of Papers, 238th ACS National Meeting, Washington, DC, United States,August 16-20, 2009 2. Tian, Hui; Wang, Zhonghua, et al., Relative stability of iron-sulfur bond vs. iron-nitrogen bond in heme thiolate protein: Evidence from an engineered cytochrome P450cam with a novel heme iron ligand set. Abstracts of Papers,238th ACS National Meeting, Washington, DC, United States,August 16-20, 2009 3. Tian, Hui; Wang, Zhonghua, et al., Single amino acid switch turns P450camfrom a monooxygenase to an efficient peroxidase. Abstracts of Papers, 237th ACS National Meeting, Salt Lake City, UT, United States,March 22-26, 2009 4. Wang, Zhonghua, et al., The Active Site Structure of Soybean Peroxidase as Probed by 1D and 2D Nuclear OverhauserEnhancement Effect.To be submitted. 5. Wang, Zhonghua, et al., Isolation and Structure Determination of a Novel Heme in Allylbenzene-Modified Chloroperoxidase. To be submitted. 6. Tian, Hui; Wang, Zhonghua, et al., Engineering Cytochrome P450 into P420 with Catalytic Activities. To be submitted. 7. Wang, Zhonghua, et al., 1 H-NMR Study of Paramagnetic Cytochrome P450cam-Cyanide Complexes: Assignment of Hyperfine-Shifted Heme Resonances. To be submitted. References Available upon request