![N.V.MINH 0975.515.383
B畛NH TCH 畉I TH畛 C畛A V畛T 畉 GY NHI畛M TH畛 NGHI畛M V畛I CH畛NG DEDSV-JXSP.
Ba con v畛t g但y b畛nh th畛c nghi畛m 達 動畛c m畛 kh叩m vo ngy th畛 5 sau khi b畛 nhi畛m b畛nh.
H狸nh A v B: Bu畛ng tr畛ng v l叩 l叩ch c畛a v畛t t畛 nh坦m kh担ng nhi畛m b畛nh (畛i ch畛ng).
H狸nh C,D v E: Quan s叩t th畉y tho叩i h坦a v xu畉t huy畉t nghi棚m tr畛ng 畛 bu畛ng tr畛ng 畛 t畉t c畉 c叩c con v畛t b畛
nhi畛m b畛nh. L叩ch h董i s動ng v sung huy畉t.
pigeon [16,20,21,25,27]. The virus-infected ducklings and
geese display various degrees of neurological signs, ranging
from recumbency to leg and wing paralysis. Affected birds
are culled or die from trampling in the field. It is noteworthy
that DEDSV has also been detected in chickens with egg
tural proteins, E and M (Figure 3A). Electron mic
virus-infected cells revealed viral particles in the
with a diameter of approximately 50 nm (Figure 3
As for all positive-strand RNA viruses, the D
nome is infectious [22,29]. To date, complete seq
Figure 2 Gross lesions of laying ducks experimentally infected with the DEDSV-JXSP strain. Three ducks were autopsied on day 5 after inf
B, The ovaries and spleens of ducks from the non-infected group (control). C E, Degeneration and severe hemorrhage in ovaries were obser
fected ducks.
GY B畛NH TH畛C NGHI畛M H畛I CH畛NG GI畉M 畉 - BYD](https://image.slidesharecdn.com/hoichunggiamdeovitupdate-200827040121/85/Hoi-chung-giam-de-o-vit-Minh-NV-24-320.jpg)
![TI LI畛U THAM KH畉O
N.V.MINH 0975.515.383
Duck Egg-Drop Syndrome Caused by BYD Virus, a New
Tembusu-Related Flavivirus
Jingliang Su1
*.
, Shuang Li1.
, Xudong Hu1,2.
, Xiuling Yu3
, Yongyue Wang1
, Peipei Liu2,4
, Xishan Lu1,2
,
Guozhong Zhang1
, Xueying Hu5
, Di Liu2,6
, Xiaoxia Li3
, Wenliang Su1
, Hao Lu2
, Ngai Shing Mok2
,
Peiyi Wang2
, Ming Wang1
, Kegong Tian3
*, George F. Gao2,4,7
*
1 Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China, 2CAS Key Laboratory of Pathogenic
Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 3 China Animal Disease Control Center, Beijing, China, 4Graduate
University, Chinese Academy of Sciences, Beijing, China, 5 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, 6Network Information Center,
Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 7 Beijing Institutes of Life Sciences, Chinese Academy of Sciences, Beijing, China
Abstract
Since April 2010, a severe outbreak of duck viral infection, with egg drop, feed uptake decline and ovary-oviduct disease,
has spread around the major duck-producing regions in China. A new virus, named BYD virus, was isolated in different areas,
and a similar disease was reproduced in healthy egg-producing ducks, infecting with the isolated virus. The virus was re-
isolated from the affected ducks and replicated well in primary duck embryo fibroblasts and Vero cells, causing the
cytopathic effect. The virus was identified as an enveloped positive-stranded RNA virus with a size of approximately 55 nm
in diameter. Genomic sequencing of the isolated virus revealed that it is closely related to Tembusu virus (a mosquito-borne
Ntaya group flavivirus), with 8791% nucleotide identity of the partial E (envelope) proteins to that of Tembusu virus and
72% of the entire genome coding sequence with Bagaza virus, the most closely related flavivirus with an entirely sequenced
genome. Collectively our systematic studies fulfill Kochs postulates, and therefore, the causative agent of the duck egg
drop syndrome occurring in China is a new flavivirus. Flavivirus is an emerging and re-emerging zoonotic pathogen and
BYD virus that causes severe egg-drop, could be disastrous for the duck industry. More importantly its public health
concerns should also be evaluated, and its epidemiology should be closely watched due to the zoonotic nature of
flaviviruses.
Citation: Su J, Li S, Hu X, Yu X, Wang Y, et al. (2011) Duck Egg-Drop Syndrome Caused by BYD Virus, a New Tembusu-Related Flavivirus. PLoS ONE 6(3): e18106.
doi:10.1371/journal.pone.0018106
Editor: Jianming Qiu, University of Kansas Medical Center, United States of America
Received December 22, 2010; Accepted February 21, 2011; Published March 24, 2011
Copyright: 2011 Su et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The work is supported by the China Ministry of Science and Technology (MOST) Project 973 (Grant No. 2010CB530301 and 2010CB530303). DL is partly
supported by the Natural Science Foundation of China (NSFC, Grant No. 30800640). GFG is a leading principal investigator of the NSFC Innovative Research Group
(Grant No. 81021003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing Interests: The authors have declared that no competing interests exist.
* E-mail: gaof@im.ac.cn (GFG); jinglsu@yahoo.com (JS); tiankg@263.net (KT)
. These authors contribute equally to this work.
Introduction
Duck farming is a traditional agro-business in China and
approximately 10.5 kb in size, encodes three structural proteins
[capsid (C), membrane (PrM and M) and envelope (E)] and seven
non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B
REVIEW doi: 10.1007/s11427-013-4515-z
Progress of Projects Supported by NSFC
Duck egg drop syndrome virus: an emerging Tembusu-related
flavivirus in China
LIU PeiPei1,4
, LU Hao3
, LI Shuang2
, WU Ying1
, George Fu GAO1,4,5,6*
& SU JingLiang2*
1
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101,
China;
2
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural Univer-
sity, Beijing 100193, China;
3
Bureau of Life Sciences and Biotechnology, Chinese Academy of Sciences, Beijing 100864, China;
4
University of Chinese Academy of Sciences, Beijing 100049, China;
5
National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
6
Research Network of Immunity and Health, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China
Received May 28, 2013; accepted June 15, 2013
Duck egg drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus isolated from ducks in China. DEDSV in-
fection mainly results in severe egg drop syndrome in domestic poultry, which leads to huge economic losses. Thus, the dis-
covery of ways and means to combat DEDSV is urgent. Since 2010, a remarkable amount of progress concerning DEDSV re-
search has been achieved. Here, we review current knowledge on the epidemiology, symptomatology, and pathology of
DEDSV. A detailed dissection of the viral genome and polyprotein sequences, comparative analysis of viral antigenicity and
the corresponding potential immunity against the virus are also summarized. Current findings indicate that DEDSV should be a
distinct species from Tembusu virus. Moreover, the adaption of DEDSV in wildlife and its high homology to pathogenic fla-
viviruses (e.g., West Nile virus, Japanese encephalitis virus, and dengue virus), illustrate its reemergence and potential to be-
come a zoonotic pathogen that should not be overlooked. Detailed insight into the antigenicity and corresponding immunity
against the virus is of clear significance for the development of vaccines and antiviral drugs specific for DEDSV.
DEDSV, BYDV, pathology, genome, phylogeny, antigenicity, vaccine
Citation: Liu P P, Lu H, Li S, et al. Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China. Sci China Life Sci, 2013, 56: 701710,
doi: 10.1007/s11427-013-4515-z](https://image.slidesharecdn.com/hoichunggiamdeovitupdate-200827040121/85/Hoi-chung-giam-de-o-vit-Minh-NV-29-320.jpg)
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- 22. GY B畛NH TH畛C NGHI畛M H畛I CH畛NG GI畉M 畉 - BYD N.V.MINH 0975.515.383 Figure 1. Regions of BYDV infection outbreaks in China. The provinces or autonomous cities (regions) affected are indicated in gray. Regions from which viruses were isolated and comfirmed by RT-PCR/sequencing are labeled with triangles. doi:10.1371/journal.pone.0018106.g001 Figure 2. The course of natural disease. Representative of the average feed uptake of the infected flock at Farm 1 (A); Daily egg production rate represented by two infected duck farms (B). The flocks at Farm 1 were 35 weeks old, and Farm 2 flocks 14 (Farm 2-F1, -F2, F3, -F4) are four different flocks (76 weeks old) at another representative farm. doi:10.1371/journal.pone.0018106.g002 PLoS ONE | www.plosone.org 3 March 2011 | Volume 6 | Issue 3 | e18106 端 H狸nh A: Bi畛u 畛 s畛 h畉p thu th畛c n trung b狸nh c畛a n b畛 nhi畛m b畛nh t畉i trang tr畉i 端 H狸nh B: T畛 l畛 s畉n xu畉t tr畛ng hng ngy 動畛c 畉i di畛n b畛i hai trang tr畉i v畛t b畛 nhi畛m b畛nh (B). n 畛 Farm 1 l炭c 35 tu畉n tu畛i n 畛 Farm 2 l b畛n n kh叩c nhau (F1-4 畛 76 tu畉n tu畛i) t畉i m畛t trang tr畉i kh叩c 畉NH H働畛NG C畛A H畛I CH畛NG GI畉M 畉 TRN V畛T DO M畉C T畛 NHIN.
- 23. N.V.MINH 0975.515.383 端 T畛 l畛 畉 hng ngy tr動畛c v sau khi nhi畛m b畛nh th畛c nghi畛m. 端 S畛 suy gi畉m t畛 l畛 畉 sau khi g但y b畛nh th畛c nghi畛m l hi畛n nhi棚n, t動董ng t畛 nh動 nhi畛m b畛nh t畛 nhi棚n. 畉NH H働畛NG C畛A H畛I CH畛NG GI畉M 畉 TRN V畛T KHI GY B畛NH TH畛C NGHI畛M Figure 5. Daily egg production rate before and after experimental infection. The decline of egg p natural infection. A New Flav GY B畛NH TH畛C NGHI畛M H畛I CH畛NG GI畉M 畉 - BYD
- 24. N.V.MINH 0975.515.383 B畛NH TCH 畉I TH畛 C畛A V畛T 畉 GY NHI畛M TH畛 NGHI畛M V畛I CH畛NG DEDSV-JXSP. Ba con v畛t g但y b畛nh th畛c nghi畛m 達 動畛c m畛 kh叩m vo ngy th畛 5 sau khi b畛 nhi畛m b畛nh. H狸nh A v B: Bu畛ng tr畛ng v l叩 l叩ch c畛a v畛t t畛 nh坦m kh担ng nhi畛m b畛nh (畛i ch畛ng). H狸nh C,D v E: Quan s叩t th畉y tho叩i h坦a v xu畉t huy畉t nghi棚m tr畛ng 畛 bu畛ng tr畛ng 畛 t畉t c畉 c叩c con v畛t b畛 nhi畛m b畛nh. L叩ch h董i s動ng v sung huy畉t. pigeon [16,20,21,25,27]. The virus-infected ducklings and geese display various degrees of neurological signs, ranging from recumbency to leg and wing paralysis. Affected birds are culled or die from trampling in the field. It is noteworthy that DEDSV has also been detected in chickens with egg tural proteins, E and M (Figure 3A). Electron mic virus-infected cells revealed viral particles in the with a diameter of approximately 50 nm (Figure 3 As for all positive-strand RNA viruses, the D nome is infectious [22,29]. To date, complete seq Figure 2 Gross lesions of laying ducks experimentally infected with the DEDSV-JXSP strain. Three ducks were autopsied on day 5 after inf B, The ovaries and spleens of ducks from the non-infected group (control). C E, Degeneration and severe hemorrhage in ovaries were obser fected ducks. GY B畛NH TH畛C NGHI畛M H畛I CH畛NG GI畉M 畉 - BYD
- 25. N.V.MINH 0975.515.383Figure 3. Gross lesions and histopathology of the clinical samples. Mild hemorrhage of ovarian follicles (a (A); Severe hamorrhage and regression of ovarian follicles (B); HE stained ovary section, the follicles ruptured A New Flavivirus B畛nh t鱈ch 畉i th畛 v vi th畛 c畛a c叩c m畉u b畛nh ph畉m l但m sng. 端 H狸nh A: Nang no達n xu畉t huy畉t nh畉 c畛a (m滴i t棚n) trong giai o畉n 畉u c畛a nhi畛m tr湛ng; 端 H狸nh B: Xu畉t huy畉t nghi棚m tr畛ng 畛 bu畛ng tr炭ng v c叩c nang no達n 端 H狸nh C: Nhu畛m HE ph畉n bu畛ng tr畛ng, c叩c nang v畛 v c叩c m担 ch畛a 畉y c叩c b畉ch c畉u 叩i toan tr嘆n ho畉c h畉t (C). 端 H狸nh D: Nhu畛m mu HE: Tng sinh t畉 bo th畉n kinh 畛m 畛 n達o, th但m nhi畛m t畉 bo lympho 畛 mng nh畛n GY B畛NH TH畛C NGHI畛M H畛I CH畛NG GI畉M 畉 - BYD
- 26. N.V.MINH 0975.515.383 the production records were checked for several affected flocks in different areas. To exclude avian influenza virus infection, serum samples were collected from the affected flocks, and antibody titers against H5 subtype avian influenza A virus were tested with hemagglutination inhibition (HI) assays according to the standard method (http://www.oie.int/eng/normes/mmanual/2008/pdf/2. 03.04_AI.pdf). Ducks in the affected flocks showing morbidity or death within 6 hrs were taken to our laboratory for necropsy. Tissue samples were fixed in 10% neutral buffered formalin solution for histopathology. Simultaneously, brain, ovary and spleen tissues Figure 6. Pathological changes of the experimentally-infected ducks. Mock-infected control (A); Severe hemorrhage and regression of ovarian follicles (B); Spleens from mock-infected control (left) and enlarged spleens from the infected ducks (C); HE-stained ovary section showing 端 H狸nh A: 畛i ch畛ng 端 H狸nh B: Xu畉t huy畉t n畉ng v tho叩i ho叩 nang no達n (B); 端 H狸nh C: L叩 l叩ch t畛 v畛t 畛i ch畛ng (tr叩i) v l叩 l叩ch s動ng to t畛 v畛t b畛 nhi畛m b畛nh 端 H狸nh D: Ph畉n bu畛ng tr畛ng nhu畛m mu HE cho th畉y xu畉t huy畉t v v畛 nang tr畛ng nhu畛m mu 畛 端 H狸nh E: Ph畉n n達o nhu畛m HE ti畉t l畛 t畉 bo lympho v th但m nhi畛m t畉 bo 董n nh但n d動畛i mng c畛ng s畛 n達o. 端 H狸nh F: Tng sinh t畉 bo th畉n kinh 畛m 畛 n達o(m滴i t棚n) NH畛NG THAY 畛I B畛NH L C畛A V畛T B畛 GY B畛NH TH畛C NGHI畛M. GY B畛NH TH畛C NGHI畛M H畛I CH畛NG GI畉M 畉 - BYD
- 27. H畛I CH畛NG GI畉M 畉 TRN V畛T/Bi畛n ph叩p ph嘆ng b畛nh N.V.MINH 0975.515.383 B畛nh do Tembusu virus (BYD) ch動a c坦 v畉c xin ph嘆ng b畛nh l動u hnh hnh ch鱈nh th畛c 畛 Vi畛t Nam), v狸 v畉y ng動畛i chn nu担i c畉n ch畛 畛ng th畛c hi畛n t畛t c叩c bi畛n ph叩p ATSH nh動 v畛 sinh, 畛nh k畛 s叩t tr湛ng chu畛ng tr畉i v khu v畛c chn nu担i, chm s坦c nu担i d動畛ng t畛t. 1. Tu但n th畛 v th畛c hi畛n nghi棚m ng畉t ch動董ng tr狸nh vaccine ph嘆ng b畛nh tr棚n v畛t 畉 2. Tng c動畛ng tr畛 s畛c, tr畛 l畛c cho n v畛t b畉ng c叩c lo畉i thu畛c b畛 tr畛 (Vitamin, kho叩ng ch畉t) v men ti棚u ho叩 s畛ng 3. Khi th畛i ti畉t thay 畛i c畉n ch畛 畛ng i畛u tr畛 d畛 ph嘆ng c叩c b畛nh nhi畛m khu畉n b畉ng kh叩ng sinh, Gluco, Vitamin C. 4. Ki畛m so叩t v th畛c hi畛n t畛t c叩c bi畛n ph叩p an ton sinh h畛c trong chn nu担i - Phun s叩t tr湛ng chu畛ng tr畉i 畛nh k畛 1 2 l畉n/tu畉n - Ph叩t quang b畛i d畉m, c但y c畛 v kh董i th担ng c担ng r達nh - Di畛t b畛 g畉y v 畛nh k畛 di畛t ru畛i mu畛i trong v ngoi chu畛ng nu担i 5. N畉u c坦 vaccine ti棚m ph嘆ng: V畛t con ti棚m t畛 7 21 ngy, v畛t 畉 6 th叩ng ti棚m nh畉c l畉i 1 l畉n
- 28. H畛I CH畛NG GI畉M 畉 TRN V畛T/Bi畛n ph叩p ph嘆ng b畛nh N.V.MINH 0975.515.383 M畛T S畛 L働U KHI S畛 D畛NG VACCINE T畛 TRUNG QU畛C (Ch動a 動畛c ng k箪 l動u hnh) 端 C叩c VACCINE S畛NG nh畉p ti畛u ng畉ch s畉 lm tng nguy c董 l動u hnh v ph叩t t叩n d畛ch b畛nh 畛 Vi畛t Nam C畉n c但n nh畉c th畉n tr畛ng khi s畛 d畛ng. 端 H畉u h畉t vaccine BYD s畛ng c畉n i畛u ki畛n b畉o qu畉n t畛i 動u (-15 畛 C), khi mua hng tr担i n畛i s畉 kh担ng 畉m b畉o ch畉t l動畛ng ti棚m ph嘆ng. 端 N畉u b畉t bu畛c ph畉i s畛 d畛ng vaccine 畛 ph嘆ng b畛nh, n棚n l畛a ch畛n vaccine BYD ch畉t 畛 畉m b畉o an ton cho n gi畛ng v kh担ng lm gia tang nguy c董 b湛ng ph叩t d畛ch VX CH畉T VACCINE S畛NG
- 29. TI LI畛U THAM KH畉O N.V.MINH 0975.515.383 Duck Egg-Drop Syndrome Caused by BYD Virus, a New Tembusu-Related Flavivirus Jingliang Su1 *. , Shuang Li1. , Xudong Hu1,2. , Xiuling Yu3 , Yongyue Wang1 , Peipei Liu2,4 , Xishan Lu1,2 , Guozhong Zhang1 , Xueying Hu5 , Di Liu2,6 , Xiaoxia Li3 , Wenliang Su1 , Hao Lu2 , Ngai Shing Mok2 , Peiyi Wang2 , Ming Wang1 , Kegong Tian3 *, George F. Gao2,4,7 * 1 Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China, 2CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 3 China Animal Disease Control Center, Beijing, China, 4Graduate University, Chinese Academy of Sciences, Beijing, China, 5 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China, 6Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, 7 Beijing Institutes of Life Sciences, Chinese Academy of Sciences, Beijing, China Abstract Since April 2010, a severe outbreak of duck viral infection, with egg drop, feed uptake decline and ovary-oviduct disease, has spread around the major duck-producing regions in China. A new virus, named BYD virus, was isolated in different areas, and a similar disease was reproduced in healthy egg-producing ducks, infecting with the isolated virus. The virus was re- isolated from the affected ducks and replicated well in primary duck embryo fibroblasts and Vero cells, causing the cytopathic effect. The virus was identified as an enveloped positive-stranded RNA virus with a size of approximately 55 nm in diameter. Genomic sequencing of the isolated virus revealed that it is closely related to Tembusu virus (a mosquito-borne Ntaya group flavivirus), with 8791% nucleotide identity of the partial E (envelope) proteins to that of Tembusu virus and 72% of the entire genome coding sequence with Bagaza virus, the most closely related flavivirus with an entirely sequenced genome. Collectively our systematic studies fulfill Kochs postulates, and therefore, the causative agent of the duck egg drop syndrome occurring in China is a new flavivirus. Flavivirus is an emerging and re-emerging zoonotic pathogen and BYD virus that causes severe egg-drop, could be disastrous for the duck industry. More importantly its public health concerns should also be evaluated, and its epidemiology should be closely watched due to the zoonotic nature of flaviviruses. Citation: Su J, Li S, Hu X, Yu X, Wang Y, et al. (2011) Duck Egg-Drop Syndrome Caused by BYD Virus, a New Tembusu-Related Flavivirus. PLoS ONE 6(3): e18106. doi:10.1371/journal.pone.0018106 Editor: Jianming Qiu, University of Kansas Medical Center, United States of America Received December 22, 2010; Accepted February 21, 2011; Published March 24, 2011 Copyright: 2011 Su et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The work is supported by the China Ministry of Science and Technology (MOST) Project 973 (Grant No. 2010CB530301 and 2010CB530303). DL is partly supported by the Natural Science Foundation of China (NSFC, Grant No. 30800640). GFG is a leading principal investigator of the NSFC Innovative Research Group (Grant No. 81021003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: gaof@im.ac.cn (GFG); jinglsu@yahoo.com (JS); tiankg@263.net (KT) . These authors contribute equally to this work. Introduction Duck farming is a traditional agro-business in China and approximately 10.5 kb in size, encodes three structural proteins [capsid (C), membrane (PrM and M) and envelope (E)] and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B REVIEW doi: 10.1007/s11427-013-4515-z Progress of Projects Supported by NSFC Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China LIU PeiPei1,4 , LU Hao3 , LI Shuang2 , WU Ying1 , George Fu GAO1,4,5,6* & SU JingLiang2* 1 CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; 2 Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural Univer- sity, Beijing 100193, China; 3 Bureau of Life Sciences and Biotechnology, Chinese Academy of Sciences, Beijing 100864, China; 4 University of Chinese Academy of Sciences, Beijing 100049, China; 5 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 6 Research Network of Immunity and Health, Beijing Institutes of Life Science, Chinese Academy of Sciences, Beijing 100101, China Received May 28, 2013; accepted June 15, 2013 Duck egg drop syndrome virus (DEDSV) is a newly emerging pathogenic flavivirus isolated from ducks in China. DEDSV in- fection mainly results in severe egg drop syndrome in domestic poultry, which leads to huge economic losses. Thus, the dis- covery of ways and means to combat DEDSV is urgent. Since 2010, a remarkable amount of progress concerning DEDSV re- search has been achieved. Here, we review current knowledge on the epidemiology, symptomatology, and pathology of DEDSV. A detailed dissection of the viral genome and polyprotein sequences, comparative analysis of viral antigenicity and the corresponding potential immunity against the virus are also summarized. Current findings indicate that DEDSV should be a distinct species from Tembusu virus. Moreover, the adaption of DEDSV in wildlife and its high homology to pathogenic fla- viviruses (e.g., West Nile virus, Japanese encephalitis virus, and dengue virus), illustrate its reemergence and potential to be- come a zoonotic pathogen that should not be overlooked. Detailed insight into the antigenicity and corresponding immunity against the virus is of clear significance for the development of vaccines and antiviral drugs specific for DEDSV. DEDSV, BYDV, pathology, genome, phylogeny, antigenicity, vaccine Citation: Liu P P, Lu H, Li S, et al. Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China. Sci China Life Sci, 2013, 56: 701710, doi: 10.1007/s11427-013-4515-z